Structural characterization and protective effects of polysaccharide from Gracilaria lemaneiformis on LPS-induced injury in IEC-6 cells

This study was aimed to characterize Gracilaria lemaneiformis polysaccharides and evaluate their protective effects on Lipopolysaccharide-induced injury in IEC-6 cells. The G. lemaneiformis polysaccharide was degraded by UV/H2O2 treatment and purified to three fractions named GLP-1.0 M, GLP-1.4 M an...

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Autores principales:	Xiong Li, Yufeng Gong, Wanzi Yao, Xiaoyong Chen, Jiebei Xian, Lijun You, Pedro Fardim
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Gracilaria lemaneiformis Sulfated polysaccharide Anti-inflammatory activity Tight junction Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
Acceso en línea:	https://doaj.org/article/730a1a273f8a461198a68ba90be88266
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spelling	oai:doaj.org-article:730a1a273f8a461198a68ba90be882662021-11-14T04:35:25ZStructural characterization and protective effects of polysaccharide from Gracilaria lemaneiformis on LPS-induced injury in IEC-6 cells2590-157510.1016/j.fochx.2021.100157https://doaj.org/article/730a1a273f8a461198a68ba90be882662021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2590157521000456https://doaj.org/toc/2590-1575This study was aimed to characterize Gracilaria lemaneiformis polysaccharides and evaluate their protective effects on Lipopolysaccharide-induced injury in IEC-6 cells. The G. lemaneiformis polysaccharide was degraded by UV/H2O2 treatment and purified to three fractions named GLP-1.0 M, GLP-1.4 M and GLP-1.6 M. The purified fractions were mainly composed of galactose, glucose and xylose. The structural analysis showed that GLP-1.6 M was a typical sulfated red alga polysaccharide containing the linear backbone of β-(1 → 3)- and α-(1 → 4)-linked galactosyl residues, anhydro-galactose units. In the Lipopolysaccharide-induced IEC-6 cells model, GLP-1.6 M exerted the strongest in vitro anti-inflammatory activity by inhibiting the release and expressions of tumor necrosis factor-α, interleukin-6 and interleukin-1β by 89.93%, 67.82% and 38.06%, respectively. Meanwhile, GLP-1.6 M enhanced the intestinal barrier function via up-regulating the expressions of tight junctions and mucin. Therefore, the purified polysaccharide from G. lemaneiformis could be a promising candidate for maintaining intestinal health in the food and pharmaceutical industries.Xiong LiYufeng GongWanzi YaoXiaoyong ChenJiebei XianLijun YouPedro FardimElsevierarticleGracilaria lemaneiformisSulfated polysaccharideAnti-inflammatory activityTight junctionNutrition. Foods and food supplyTX341-641Food processing and manufactureTP368-456ENFood Chemistry: X, Vol 12, Iss , Pp 100157- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gracilaria lemaneiformis Sulfated polysaccharide Anti-inflammatory activity Tight junction Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
spellingShingle	Gracilaria lemaneiformis Sulfated polysaccharide Anti-inflammatory activity Tight junction Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 Xiong Li Yufeng Gong Wanzi Yao Xiaoyong Chen Jiebei Xian Lijun You Pedro Fardim Structural characterization and protective effects of polysaccharide from Gracilaria lemaneiformis on LPS-induced injury in IEC-6 cells
description	This study was aimed to characterize Gracilaria lemaneiformis polysaccharides and evaluate their protective effects on Lipopolysaccharide-induced injury in IEC-6 cells. The G. lemaneiformis polysaccharide was degraded by UV/H2O2 treatment and purified to three fractions named GLP-1.0 M, GLP-1.4 M and GLP-1.6 M. The purified fractions were mainly composed of galactose, glucose and xylose. The structural analysis showed that GLP-1.6 M was a typical sulfated red alga polysaccharide containing the linear backbone of β-(1 → 3)- and α-(1 → 4)-linked galactosyl residues, anhydro-galactose units. In the Lipopolysaccharide-induced IEC-6 cells model, GLP-1.6 M exerted the strongest in vitro anti-inflammatory activity by inhibiting the release and expressions of tumor necrosis factor-α, interleukin-6 and interleukin-1β by 89.93%, 67.82% and 38.06%, respectively. Meanwhile, GLP-1.6 M enhanced the intestinal barrier function via up-regulating the expressions of tight junctions and mucin. Therefore, the purified polysaccharide from G. lemaneiformis could be a promising candidate for maintaining intestinal health in the food and pharmaceutical industries.
format	article
author	Xiong Li Yufeng Gong Wanzi Yao Xiaoyong Chen Jiebei Xian Lijun You Pedro Fardim
author_facet	Xiong Li Yufeng Gong Wanzi Yao Xiaoyong Chen Jiebei Xian Lijun You Pedro Fardim
author_sort	Xiong Li
title	Structural characterization and protective effects of polysaccharide from Gracilaria lemaneiformis on LPS-induced injury in IEC-6 cells
title_short	Structural characterization and protective effects of polysaccharide from Gracilaria lemaneiformis on LPS-induced injury in IEC-6 cells
title_full	Structural characterization and protective effects of polysaccharide from Gracilaria lemaneiformis on LPS-induced injury in IEC-6 cells
title_fullStr	Structural characterization and protective effects of polysaccharide from Gracilaria lemaneiformis on LPS-induced injury in IEC-6 cells
title_full_unstemmed	Structural characterization and protective effects of polysaccharide from Gracilaria lemaneiformis on LPS-induced injury in IEC-6 cells
title_sort	structural characterization and protective effects of polysaccharide from gracilaria lemaneiformis on lps-induced injury in iec-6 cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/730a1a273f8a461198a68ba90be88266
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Structural characterization and protective effects of polysaccharide from Gracilaria lemaneiformis on LPS-induced injury in IEC-6 cells

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