Alpha and beta adrenergic receptors modulate keratinocyte migration.

Keratinocyte migration into skin wounds is the step of the healing process that correlates with the wound closure rate. Keratinocyte migration, and wound epithelialization are decreased when beta 2-adrenergic receptors (B2AR) are activated by 1 μM epinephrine/adrenaline, resulting in delayed wound h...

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Autores principales:	Hsin-Ya Yang, Pieter Steenhuis, Aaron M Glucksman, Zhanna Gurenko, Thi Dinh La, R Rivkah Isseroff
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/72ef737cf8754a829755389e52d88a24
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spelling	oai:doaj.org-article:72ef737cf8754a829755389e52d88a242021-12-02T20:05:13ZAlpha and beta adrenergic receptors modulate keratinocyte migration.1932-620310.1371/journal.pone.0253139https://doaj.org/article/72ef737cf8754a829755389e52d88a242021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253139https://doaj.org/toc/1932-6203Keratinocyte migration into skin wounds is the step of the healing process that correlates with the wound closure rate. Keratinocyte migration, and wound epithelialization are decreased when beta 2-adrenergic receptors (B2AR) are activated by 1 μM epinephrine/adrenaline, resulting in delayed wound healing in human and mouse skin. In the present study, we found paradoxically, that in a subset of keratinocyte strains exposure to low concentrations of epinephrine (0.1 nM) increased, rather than decreased, their migratory rate. We find that both the alpha- and the beta-adrenergic receptors are expressed in human keratinocytes, and expression of alpha-2 AR subtypes demonstrated for the first time. Therefore, we tested if the alpha-AR could be modulating the increased migratory response observed in these cell strains. By using specific inhibitors to alpha-AR, we demonstrated that blocking A2B-AR could reverse the rapid cell migration induced by the 0.1 nM epinephrine. Phosphorylation of ERK was elevated after 1-10 minutes of the low epinephrine treatment and the A2B-AR inhibitor blocked the ERK phosphorylation. The results suggest that both the A2B-AR and B2AR mediate keratinocyte migration, in which with a low level of epinephrine treatment, A2B-AR could alter the B2AR signals and regulate the migration rate.Hsin-Ya YangPieter SteenhuisAaron M GlucksmanZhanna GurenkoThi Dinh LaR Rivkah IsseroffPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0253139 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hsin-Ya Yang Pieter Steenhuis Aaron M Glucksman Zhanna Gurenko Thi Dinh La R Rivkah Isseroff Alpha and beta adrenergic receptors modulate keratinocyte migration.
description	Keratinocyte migration into skin wounds is the step of the healing process that correlates with the wound closure rate. Keratinocyte migration, and wound epithelialization are decreased when beta 2-adrenergic receptors (B2AR) are activated by 1 μM epinephrine/adrenaline, resulting in delayed wound healing in human and mouse skin. In the present study, we found paradoxically, that in a subset of keratinocyte strains exposure to low concentrations of epinephrine (0.1 nM) increased, rather than decreased, their migratory rate. We find that both the alpha- and the beta-adrenergic receptors are expressed in human keratinocytes, and expression of alpha-2 AR subtypes demonstrated for the first time. Therefore, we tested if the alpha-AR could be modulating the increased migratory response observed in these cell strains. By using specific inhibitors to alpha-AR, we demonstrated that blocking A2B-AR could reverse the rapid cell migration induced by the 0.1 nM epinephrine. Phosphorylation of ERK was elevated after 1-10 minutes of the low epinephrine treatment and the A2B-AR inhibitor blocked the ERK phosphorylation. The results suggest that both the A2B-AR and B2AR mediate keratinocyte migration, in which with a low level of epinephrine treatment, A2B-AR could alter the B2AR signals and regulate the migration rate.
format	article
author	Hsin-Ya Yang Pieter Steenhuis Aaron M Glucksman Zhanna Gurenko Thi Dinh La R Rivkah Isseroff
author_facet	Hsin-Ya Yang Pieter Steenhuis Aaron M Glucksman Zhanna Gurenko Thi Dinh La R Rivkah Isseroff
author_sort	Hsin-Ya Yang
title	Alpha and beta adrenergic receptors modulate keratinocyte migration.
title_short	Alpha and beta adrenergic receptors modulate keratinocyte migration.
title_full	Alpha and beta adrenergic receptors modulate keratinocyte migration.
title_fullStr	Alpha and beta adrenergic receptors modulate keratinocyte migration.
title_full_unstemmed	Alpha and beta adrenergic receptors modulate keratinocyte migration.
title_sort	alpha and beta adrenergic receptors modulate keratinocyte migration.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/72ef737cf8754a829755389e52d88a24
work_keys_str_mv	AT hsinyayang alphaandbetaadrenergicreceptorsmodulatekeratinocytemigration AT pietersteenhuis alphaandbetaadrenergicreceptorsmodulatekeratinocytemigration AT aaronmglucksman alphaandbetaadrenergicreceptorsmodulatekeratinocytemigration AT zhannagurenko alphaandbetaadrenergicreceptorsmodulatekeratinocytemigration AT thidinhla alphaandbetaadrenergicreceptorsmodulatekeratinocytemigration AT rrivkahisseroff alphaandbetaadrenergicreceptorsmodulatekeratinocytemigration
_version_	1718375488534085632

Alpha and beta adrenergic receptors modulate keratinocyte migration.

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