Potential for cross-contamination of diatom DNA samples when using toothbrushes

The use of toothbrushes and similar devices for sampling diatoms from hard surfaces is a well-established approach. Toothbrushes are routinely cleaned and reused when sampling for analysis by light microscopy. This paper looks at the scale of contamination encountered when this techn...

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Autores principales:	Martyn G. Kelly, Tim Jones, Kerry Walsh
Formato:	article
Lenguaje:	EN
Publicado:	Pensoft Publishers 2021
Materias:	Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/72cffc44e252414a9e92416b9982c8f5
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spelling	oai:doaj.org-article:72cffc44e252414a9e92416b9982c8f52021-12-02T19:17:14ZPotential for cross-contamination of diatom DNA samples when using toothbrushes10.3897/mbmg.5.665032534-9708https://doaj.org/article/72cffc44e252414a9e92416b9982c8f52021-05-01T00:00:00Zhttps://mbmg.pensoft.net/article/66503/download/pdf/https://mbmg.pensoft.net/article/66503/download/xml/https://mbmg.pensoft.net/article/66503/https://doaj.org/toc/2534-9708 The use of toothbrushes and similar devices for sampling diatoms from hard surfaces is a well-established approach. Toothbrushes are routinely cleaned and reused when sampling for analysis by light microscopy. This paper looks at the scale of contamination encountered when this technique is used to sample diatoms for metabarcoding analyses, as well as at the scale of contamination to be expected if stream, rather than distilled water, is used to wash diatoms from stones. Although some contamination attributable to toothbrushes was detected, read numbers were low and had no effect on index calculation or ecological status estimates. However, if the primary focus of a study is to thoroughly document diversity in a sample, then even this small level of contamination may be unacceptable and more stringent measures may be required. Martyn G. KellyTim JonesKerry WalshPensoft PublishersarticleEcologyQH540-549.5ENMetabarcoding and Metagenomics, Vol 5, Iss , Pp 51-57 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ecology QH540-549.5
spellingShingle	Ecology QH540-549.5 Martyn G. Kelly Tim Jones Kerry Walsh Potential for cross-contamination of diatom DNA samples when using toothbrushes
description	The use of toothbrushes and similar devices for sampling diatoms from hard surfaces is a well-established approach. Toothbrushes are routinely cleaned and reused when sampling for analysis by light microscopy. This paper looks at the scale of contamination encountered when this technique is used to sample diatoms for metabarcoding analyses, as well as at the scale of contamination to be expected if stream, rather than distilled water, is used to wash diatoms from stones. Although some contamination attributable to toothbrushes was detected, read numbers were low and had no effect on index calculation or ecological status estimates. However, if the primary focus of a study is to thoroughly document diversity in a sample, then even this small level of contamination may be unacceptable and more stringent measures may be required.
format	article
author	Martyn G. Kelly Tim Jones Kerry Walsh
author_facet	Martyn G. Kelly Tim Jones Kerry Walsh
author_sort	Martyn G. Kelly
title	Potential for cross-contamination of diatom DNA samples when using toothbrushes
title_short	Potential for cross-contamination of diatom DNA samples when using toothbrushes
title_full	Potential for cross-contamination of diatom DNA samples when using toothbrushes
title_fullStr	Potential for cross-contamination of diatom DNA samples when using toothbrushes
title_full_unstemmed	Potential for cross-contamination of diatom DNA samples when using toothbrushes
title_sort	potential for cross-contamination of diatom dna samples when using toothbrushes
publisher	Pensoft Publishers
publishDate	2021
url	https://doaj.org/article/72cffc44e252414a9e92416b9982c8f5
work_keys_str_mv	AT martyngkelly potentialforcrosscontaminationofdiatomdnasampleswhenusingtoothbrushes AT timjones potentialforcrosscontaminationofdiatomdnasampleswhenusingtoothbrushes AT kerrywalsh potentialforcrosscontaminationofdiatomdnasampleswhenusingtoothbrushes
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Potential for cross-contamination of diatom DNA samples when using toothbrushes

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