Evaluation of ocular surface glycocalyx using lectin-conjugated fluorescein

Hiroshi Mochizuki1, Masaki Fukui1,2, Shin Hatou1,2, Masakazu Yamada1, Kazuo Tsubota21Division for Vision Research, National Institute of Sensory Organs, National Tokyo Medical Center, Tokyo, Japan; 2Department of Ophthalmology, Keio University School of Medicine, Tokyo, JapanPurpose: A glycocalyx pl...

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Autores principales:	Hiroshi Mochizuki, Masaki Fukui, Shin Hatou, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/72ff42666c194f4f9e95f614d3a26075
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spelling	oai:doaj.org-article:72ff42666c194f4f9e95f614d3a260752021-12-02T00:51:06ZEvaluation of ocular surface glycocalyx using lectin-conjugated fluorescein1177-54671177-5483https://doaj.org/article/72ff42666c194f4f9e95f614d3a260752010-08-01T00:00:00Zhttp://www.dovepress.com/evaluation-of-ocular-surface-glycocalyx-using-lectin-conjugated-fluore-a5059https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Hiroshi Mochizuki1, Masaki Fukui1,2, Shin Hatou1,2, Masakazu Yamada1, Kazuo Tsubota21Division for Vision Research, National Institute of Sensory Organs, National Tokyo Medical Center, Tokyo, Japan; 2Department of Ophthalmology, Keio University School of Medicine, Tokyo, JapanPurpose: A glycocalyx plays important roles in the ocular surface epithelium, but there is no direct simple method to evaluate ocular surface glycocalyx. We tested a wheat germ agglutinin conjugate of fluorescein (F-WGA) as a marker to demonstrate ocular surface glycocalyx in vivo.Methods: After a 5% F-WGA solution was applied to the eyes of eight healthy volunteers, fluorescent intensities of the central cornea and bulbar conjunctiva were measured by fluorophotometry. A 10% fluorescein-conjugated dextran solution served as the control. Changes in fluorescent intensities of the ocular surface following a challenge with 5% N-acetyl cysteine, a conventional mucolytic agent, were tested in the second experiment. Saline instillation served as a control.Results: The ocular surface was diffusely stained by F-WGA. Breakup of the precorneal tear film was not apparent, possibly because F-WGA was bound to the glycocalyx of the ocular surface epithelium. F-WGA fluorescent intensities were high in the superior, nasal, and inferior regions of the bulbar conjunctiva and low in the temporal conjunctiva and cornea. No such regional differences were observed with fluorescein-conjugated dextran. F-WGA fluorescent intensities decreased significantly with N-acetyl cysteine instillation, whereas they were not affected by saline instillation.Conclusion: The fluorophotometric method may be used to evaluate the glycocalyx quantitatively in the ocular surface in vivo.Keywords: cornea, fluorescein, glycocalyx, lectin, mucin Hiroshi MochizukiMasaki FukuiShin Hatouet alDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2010, Iss default, Pp 925-930 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Hiroshi Mochizuki Masaki Fukui Shin Hatou et al Evaluation of ocular surface glycocalyx using lectin-conjugated fluorescein
description	Hiroshi Mochizuki1, Masaki Fukui1,2, Shin Hatou1,2, Masakazu Yamada1, Kazuo Tsubota21Division for Vision Research, National Institute of Sensory Organs, National Tokyo Medical Center, Tokyo, Japan; 2Department of Ophthalmology, Keio University School of Medicine, Tokyo, JapanPurpose: A glycocalyx plays important roles in the ocular surface epithelium, but there is no direct simple method to evaluate ocular surface glycocalyx. We tested a wheat germ agglutinin conjugate of fluorescein (F-WGA) as a marker to demonstrate ocular surface glycocalyx in vivo.Methods: After a 5% F-WGA solution was applied to the eyes of eight healthy volunteers, fluorescent intensities of the central cornea and bulbar conjunctiva were measured by fluorophotometry. A 10% fluorescein-conjugated dextran solution served as the control. Changes in fluorescent intensities of the ocular surface following a challenge with 5% N-acetyl cysteine, a conventional mucolytic agent, were tested in the second experiment. Saline instillation served as a control.Results: The ocular surface was diffusely stained by F-WGA. Breakup of the precorneal tear film was not apparent, possibly because F-WGA was bound to the glycocalyx of the ocular surface epithelium. F-WGA fluorescent intensities were high in the superior, nasal, and inferior regions of the bulbar conjunctiva and low in the temporal conjunctiva and cornea. No such regional differences were observed with fluorescein-conjugated dextran. F-WGA fluorescent intensities decreased significantly with N-acetyl cysteine instillation, whereas they were not affected by saline instillation.Conclusion: The fluorophotometric method may be used to evaluate the glycocalyx quantitatively in the ocular surface in vivo.Keywords: cornea, fluorescein, glycocalyx, lectin, mucin
format	article
author	Hiroshi Mochizuki Masaki Fukui Shin Hatou et al
author_facet	Hiroshi Mochizuki Masaki Fukui Shin Hatou et al
author_sort	Hiroshi Mochizuki
title	Evaluation of ocular surface glycocalyx using lectin-conjugated fluorescein
title_short	Evaluation of ocular surface glycocalyx using lectin-conjugated fluorescein
title_full	Evaluation of ocular surface glycocalyx using lectin-conjugated fluorescein
title_fullStr	Evaluation of ocular surface glycocalyx using lectin-conjugated fluorescein
title_full_unstemmed	Evaluation of ocular surface glycocalyx using lectin-conjugated fluorescein
title_sort	evaluation of ocular surface glycocalyx using lectin-conjugated fluorescein
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/72ff42666c194f4f9e95f614d3a26075
work_keys_str_mv	AT hiroshimochizuki evaluationofocularsurfaceglycocalyxusinglectinconjugatedfluorescein AT masakifukui evaluationofocularsurfaceglycocalyxusinglectinconjugatedfluorescein AT shinhatou evaluationofocularsurfaceglycocalyxusinglectinconjugatedfluorescein AT etal evaluationofocularsurfaceglycocalyxusinglectinconjugatedfluorescein
_version_	1718403446150791168

Evaluation of ocular surface glycocalyx using lectin-conjugated fluorescein

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