Cellobiose Consumption Uncouples Extracellular Glucose Sensing and Glucose Metabolism in <italic toggle="yes">Saccharomyces cerevisiae</italic>

ABSTRACT Glycolysis is central to energy metabolism in most organisms and is highly regulated to enable optimal growth. In the yeast Saccharomyces cerevisiae, feedback mechanisms that control flux through glycolysis span transcriptional control to metabolite levels in the cell. Using a cellobiose co...

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Autores principales:	Kulika Chomvong, Daniel I. Benjamin, Daniel K. Nomura, Jamie H. D. Cate
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	PMA1 cellobiose glucose sensors metabolomics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/72f14fd08662418da6611c95a8a13cf4
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spelling	oai:doaj.org-article:72f14fd08662418da6611c95a8a13cf42021-11-15T15:51:43ZCellobiose Consumption Uncouples Extracellular Glucose Sensing and Glucose Metabolism in <italic toggle="yes">Saccharomyces cerevisiae</italic>10.1128/mBio.00855-172150-7511https://doaj.org/article/72f14fd08662418da6611c95a8a13cf42017-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00855-17https://doaj.org/toc/2150-7511ABSTRACT Glycolysis is central to energy metabolism in most organisms and is highly regulated to enable optimal growth. In the yeast Saccharomyces cerevisiae, feedback mechanisms that control flux through glycolysis span transcriptional control to metabolite levels in the cell. Using a cellobiose consumption pathway, we decoupled glucose sensing from carbon utilization, revealing new modular layers of control that induce ATP consumption to drive rapid carbon fermentation. Alterations of the beta subunit of phosphofructokinase-1 (PFK2), H+-plasma membrane ATPase (PMA1), and glucose sensors (SNF3 and RGT2) revealed the importance of coupling extracellular glucose sensing to manage ATP levels in the cell. Controlling the upper bound of cellular ATP levels may be a general mechanism used to regulate energy levels in cells, via a regulatory network that can be uncoupled from ATP concentrations under perceived starvation conditions. IMPORTANCE Living cells are fine-tuned through evolution to thrive in their native environments. Genome alterations to create organisms for specific biotechnological applications may result in unexpected and undesired phenotypes. We used a minimal synthetic biological system in the yeast Saccharomyces cerevisiae as a platform to reveal novel connections between carbon sensing, starvation conditions, and energy homeostasis.Kulika ChomvongDaniel I. BenjaminDaniel K. NomuraJamie H. D. CateAmerican Society for MicrobiologyarticlePMA1cellobioseglucose sensorsmetabolomicsMicrobiologyQR1-502ENmBio, Vol 8, Iss 4 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	PMA1 cellobiose glucose sensors metabolomics Microbiology QR1-502
spellingShingle	PMA1 cellobiose glucose sensors metabolomics Microbiology QR1-502 Kulika Chomvong Daniel I. Benjamin Daniel K. Nomura Jamie H. D. Cate Cellobiose Consumption Uncouples Extracellular Glucose Sensing and Glucose Metabolism in <italic toggle="yes">Saccharomyces cerevisiae</italic>
description	ABSTRACT Glycolysis is central to energy metabolism in most organisms and is highly regulated to enable optimal growth. In the yeast Saccharomyces cerevisiae, feedback mechanisms that control flux through glycolysis span transcriptional control to metabolite levels in the cell. Using a cellobiose consumption pathway, we decoupled glucose sensing from carbon utilization, revealing new modular layers of control that induce ATP consumption to drive rapid carbon fermentation. Alterations of the beta subunit of phosphofructokinase-1 (PFK2), H+-plasma membrane ATPase (PMA1), and glucose sensors (SNF3 and RGT2) revealed the importance of coupling extracellular glucose sensing to manage ATP levels in the cell. Controlling the upper bound of cellular ATP levels may be a general mechanism used to regulate energy levels in cells, via a regulatory network that can be uncoupled from ATP concentrations under perceived starvation conditions. IMPORTANCE Living cells are fine-tuned through evolution to thrive in their native environments. Genome alterations to create organisms for specific biotechnological applications may result in unexpected and undesired phenotypes. We used a minimal synthetic biological system in the yeast Saccharomyces cerevisiae as a platform to reveal novel connections between carbon sensing, starvation conditions, and energy homeostasis.
format	article
author	Kulika Chomvong Daniel I. Benjamin Daniel K. Nomura Jamie H. D. Cate
author_facet	Kulika Chomvong Daniel I. Benjamin Daniel K. Nomura Jamie H. D. Cate
author_sort	Kulika Chomvong
title	Cellobiose Consumption Uncouples Extracellular Glucose Sensing and Glucose Metabolism in <italic toggle="yes">Saccharomyces cerevisiae</italic>
title_short	Cellobiose Consumption Uncouples Extracellular Glucose Sensing and Glucose Metabolism in <italic toggle="yes">Saccharomyces cerevisiae</italic>
title_full	Cellobiose Consumption Uncouples Extracellular Glucose Sensing and Glucose Metabolism in <italic toggle="yes">Saccharomyces cerevisiae</italic>
title_fullStr	Cellobiose Consumption Uncouples Extracellular Glucose Sensing and Glucose Metabolism in <italic toggle="yes">Saccharomyces cerevisiae</italic>
title_full_unstemmed	Cellobiose Consumption Uncouples Extracellular Glucose Sensing and Glucose Metabolism in <italic toggle="yes">Saccharomyces cerevisiae</italic>
title_sort	cellobiose consumption uncouples extracellular glucose sensing and glucose metabolism in <italic toggle="yes">saccharomyces cerevisiae</italic>
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/72f14fd08662418da6611c95a8a13cf4
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Cellobiose Consumption Uncouples Extracellular Glucose Sensing and Glucose Metabolism in <italic toggle="yes">Saccharomyces cerevisiae</italic>

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