Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction

Epigenetic information is transmitted from mother to daughter cells through mitosis. Here, the authors isolate native chromosomes from metaphase-arrested cells and perform LC-MS/MS to identify chromosome-bound proteins in pluripotent stem cells during mitosis and reveal that PRC2, DNA methylation an...

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Autores principales:	Dounia Djeghloul, Bhavik Patel, Holger Kramer, Andrew Dimond, Chad Whilding, Karen Brown, Anne-Céline Kohler, Amelie Feytout, Nicolas Veland, James Elliott, Tanmay A. M. Bharat, Abul K. Tarafder, Jan Löwe, Bee L. Ng, Ya Guo, Jacky Guy, Miles K. Huseyin, Robert J. Klose, Matthias Merkenschlager, Amanda G. Fisher
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/73176d0dba7348dcb968ea5de1aa87c6
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spelling	oai:doaj.org-article:73176d0dba7348dcb968ea5de1aa87c62021-12-02T18:01:15ZIdentifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction10.1038/s41467-020-17823-z2041-1723https://doaj.org/article/73176d0dba7348dcb968ea5de1aa87c62020-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17823-zhttps://doaj.org/toc/2041-1723Epigenetic information is transmitted from mother to daughter cells through mitosis. Here, the authors isolate native chromosomes from metaphase-arrested cells and perform LC-MS/MS to identify chromosome-bound proteins in pluripotent stem cells during mitosis and reveal that PRC2, DNA methylation and Mecp2 are required to maintain chromosome compaction.Dounia DjeghloulBhavik PatelHolger KramerAndrew DimondChad WhildingKaren BrownAnne-Céline KohlerAmelie FeytoutNicolas VelandJames ElliottTanmay A. M. BharatAbul K. TarafderJan LöweBee L. NgYa GuoJacky GuyMiles K. HuseyinRobert J. KloseMatthias MerkenschlagerAmanda G. FisherNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Dounia Djeghloul Bhavik Patel Holger Kramer Andrew Dimond Chad Whilding Karen Brown Anne-Céline Kohler Amelie Feytout Nicolas Veland James Elliott Tanmay A. M. Bharat Abul K. Tarafder Jan Löwe Bee L. Ng Ya Guo Jacky Guy Miles K. Huseyin Robert J. Klose Matthias Merkenschlager Amanda G. Fisher Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction
description	Epigenetic information is transmitted from mother to daughter cells through mitosis. Here, the authors isolate native chromosomes from metaphase-arrested cells and perform LC-MS/MS to identify chromosome-bound proteins in pluripotent stem cells during mitosis and reveal that PRC2, DNA methylation and Mecp2 are required to maintain chromosome compaction.
format	article
author	Dounia Djeghloul Bhavik Patel Holger Kramer Andrew Dimond Chad Whilding Karen Brown Anne-Céline Kohler Amelie Feytout Nicolas Veland James Elliott Tanmay A. M. Bharat Abul K. Tarafder Jan Löwe Bee L. Ng Ya Guo Jacky Guy Miles K. Huseyin Robert J. Klose Matthias Merkenschlager Amanda G. Fisher
author_facet	Dounia Djeghloul Bhavik Patel Holger Kramer Andrew Dimond Chad Whilding Karen Brown Anne-Céline Kohler Amelie Feytout Nicolas Veland James Elliott Tanmay A. M. Bharat Abul K. Tarafder Jan Löwe Bee L. Ng Ya Guo Jacky Guy Miles K. Huseyin Robert J. Klose Matthias Merkenschlager Amanda G. Fisher
author_sort	Dounia Djeghloul
title	Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction
title_short	Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction
title_full	Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction
title_fullStr	Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction
title_full_unstemmed	Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction
title_sort	identifying proteins bound to native mitotic esc chromosomes reveals chromatin repressors are important for compaction
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/73176d0dba7348dcb968ea5de1aa87c6
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Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction

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