Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6

Upon target RNA recognition, type III CRISPR-Cas systems produce cyclic oligoadenylates that activate effectors such as Csm6 ribonucleases. Here, Garcia-Doval et al. show that Enteroccocus italicus Csm6 degrades its cyclic hexa-AMP activator, and report the crystal structure of the protein bound to...

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Autores principales:	Carmela Garcia-Doval, Frank Schwede, Christian Berk, Jakob T. Rostøl, Ole Niewoehner, Oliver Tejero, Jonathan Hall, Luciano A. Marraffini, Martin Jinek
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/72db17b42bde4a3aab3bcb1de11e229a
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spelling	oai:doaj.org-article:72db17b42bde4a3aab3bcb1de11e229a2021-12-02T14:42:49ZActivation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm610.1038/s41467-020-15334-52041-1723https://doaj.org/article/72db17b42bde4a3aab3bcb1de11e229a2020-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15334-5https://doaj.org/toc/2041-1723Upon target RNA recognition, type III CRISPR-Cas systems produce cyclic oligoadenylates that activate effectors such as Csm6 ribonucleases. Here, Garcia-Doval et al. show that Enteroccocus italicus Csm6 degrades its cyclic hexa-AMP activator, and report the crystal structure of the protein bound to an activator mimic.Carmela Garcia-DovalFrank SchwedeChristian BerkJakob T. RostølOle NiewoehnerOliver TejeroJonathan HallLuciano A. MarraffiniMartin JinekNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Carmela Garcia-Doval Frank Schwede Christian Berk Jakob T. Rostøl Ole Niewoehner Oliver Tejero Jonathan Hall Luciano A. Marraffini Martin Jinek Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6
description	Upon target RNA recognition, type III CRISPR-Cas systems produce cyclic oligoadenylates that activate effectors such as Csm6 ribonucleases. Here, Garcia-Doval et al. show that Enteroccocus italicus Csm6 degrades its cyclic hexa-AMP activator, and report the crystal structure of the protein bound to an activator mimic.
format	article
author	Carmela Garcia-Doval Frank Schwede Christian Berk Jakob T. Rostøl Ole Niewoehner Oliver Tejero Jonathan Hall Luciano A. Marraffini Martin Jinek
author_facet	Carmela Garcia-Doval Frank Schwede Christian Berk Jakob T. Rostøl Ole Niewoehner Oliver Tejero Jonathan Hall Luciano A. Marraffini Martin Jinek
author_sort	Carmela Garcia-Doval
title	Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6
title_short	Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6
title_full	Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6
title_fullStr	Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6
title_full_unstemmed	Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6
title_sort	activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent crispr ribonuclease csm6
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/72db17b42bde4a3aab3bcb1de11e229a
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Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6

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