Blood Nanoparticles – Influence on Extracellular Vesicle Isolation and Characterization

Blood is a rich source of disease biomarkers, which include extracellular vesicles (EVs). EVs are nanometer-to micrometer-sized spherical particles that are enclosed by a phospholipid bilayer and are secreted by most cell types. EVs reflect the physiological cell of origin in terms of their molecula...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marija Holcar, Maša Kandušer, Metka Lenassi
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	extracellular vesicles blood nanoparticles contaminants isolation methods characterization methods Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/73168fe074a64d1c8dec9d781d573eec
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:73168fe074a64d1c8dec9d781d573eec
record_format	dspace
spelling	oai:doaj.org-article:73168fe074a64d1c8dec9d781d573eec2021-11-10T16:08:05ZBlood Nanoparticles – Influence on Extracellular Vesicle Isolation and Characterization1663-981210.3389/fphar.2021.773844https://doaj.org/article/73168fe074a64d1c8dec9d781d573eec2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.773844/fullhttps://doaj.org/toc/1663-9812Blood is a rich source of disease biomarkers, which include extracellular vesicles (EVs). EVs are nanometer-to micrometer-sized spherical particles that are enclosed by a phospholipid bilayer and are secreted by most cell types. EVs reflect the physiological cell of origin in terms of their molecular composition and biophysical characteristics, and they accumulate in blood even when released from remote organs or tissues, while protecting their cargo from degradation. The molecular components (e.g., proteins, miRNAs) and biophysical characteristics (e.g., size, concentration) of blood EVs have been studied as biomarkers of cancers and neurodegenerative, autoimmune, and cardiovascular diseases. However, most biomarker studies do not address the problem of contaminants in EV isolates from blood plasma, and how these might affect downstream EV analysis. Indeed, nonphysiological EVs, protein aggregates, lipoproteins and viruses share many molecular and/or biophysical characteristics with EVs, and can therefore co-isolate with EVs from blood plasma. Consequently, isolation and downstream analysis of EVs from blood plasma remain a unique challenge, with important impacts on the outcomes of biomarker studies. To help improve rigor, reproducibility, and reliability of EV biomarker studies, we describe here the major contaminants of EV isolates from blood plasma, and we report on how different EV isolation methods affect their levels, and how contaminants that remain can affect the interpretation of downstream EV analysis.Marija HolcarMaša KandušerMetka LenassiFrontiers Media S.A.articleextracellular vesiclesblood nanoparticlescontaminantsisolation methodscharacterization methodsTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	extracellular vesicles blood nanoparticles contaminants isolation methods characterization methods Therapeutics. Pharmacology RM1-950
spellingShingle	extracellular vesicles blood nanoparticles contaminants isolation methods characterization methods Therapeutics. Pharmacology RM1-950 Marija Holcar Maša Kandušer Metka Lenassi Blood Nanoparticles – Influence on Extracellular Vesicle Isolation and Characterization
description	Blood is a rich source of disease biomarkers, which include extracellular vesicles (EVs). EVs are nanometer-to micrometer-sized spherical particles that are enclosed by a phospholipid bilayer and are secreted by most cell types. EVs reflect the physiological cell of origin in terms of their molecular composition and biophysical characteristics, and they accumulate in blood even when released from remote organs or tissues, while protecting their cargo from degradation. The molecular components (e.g., proteins, miRNAs) and biophysical characteristics (e.g., size, concentration) of blood EVs have been studied as biomarkers of cancers and neurodegenerative, autoimmune, and cardiovascular diseases. However, most biomarker studies do not address the problem of contaminants in EV isolates from blood plasma, and how these might affect downstream EV analysis. Indeed, nonphysiological EVs, protein aggregates, lipoproteins and viruses share many molecular and/or biophysical characteristics with EVs, and can therefore co-isolate with EVs from blood plasma. Consequently, isolation and downstream analysis of EVs from blood plasma remain a unique challenge, with important impacts on the outcomes of biomarker studies. To help improve rigor, reproducibility, and reliability of EV biomarker studies, we describe here the major contaminants of EV isolates from blood plasma, and we report on how different EV isolation methods affect their levels, and how contaminants that remain can affect the interpretation of downstream EV analysis.
format	article
author	Marija Holcar Maša Kandušer Metka Lenassi
author_facet	Marija Holcar Maša Kandušer Metka Lenassi
author_sort	Marija Holcar
title	Blood Nanoparticles – Influence on Extracellular Vesicle Isolation and Characterization
title_short	Blood Nanoparticles – Influence on Extracellular Vesicle Isolation and Characterization
title_full	Blood Nanoparticles – Influence on Extracellular Vesicle Isolation and Characterization
title_fullStr	Blood Nanoparticles – Influence on Extracellular Vesicle Isolation and Characterization
title_full_unstemmed	Blood Nanoparticles – Influence on Extracellular Vesicle Isolation and Characterization
title_sort	blood nanoparticles – influence on extracellular vesicle isolation and characterization
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/73168fe074a64d1c8dec9d781d573eec
work_keys_str_mv	AT marijaholcar bloodnanoparticlesinfluenceonextracellularvesicleisolationandcharacterization AT masakanduser bloodnanoparticlesinfluenceonextracellularvesicleisolationandcharacterization AT metkalenassi bloodnanoparticlesinfluenceonextracellularvesicleisolationandcharacterization
_version_	1718439879636942848

Blood Nanoparticles – Influence on Extracellular Vesicle Isolation and Characterization

Ejemplares similares