Detecting genes associated with antimicrobial resistance and pathogen virulence in three New Zealand rivers

The emergence of clinically significant antimicrobial resistance (AMR) in bacteria is frequently attributed to the use of antimicrobials in humans and livestock and is often found concurrently with human and animal pathogens. However, the incidence and natural drivers of antimicrobial resistance and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Meredith Davis, Anne C. Midwinter, Richard Cosgrove, Russell G. Death
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Antimicrobial resistance (AMR) E. coli eDNA Pathogen Water quality Zoonoses Medicine R
Acceso en línea:	https://doaj.org/article/72e9772eb85042ccb72f07e918768846
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:72e9772eb85042ccb72f07e918768846
record_format	dspace
spelling	oai:doaj.org-article:72e9772eb85042ccb72f07e9187688462021-12-05T15:05:06ZDetecting genes associated with antimicrobial resistance and pathogen virulence in three New Zealand rivers10.7717/peerj.124402167-8359https://doaj.org/article/72e9772eb85042ccb72f07e9187688462021-12-01T00:00:00Zhttps://peerj.com/articles/12440.pdfhttps://peerj.com/articles/12440/https://doaj.org/toc/2167-8359The emergence of clinically significant antimicrobial resistance (AMR) in bacteria is frequently attributed to the use of antimicrobials in humans and livestock and is often found concurrently with human and animal pathogens. However, the incidence and natural drivers of antimicrobial resistance and pathogenic virulence in the environment, including waterways and ground water, are poorly understood. Freshwater monitoring for microbial pollution relies on culturing bacterial species indicative of faecal pollution, but detection of genes linked to antimicrobial resistance and/or those linked to virulence is a potentially superior alternative. We collected water and sediment samples in the autumn and spring from three rivers in Canterbury, New Zealand; sites were above and below reaches draining intensive dairy farming. Samples were tested for loci associated with the AMR-related group 1 CTX-M enzyme production (blaCTX-M) and Shiga toxin producing Escherichia coli (STEC). The blaCTX-M locus was only detected during spring and was more prevalent downstream of intensive dairy farms. Loci associated with STEC were detected in both the autumn and spring, again predominantly downstream of intensive dairying. This cross-sectional study suggests that targeted testing of environmental DNA is a useful tool for monitoring waterways. Further studies are now needed to extend our observations across seasons and to examine the relationship between the presence of these genetic elements and the incidence of disease in humans.Meredith DavisAnne C. MidwinterRichard CosgroveRussell G. DeathPeerJ Inc.articleAntimicrobial resistance (AMR)E. colieDNAPathogenWater qualityZoonosesMedicineRENPeerJ, Vol 9, p e12440 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Antimicrobial resistance (AMR) E. coli eDNA Pathogen Water quality Zoonoses Medicine R
spellingShingle	Antimicrobial resistance (AMR) E. coli eDNA Pathogen Water quality Zoonoses Medicine R Meredith Davis Anne C. Midwinter Richard Cosgrove Russell G. Death Detecting genes associated with antimicrobial resistance and pathogen virulence in three New Zealand rivers
description	The emergence of clinically significant antimicrobial resistance (AMR) in bacteria is frequently attributed to the use of antimicrobials in humans and livestock and is often found concurrently with human and animal pathogens. However, the incidence and natural drivers of antimicrobial resistance and pathogenic virulence in the environment, including waterways and ground water, are poorly understood. Freshwater monitoring for microbial pollution relies on culturing bacterial species indicative of faecal pollution, but detection of genes linked to antimicrobial resistance and/or those linked to virulence is a potentially superior alternative. We collected water and sediment samples in the autumn and spring from three rivers in Canterbury, New Zealand; sites were above and below reaches draining intensive dairy farming. Samples were tested for loci associated with the AMR-related group 1 CTX-M enzyme production (blaCTX-M) and Shiga toxin producing Escherichia coli (STEC). The blaCTX-M locus was only detected during spring and was more prevalent downstream of intensive dairy farms. Loci associated with STEC were detected in both the autumn and spring, again predominantly downstream of intensive dairying. This cross-sectional study suggests that targeted testing of environmental DNA is a useful tool for monitoring waterways. Further studies are now needed to extend our observations across seasons and to examine the relationship between the presence of these genetic elements and the incidence of disease in humans.
format	article
author	Meredith Davis Anne C. Midwinter Richard Cosgrove Russell G. Death
author_facet	Meredith Davis Anne C. Midwinter Richard Cosgrove Russell G. Death
author_sort	Meredith Davis
title	Detecting genes associated with antimicrobial resistance and pathogen virulence in three New Zealand rivers
title_short	Detecting genes associated with antimicrobial resistance and pathogen virulence in three New Zealand rivers
title_full	Detecting genes associated with antimicrobial resistance and pathogen virulence in three New Zealand rivers
title_fullStr	Detecting genes associated with antimicrobial resistance and pathogen virulence in three New Zealand rivers
title_full_unstemmed	Detecting genes associated with antimicrobial resistance and pathogen virulence in three New Zealand rivers
title_sort	detecting genes associated with antimicrobial resistance and pathogen virulence in three new zealand rivers
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/72e9772eb85042ccb72f07e918768846
work_keys_str_mv	AT meredithdavis detectinggenesassociatedwithantimicrobialresistanceandpathogenvirulenceinthreenewzealandrivers AT annecmidwinter detectinggenesassociatedwithantimicrobialresistanceandpathogenvirulenceinthreenewzealandrivers AT richardcosgrove detectinggenesassociatedwithantimicrobialresistanceandpathogenvirulenceinthreenewzealandrivers AT russellgdeath detectinggenesassociatedwithantimicrobialresistanceandpathogenvirulenceinthreenewzealandrivers
_version_	1718371305828384768

Detecting genes associated with antimicrobial resistance and pathogen virulence in three New Zealand rivers

Ejemplares similares