Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents
Diana Lomelí-Marroquín,1 David Medina Cruz,2,3 Alfonso Nieto-Argüello,1 Ada Vernet Crua,2,3 Junjiang Chen,2,3 Alejandro Torres-Castro,4 Thomas J Webster,2,3 Jorge L Cholula-Díaz1 1School of Engineering and Sciences, Tecnologico de Monterrey, Monterrey, NL 6...
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Dove Medical Press
2019
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oai:doaj.org-article:72ce6dcc99e14dcc815b6d132e80bb7c2021-12-02T06:48:15ZStarch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents1178-2013https://doaj.org/article/72ce6dcc99e14dcc815b6d132e80bb7c2019-03-01T00:00:00Zhttps://www.dovepress.com/starch-mediated-synthesis-of-mono--and-bimetallic-silvergold-nanoparti-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Diana Lomelí-Marroquín,1 David Medina Cruz,2,3 Alfonso Nieto-Argüello,1 Ada Vernet Crua,2,3 Junjiang Chen,2,3 Alejandro Torres-Castro,4 Thomas J Webster,2,3 Jorge L Cholula-Díaz1 1School of Engineering and Sciences, Tecnologico de Monterrey, Monterrey, NL 64849, Mexico; 2Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA; 3Nanomedicine Science and Technology Center, Northeastern University, Boston, MA 02115, USA; 4Faculty School of Mechanical and Electrical Engineering (FIME), Autonomous University of Nuevo Leon (UANL), San Nicolás de los Garza, NL 66451, Mexico Background and aim: Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells. Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent. Methods: The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)–visible spectroscopy, dynamic light scattering (DLS) and ζ-potential. The presence of starch as capping agent was determined by Fourier transform infrared (FT-IR) spectroscopy. The structural properties were studied by X-ray diffraction (XRD). Transmission electron microscopy (TEM) imaging was done to determine the morphology and size of the nanostructures. The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis. To further study the biomedical applications of the synthesized nanostructures, antibacterial studies against multidrug-resistant (MDR) Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) were conducted. In addition, the NPs were added to the growth media of human dermal fibroblast (HDF) and human melanoma cells to show their cytocompatibility and cytotoxicity, respectively, over a 3-day experiment. Results: UV–visible spectroscopy confirmed the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs. The NPs showed a face-centered cubic crystal structure and an icosahedral shape with mean particle sizes of 28.5 and 9.7 nm for AuNPs and Ag/AuNPs, respectively. The antibacterial studies of the NPs against antibiotic-resistant bacterial strains presented a dose-dependent antimicrobial behavior. Furthermore, the NPs showed cytocompatibility towards HDF, but a dose-dependent anticancer effect was found when human melanoma cells were grown in presence of different NP concentrations for 72 hours. Conclusion: In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications. Keywords: green nanotechnology, noble metal alloy nanoparticles, nanomedicine, physicochemical propertiesLomelí-Marroquín DMedina Cruz DNieto-Argüello AVernet Crua AChen JTorres-Castro AWebster TJCholula-Díaz JLDove Medical Pressarticlegreen nanotechnologynoble metal alloy nanoparticlesnanomedicinephysicochemical propertiesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 2171-2190 (2019) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
green nanotechnology noble metal alloy nanoparticles nanomedicine physicochemical properties Medicine (General) R5-920 |
| spellingShingle |
green nanotechnology noble metal alloy nanoparticles nanomedicine physicochemical properties Medicine (General) R5-920 Lomelí-Marroquín D Medina Cruz D Nieto-Argüello A Vernet Crua A Chen J Torres-Castro A Webster TJ Cholula-Díaz JL Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents |
| description |
Diana Lomelí-Marroquín,1 David Medina Cruz,2,3 Alfonso Nieto-Argüello,1 Ada Vernet Crua,2,3 Junjiang Chen,2,3 Alejandro Torres-Castro,4 Thomas J Webster,2,3 Jorge L Cholula-Díaz1 1School of Engineering and Sciences, Tecnologico de Monterrey, Monterrey, NL 64849, Mexico; 2Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA; 3Nanomedicine Science and Technology Center, Northeastern University, Boston, MA 02115, USA; 4Faculty School of Mechanical and Electrical Engineering (FIME), Autonomous University of Nuevo Leon (UANL), San Nicolás de los Garza, NL 66451, Mexico Background and aim: Bimetallic silver/gold nanosystems are expected to significantly improve therapeutic efficacy compared to their monometallic counterparts by maintaining the general biocompatibility of gold nanoparticles (AuNPs) while, at the same time, decreasing the relatively high toxicity of silver nanoparticles (AgNPs) toward healthy human cells. Thus, the aim of this research was to establish a highly reproducible one-pot green synthesis of colloidal AuNPs and bimetallic Ag/Au alloy nanoparticles (NPs; Ag/AuNPs) using starch as reducing and capping agent. Methods: The optical properties, high reproducibility, stability and particle size distribution of the colloidal NPs were analyzed by ultraviolet (UV)–visible spectroscopy, dynamic light scattering (DLS) and ζ-potential. The presence of starch as capping agent was determined by Fourier transform infrared (FT-IR) spectroscopy. The structural properties were studied by X-ray diffraction (XRD). Transmission electron microscopy (TEM) imaging was done to determine the morphology and size of the nanostructures. The chemical composition of the nanomaterials was determined by energy-dispersive X-ray spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS) analysis. To further study the biomedical applications of the synthesized nanostructures, antibacterial studies against multidrug-resistant (MDR) Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) were conducted. In addition, the NPs were added to the growth media of human dermal fibroblast (HDF) and human melanoma cells to show their cytocompatibility and cytotoxicity, respectively, over a 3-day experiment. Results: UV–visible spectroscopy confirmed the highly reproducible green synthesis of colloidal AuNPs and Ag/AuNPs. The NPs showed a face-centered cubic crystal structure and an icosahedral shape with mean particle sizes of 28.5 and 9.7 nm for AuNPs and Ag/AuNPs, respectively. The antibacterial studies of the NPs against antibiotic-resistant bacterial strains presented a dose-dependent antimicrobial behavior. Furthermore, the NPs showed cytocompatibility towards HDF, but a dose-dependent anticancer effect was found when human melanoma cells were grown in presence of different NP concentrations for 72 hours. Conclusion: In this study, mono- and bimetallic NPs were synthesized for the first time using a highly reproducible, environmentally friendly, cost-effective and quick method and were successfully characterized and tested for several anti-infection and anticancer biomedical applications. Keywords: green nanotechnology, noble metal alloy nanoparticles, nanomedicine, physicochemical properties |
| format |
article |
| author |
Lomelí-Marroquín D Medina Cruz D Nieto-Argüello A Vernet Crua A Chen J Torres-Castro A Webster TJ Cholula-Díaz JL |
| author_facet |
Lomelí-Marroquín D Medina Cruz D Nieto-Argüello A Vernet Crua A Chen J Torres-Castro A Webster TJ Cholula-Díaz JL |
| author_sort |
Lomelí-Marroquín D |
| title |
Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents |
| title_short |
Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents |
| title_full |
Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents |
| title_fullStr |
Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents |
| title_full_unstemmed |
Starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents |
| title_sort |
starch-mediated synthesis of mono- and bimetallic silver/gold nanoparticles as antimicrobial and anticancer agents |
| publisher |
Dove Medical Press |
| publishDate |
2019 |
| url |
https://doaj.org/article/72ce6dcc99e14dcc815b6d132e80bb7c |
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