IDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODS

We performed clinical observations and laboratory examination of 22 patients with chronic pyelonephritis (chronic renal failure, CRF) and 30 healthy individuals. The patients with CRF were examined twice. The first group (Group I) included patients with exacerbation of the disease. The comparison se...

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Autores principales:	N. B. Zakharova, L. Kh. Pastushkova, R. V. Lakh, I. M. Larina, E. N. Nikolaev, A. N. Ponukalin, N. A. Varaksin, V. I. Оficerov, A. S. Kononikhin
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2019
Materias:	pyelonephritis cytokines urinary biomarkers proteomics mass spectrometry Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/72f4ea6ff7004177bd871d396de4536d
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spelling	oai:doaj.org-article:72f4ea6ff7004177bd871d396de4536d2021-11-18T08:03:48ZIDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODS1563-06252313-741X10.15789/1563-0625-2019-2-341-350https://doaj.org/article/72f4ea6ff7004177bd871d396de4536d2019-05-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1475https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XWe performed clinical observations and laboratory examination of 22 patients with chronic pyelonephritis (chronic renal failure, CRF) and 30 healthy individuals. The patients with CRF were examined twice. The first group (Group I) included patients with exacerbation of the disease. The comparison series (Group II) was represented by the same patients who were examined 1.5-3 months after completion of treatment, without clinical exacerbation of chronic pyelonephritis (CPN). Laboratory signs of acute renal damage were not detectable in all the patients examined. Concentrations of VEGF, MCP-1, IL-8 and IL-18 were determined in urine samples of all examined persons by ELISA technique. Protein spectrum of urine was assessed in six patients from Group I, and in six cases of Group II by means of mass spectrometry, using Agilent 1100 chromatographic device, and LTQ-FT Ultra hybrid mass spectrometer. The results of parallel determination of urine proteins by the two methods have shown that the evolving CPN exacerbation is associated with local secondary immune deficiency at the level of renal tubular urothelium. Determination of urine proteome by means of mass spectrometry in exacerbating disease allows identify the proteins associated with damage to epithelial lining of renal tubules and development of local immune response.N. B. ZakharovaL. Kh. PastushkovaR. V. LakhI. M. LarinaE. N. NikolaevA. N. PonukalinN. A. VaraksinV. I. ОficerovA. S. KononikhinSPb RAACIarticlepyelonephritiscytokinesurinary biomarkersproteomicsmass spectrometryImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 2, Pp 341-350 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	pyelonephritis cytokines urinary biomarkers proteomics mass spectrometry Immunologic diseases. Allergy RC581-607
spellingShingle	pyelonephritis cytokines urinary biomarkers proteomics mass spectrometry Immunologic diseases. Allergy RC581-607 N. B. Zakharova L. Kh. Pastushkova R. V. Lakh I. M. Larina E. N. Nikolaev A. N. Ponukalin N. A. Varaksin V. I. Оficerov A. S. Kononikhin IDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODS
description	We performed clinical observations and laboratory examination of 22 patients with chronic pyelonephritis (chronic renal failure, CRF) and 30 healthy individuals. The patients with CRF were examined twice. The first group (Group I) included patients with exacerbation of the disease. The comparison series (Group II) was represented by the same patients who were examined 1.5-3 months after completion of treatment, without clinical exacerbation of chronic pyelonephritis (CPN). Laboratory signs of acute renal damage were not detectable in all the patients examined. Concentrations of VEGF, MCP-1, IL-8 and IL-18 were determined in urine samples of all examined persons by ELISA technique. Protein spectrum of urine was assessed in six patients from Group I, and in six cases of Group II by means of mass spectrometry, using Agilent 1100 chromatographic device, and LTQ-FT Ultra hybrid mass spectrometer. The results of parallel determination of urine proteins by the two methods have shown that the evolving CPN exacerbation is associated with local secondary immune deficiency at the level of renal tubular urothelium. Determination of urine proteome by means of mass spectrometry in exacerbating disease allows identify the proteins associated with damage to epithelial lining of renal tubules and development of local immune response.
format	article
author	N. B. Zakharova L. Kh. Pastushkova R. V. Lakh I. M. Larina E. N. Nikolaev A. N. Ponukalin N. A. Varaksin V. I. Оficerov A. S. Kononikhin
author_facet	N. B. Zakharova L. Kh. Pastushkova R. V. Lakh I. M. Larina E. N. Nikolaev A. N. Ponukalin N. A. Varaksin V. I. Оficerov A. S. Kononikhin
author_sort	N. B. Zakharova
title	IDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODS
title_short	IDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODS
title_full	IDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODS
title_fullStr	IDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODS
title_full_unstemmed	IDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODS
title_sort	identification of biomarkers of renal parenchyma damage in the urine of patients with chronic pyelonephritis by elisa and mass spectrometry methods
publisher	SPb RAACI
publishDate	2019
url	https://doaj.org/article/72f4ea6ff7004177bd871d396de4536d
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IDENTIFICATION OF BIOMARKERS OF RENAL PARENCHYMA DAMAGE IN THE URINE OF PATIENTS WITH CHRONIC PYELONEPHRITIS BY ELISA AND MASS SPECTROMETRY METHODS

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