Cisatracurium besilate enhances the TRAIL-induced apoptosis of gastric cancer cells via p53 signaling

Cisatracurium besilate is the most commonly used non-depolarizing muscle relaxant in general anesthesia and in intensive care units. Studies have indicated that the proliferation of gastric cancer (GC) cells can be restrained by cisatracurium besilate. The present study aimed to investigate the mech...

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Autores principales:	Qiang Zhou, Jianxia Yuan, Yi Liu, Yayun Wu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	cisatracurium besilate gastric cancer tnf-related apoptosis-inducing ligand apoptosis Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/73257cfb3b5740bb9f9a68a5c1e86176
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spelling	oai:doaj.org-article:73257cfb3b5740bb9f9a68a5c1e861762021-12-01T14:41:00ZCisatracurium besilate enhances the TRAIL-induced apoptosis of gastric cancer cells via p53 signaling2165-59792165-598710.1080/21655979.2021.2009318https://doaj.org/article/73257cfb3b5740bb9f9a68a5c1e861762021-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2009318https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Cisatracurium besilate is the most commonly used non-depolarizing muscle relaxant in general anesthesia and in intensive care units. Studies have indicated that the proliferation of gastric cancer (GC) cells can be restrained by cisatracurium besilate. The present study aimed to investigate the mechanism underlying the role of cisatracurium besilate in TNF-related apoptosis-inducing ligand (TRAIL)-induced GC. The AGS cell line was exposed to cisatracurium besilate, and then cell viability, colony formation and apoptosis were assessed by performing Cell Counting Kit-8, colony formation, TUNEL and Western blot assays, respectively. Furthermore, the expression levels of p53 and p53 upregulated modulator of apoptosis (PUMA) were measured by Western blotting to determine the effect of cisatracurium besilate on p53/PUMA signaling. After co-treatment with p53 inhibitor, cisatracurium besilate and pifithrin-α/TRAIL, cell apoptosis was detected. Finally, cisatracurium besilate and pifithrin-α were used to co-treat TRAIL-induced AGS cells followed by apoptosis detection. Cisatracurium besilate treatment restrained the proliferation and promoted the apoptosis of AGS cells. Cisatracurium besilate also promoted the expression of p53 and PUMA in AGS cells. Furthermore, TRAIL induced the apoptosis of AGS cells, which was aggravated by cisatracurium besilate treatment. However, pifithrin-α reversed the synergistic effects of cisatracurium besilate and TRAIL on the activities of AGS cells. Therefore, the present study suggested that cisatracurium besilate enhanced the TRAIL-induced apoptosis of GC cells via p53 signaling, and the synergistic effects of cisatracurium besilate and TRAIL may achieve maximal therapeutic efficacy in GC management.Qiang ZhouJianxia YuanYi LiuYayun WuTaylor & Francis Grouparticlecisatracurium besilategastric cancertnf-related apoptosis-inducing ligandapoptosisBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 2, Pp 11213-11224 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cisatracurium besilate gastric cancer tnf-related apoptosis-inducing ligand apoptosis Biotechnology TP248.13-248.65
spellingShingle	cisatracurium besilate gastric cancer tnf-related apoptosis-inducing ligand apoptosis Biotechnology TP248.13-248.65 Qiang Zhou Jianxia Yuan Yi Liu Yayun Wu Cisatracurium besilate enhances the TRAIL-induced apoptosis of gastric cancer cells via p53 signaling
description	Cisatracurium besilate is the most commonly used non-depolarizing muscle relaxant in general anesthesia and in intensive care units. Studies have indicated that the proliferation of gastric cancer (GC) cells can be restrained by cisatracurium besilate. The present study aimed to investigate the mechanism underlying the role of cisatracurium besilate in TNF-related apoptosis-inducing ligand (TRAIL)-induced GC. The AGS cell line was exposed to cisatracurium besilate, and then cell viability, colony formation and apoptosis were assessed by performing Cell Counting Kit-8, colony formation, TUNEL and Western blot assays, respectively. Furthermore, the expression levels of p53 and p53 upregulated modulator of apoptosis (PUMA) were measured by Western blotting to determine the effect of cisatracurium besilate on p53/PUMA signaling. After co-treatment with p53 inhibitor, cisatracurium besilate and pifithrin-α/TRAIL, cell apoptosis was detected. Finally, cisatracurium besilate and pifithrin-α were used to co-treat TRAIL-induced AGS cells followed by apoptosis detection. Cisatracurium besilate treatment restrained the proliferation and promoted the apoptosis of AGS cells. Cisatracurium besilate also promoted the expression of p53 and PUMA in AGS cells. Furthermore, TRAIL induced the apoptosis of AGS cells, which was aggravated by cisatracurium besilate treatment. However, pifithrin-α reversed the synergistic effects of cisatracurium besilate and TRAIL on the activities of AGS cells. Therefore, the present study suggested that cisatracurium besilate enhanced the TRAIL-induced apoptosis of GC cells via p53 signaling, and the synergistic effects of cisatracurium besilate and TRAIL may achieve maximal therapeutic efficacy in GC management.
format	article
author	Qiang Zhou Jianxia Yuan Yi Liu Yayun Wu
author_facet	Qiang Zhou Jianxia Yuan Yi Liu Yayun Wu
author_sort	Qiang Zhou
title	Cisatracurium besilate enhances the TRAIL-induced apoptosis of gastric cancer cells via p53 signaling
title_short	Cisatracurium besilate enhances the TRAIL-induced apoptosis of gastric cancer cells via p53 signaling
title_full	Cisatracurium besilate enhances the TRAIL-induced apoptosis of gastric cancer cells via p53 signaling
title_fullStr	Cisatracurium besilate enhances the TRAIL-induced apoptosis of gastric cancer cells via p53 signaling
title_full_unstemmed	Cisatracurium besilate enhances the TRAIL-induced apoptosis of gastric cancer cells via p53 signaling
title_sort	cisatracurium besilate enhances the trail-induced apoptosis of gastric cancer cells via p53 signaling
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/73257cfb3b5740bb9f9a68a5c1e86176
work_keys_str_mv	AT qiangzhou cisatracuriumbesilateenhancesthetrailinducedapoptosisofgastriccancercellsviap53signaling AT jianxiayuan cisatracuriumbesilateenhancesthetrailinducedapoptosisofgastriccancercellsviap53signaling AT yiliu cisatracuriumbesilateenhancesthetrailinducedapoptosisofgastriccancercellsviap53signaling AT yayunwu cisatracuriumbesilateenhancesthetrailinducedapoptosisofgastriccancercellsviap53signaling
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Cisatracurium besilate enhances the TRAIL-induced apoptosis of gastric cancer cells via p53 signaling

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