High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics

Monitoring hemodynamics in the brain is important in understanding medical imaging data and mechanisms of disease. Here the authors use high-throughput two-photon microscopy with an axially-extended Bessel focus to measure vessel size and blood flow down to capillary scale in the awake mouse brain.

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Detalles Bibliográficos
Autores principales: Jiang Lan Fan, Jose A. Rivera, Wei Sun, John Peterson, Henry Haeberle, Sam Rubin, Na Ji
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/72d5129c61b241bdb21d6702666d2a38
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Descripción
Sumario:Monitoring hemodynamics in the brain is important in understanding medical imaging data and mechanisms of disease. Here the authors use high-throughput two-photon microscopy with an axially-extended Bessel focus to measure vessel size and blood flow down to capillary scale in the awake mouse brain.