Advantageous environment of micro-patterned, high-density complementary metal–oxide–semiconductor electrode array for spiral ganglion neurons cultured in vitro

Abstract This study investigated micro-patterned, high-density complementary metal–oxide–semiconductor (CMOS) electrode array to be used as biologically permissive environment for organization, guidance and electrical stimulation of spiral ganglion neurons (SGN). SGNs extracted and isolated from coc...

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Autores principales:	Viktorija Radotić, Dries Braeken, Petar Drviš, Marta Mattotti, Damir Kovačić
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/72fca3cd0fb245e4a6037075e767ec1e
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spelling	oai:doaj.org-article:72fca3cd0fb245e4a6037075e767ec1e2021-12-02T11:41:03ZAdvantageous environment of micro-patterned, high-density complementary metal–oxide–semiconductor electrode array for spiral ganglion neurons cultured in vitro10.1038/s41598-018-25814-w2045-2322https://doaj.org/article/72fca3cd0fb245e4a6037075e767ec1e2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25814-whttps://doaj.org/toc/2045-2322Abstract This study investigated micro-patterned, high-density complementary metal–oxide–semiconductor (CMOS) electrode array to be used as biologically permissive environment for organization, guidance and electrical stimulation of spiral ganglion neurons (SGN). SGNs extracted and isolated from cochleae of P5-P7 rat pups and adult guinea pigs were cultured 1, 4 and 7 days in vitro on glass coverslips (control) and CMOS electrode array. The cultures were analyzed visually and immunohistochemically for SGN presence, outgrowth, neurite alignment, neurite length, neurite asymmetry as well as the contact of a neuronal soma and neurites with the micro-electrodes. Our findings indicate that topographical environment of CMOS chip with micro-patterned pillars enhanced growth, survival, morphology, neural orientation and alignment of SGNs in vitro compared to control. Smaller spacing (0.8–1.6 µm) between protruding pillars on CMOS led SGNs to develop structured and guided neurites oriented along three topographical axes separated by 60°. We found morphological basis for positioning of the micro-electrodes on the chip that was appropriate for direct contact of SGNs with them. This configuration allowed CMOS electrode array to electrically stimulate the SGN whose responses were observed with live Fluo 4 calcium imaging.Viktorija RadotićDries BraekenPetar DrvišMarta MattottiDamir KovačićNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-19 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Viktorija Radotić Dries Braeken Petar Drviš Marta Mattotti Damir Kovačić Advantageous environment of micro-patterned, high-density complementary metal–oxide–semiconductor electrode array for spiral ganglion neurons cultured in vitro
description	Abstract This study investigated micro-patterned, high-density complementary metal–oxide–semiconductor (CMOS) electrode array to be used as biologically permissive environment for organization, guidance and electrical stimulation of spiral ganglion neurons (SGN). SGNs extracted and isolated from cochleae of P5-P7 rat pups and adult guinea pigs were cultured 1, 4 and 7 days in vitro on glass coverslips (control) and CMOS electrode array. The cultures were analyzed visually and immunohistochemically for SGN presence, outgrowth, neurite alignment, neurite length, neurite asymmetry as well as the contact of a neuronal soma and neurites with the micro-electrodes. Our findings indicate that topographical environment of CMOS chip with micro-patterned pillars enhanced growth, survival, morphology, neural orientation and alignment of SGNs in vitro compared to control. Smaller spacing (0.8–1.6 µm) between protruding pillars on CMOS led SGNs to develop structured and guided neurites oriented along three topographical axes separated by 60°. We found morphological basis for positioning of the micro-electrodes on the chip that was appropriate for direct contact of SGNs with them. This configuration allowed CMOS electrode array to electrically stimulate the SGN whose responses were observed with live Fluo 4 calcium imaging.
format	article
author	Viktorija Radotić Dries Braeken Petar Drviš Marta Mattotti Damir Kovačić
author_facet	Viktorija Radotić Dries Braeken Petar Drviš Marta Mattotti Damir Kovačić
author_sort	Viktorija Radotić
title	Advantageous environment of micro-patterned, high-density complementary metal–oxide–semiconductor electrode array for spiral ganglion neurons cultured in vitro
title_short	Advantageous environment of micro-patterned, high-density complementary metal–oxide–semiconductor electrode array for spiral ganglion neurons cultured in vitro
title_full	Advantageous environment of micro-patterned, high-density complementary metal–oxide–semiconductor electrode array for spiral ganglion neurons cultured in vitro
title_fullStr	Advantageous environment of micro-patterned, high-density complementary metal–oxide–semiconductor electrode array for spiral ganglion neurons cultured in vitro
title_full_unstemmed	Advantageous environment of micro-patterned, high-density complementary metal–oxide–semiconductor electrode array for spiral ganglion neurons cultured in vitro
title_sort	advantageous environment of micro-patterned, high-density complementary metal–oxide–semiconductor electrode array for spiral ganglion neurons cultured in vitro
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/72fca3cd0fb245e4a6037075e767ec1e
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Advantageous environment of micro-patterned, high-density complementary metal–oxide–semiconductor electrode array for spiral ganglion neurons cultured in vitro

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