Identification of conditionally essential genes for Streptococcus suis infection in pigs

Streptococcus suis is a Gram-positive bacterium and zoonotic pathogen that causes meningitis and sepsis in pigs and humans. The aim of this study was to identify genes required for S. suis infection. We created Tn-Seq libraries in a virulent S. suis strain 10, which was used to inoculate pigs in an...

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Autores principales:	Jesús Arenas, Aldert Zomer, Jose Harders-Westerveen, Hester J. Bootsma, Marien I. De Jonge, Norbert Stockhofe-Zurwieden, Hilde E. Smith, Astrid De Greeff
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	streptococcus suis pathogenesis zoonotic pathogen infection transposon mutagenesis tn-seq Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/72d2fb825c684c63b25cceea4863ed21
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spelling	oai:doaj.org-article:72d2fb825c684c63b25cceea4863ed212021-11-17T14:21:58ZIdentification of conditionally essential genes for Streptococcus suis infection in pigs2150-55942150-560810.1080/21505594.2020.1764173https://doaj.org/article/72d2fb825c684c63b25cceea4863ed212020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1764173https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Streptococcus suis is a Gram-positive bacterium and zoonotic pathogen that causes meningitis and sepsis in pigs and humans. The aim of this study was to identify genes required for S. suis infection. We created Tn-Seq libraries in a virulent S. suis strain 10, which was used to inoculate pigs in an intrathecal experimental infection. Comparative analysis of the relative abundance of mutants recovered from different sites of infection (blood, cerebrospinal fluid, and meninges of the brain) identified 361 conditionally essential genes, i.e. required for infection, which is about 18% of the genome. The conditionally essential genes were primarily involved in metabolic and transport processes, regulation, ribosomal structure and biogenesis, transcription, and cell wall membrane and envelope biogenesis, stress defenses, and immune evasion. Directed mutants were created in a set of 10 genes of different genetic ontologies and their role was determined in ex vivo models. Mutants showed different levels of sensitivity to survival in whole blood, serum, cerebrospinal fluid, thermic shock, and stress conditions, as compared to the wild type. Additionally, the role of three selected mutants was validated in co-infection experiments in which pigs were infected with both wild type and isogenic mutant strains. The genetic determinants of infection identified in this work contribute to novel insights in S. suis pathogenesis and could serve as targets for novel vaccines or antimicrobial drugs.Jesús ArenasAldert ZomerJose Harders-WesterveenHester J. BootsmaMarien I. De JongeNorbert Stockhofe-ZurwiedenHilde E. SmithAstrid De GreeffTaylor & Francis Grouparticlestreptococcus suispathogenesiszoonotic pathogeninfectiontransposon mutagenesistn-seqInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 446-464 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	streptococcus suis pathogenesis zoonotic pathogen infection transposon mutagenesis tn-seq Infectious and parasitic diseases RC109-216
spellingShingle	streptococcus suis pathogenesis zoonotic pathogen infection transposon mutagenesis tn-seq Infectious and parasitic diseases RC109-216 Jesús Arenas Aldert Zomer Jose Harders-Westerveen Hester J. Bootsma Marien I. De Jonge Norbert Stockhofe-Zurwieden Hilde E. Smith Astrid De Greeff Identification of conditionally essential genes for Streptococcus suis infection in pigs
description	Streptococcus suis is a Gram-positive bacterium and zoonotic pathogen that causes meningitis and sepsis in pigs and humans. The aim of this study was to identify genes required for S. suis infection. We created Tn-Seq libraries in a virulent S. suis strain 10, which was used to inoculate pigs in an intrathecal experimental infection. Comparative analysis of the relative abundance of mutants recovered from different sites of infection (blood, cerebrospinal fluid, and meninges of the brain) identified 361 conditionally essential genes, i.e. required for infection, which is about 18% of the genome. The conditionally essential genes were primarily involved in metabolic and transport processes, regulation, ribosomal structure and biogenesis, transcription, and cell wall membrane and envelope biogenesis, stress defenses, and immune evasion. Directed mutants were created in a set of 10 genes of different genetic ontologies and their role was determined in ex vivo models. Mutants showed different levels of sensitivity to survival in whole blood, serum, cerebrospinal fluid, thermic shock, and stress conditions, as compared to the wild type. Additionally, the role of three selected mutants was validated in co-infection experiments in which pigs were infected with both wild type and isogenic mutant strains. The genetic determinants of infection identified in this work contribute to novel insights in S. suis pathogenesis and could serve as targets for novel vaccines or antimicrobial drugs.
format	article
author	Jesús Arenas Aldert Zomer Jose Harders-Westerveen Hester J. Bootsma Marien I. De Jonge Norbert Stockhofe-Zurwieden Hilde E. Smith Astrid De Greeff
author_facet	Jesús Arenas Aldert Zomer Jose Harders-Westerveen Hester J. Bootsma Marien I. De Jonge Norbert Stockhofe-Zurwieden Hilde E. Smith Astrid De Greeff
author_sort	Jesús Arenas
title	Identification of conditionally essential genes for Streptococcus suis infection in pigs
title_short	Identification of conditionally essential genes for Streptococcus suis infection in pigs
title_full	Identification of conditionally essential genes for Streptococcus suis infection in pigs
title_fullStr	Identification of conditionally essential genes for Streptococcus suis infection in pigs
title_full_unstemmed	Identification of conditionally essential genes for Streptococcus suis infection in pigs
title_sort	identification of conditionally essential genes for streptococcus suis infection in pigs
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/72d2fb825c684c63b25cceea4863ed21
work_keys_str_mv	AT jesusarenas identificationofconditionallyessentialgenesforstreptococcussuisinfectioninpigs AT aldertzomer identificationofconditionallyessentialgenesforstreptococcussuisinfectioninpigs AT joseharderswesterveen identificationofconditionallyessentialgenesforstreptococcussuisinfectioninpigs AT hesterjbootsma identificationofconditionallyessentialgenesforstreptococcussuisinfectioninpigs AT marienidejonge identificationofconditionallyessentialgenesforstreptococcussuisinfectioninpigs AT norbertstockhofezurwieden identificationofconditionallyessentialgenesforstreptococcussuisinfectioninpigs AT hildeesmith identificationofconditionallyessentialgenesforstreptococcussuisinfectioninpigs AT astriddegreeff identificationofconditionallyessentialgenesforstreptococcussuisinfectioninpigs
_version_	1718425490046320640

Identification of conditionally essential genes for Streptococcus suis infection in pigs

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