A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics

The objective of this study was to evaluate the antibacterial mechanisms of phenolic acids as natural approaches against multi-drug resistant Escherichia coli (E. coli). For that purpose, five phenolic acids were combined with each other and 31 combinations were obtained in total. To select the most...

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Autores principales:	Geyin Zhang, Yunqiao Yang, Fareed Uddin Memon, Kaiyuan Hao, Baichang Xu, Shuaiyang Wang, Ying Wang, Enyun Wu, Xiaogang Chen, Wenguang Xiong, Hongbin Si
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	compound phenolic acids Escherichia coli TMT antibacterial mechanism new antimicrobial agent Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/732e23e0f1d747b8b5f213f7fc70e661
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spelling	oai:doaj.org-article:732e23e0f1d747b8b5f213f7fc70e6612021-12-01T13:16:51ZA Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics1664-302X10.3389/fmicb.2021.738896https://doaj.org/article/732e23e0f1d747b8b5f213f7fc70e6612021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.738896/fullhttps://doaj.org/toc/1664-302XThe objective of this study was to evaluate the antibacterial mechanisms of phenolic acids as natural approaches against multi-drug resistant Escherichia coli (E. coli). For that purpose, five phenolic acids were combined with each other and 31 combinations were obtained in total. To select the most potent and effective combination, all of the obtained combinations were examined for minimum inhibitory concentration (MIC) and it was found that the compound phenolic acid (CPA) 19 (protocatechuic acid, hydrocinnamic acid, and chlorogenic acid at concentrations of 0.833, 0.208, and 1.677 mg/mL, respectively) showed better efficacy against E. coli compared to other combinations. Furthermore, based on tandem mass tag (TMT) proteomics, the treatment of CPA 19 significantly downregulated the proteins associated with resistance (Tsr, Tar, CheA, and CheW), OmpF, and FliC of multidrug-resistant E. coli. At the same time, we proved that CPA 19 improves the sensitivity of E. coli to antibiotics (ceftriaxone sodium, amoxicillin, fosfomycin, sulfamonomethoxine, gatifloxacin, lincomycin, florfenicol, cefotaxime sodium, and rifampicin), causes the flagellum to fall off, breaks the structure of the cell wall and cell membrane, and leads to macromolecules leaks from the cell. This evidence elaborated the potential therapeutic efficacy of CPA 19 and provided a significant contribution to the discovery of antibacterial agents.Geyin ZhangYunqiao YangFareed Uddin MemonKaiyuan HaoBaichang XuShuaiyang WangYing WangEnyun WuXiaogang ChenWenguang XiongHongbin SiFrontiers Media S.A.articlecompound phenolic acidsEscherichia coliTMTantibacterial mechanismnew antimicrobial agentMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	compound phenolic acids Escherichia coli TMT antibacterial mechanism new antimicrobial agent Microbiology QR1-502
spellingShingle	compound phenolic acids Escherichia coli TMT antibacterial mechanism new antimicrobial agent Microbiology QR1-502 Geyin Zhang Yunqiao Yang Fareed Uddin Memon Kaiyuan Hao Baichang Xu Shuaiyang Wang Ying Wang Enyun Wu Xiaogang Chen Wenguang Xiong Hongbin Si A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics
description	The objective of this study was to evaluate the antibacterial mechanisms of phenolic acids as natural approaches against multi-drug resistant Escherichia coli (E. coli). For that purpose, five phenolic acids were combined with each other and 31 combinations were obtained in total. To select the most potent and effective combination, all of the obtained combinations were examined for minimum inhibitory concentration (MIC) and it was found that the compound phenolic acid (CPA) 19 (protocatechuic acid, hydrocinnamic acid, and chlorogenic acid at concentrations of 0.833, 0.208, and 1.677 mg/mL, respectively) showed better efficacy against E. coli compared to other combinations. Furthermore, based on tandem mass tag (TMT) proteomics, the treatment of CPA 19 significantly downregulated the proteins associated with resistance (Tsr, Tar, CheA, and CheW), OmpF, and FliC of multidrug-resistant E. coli. At the same time, we proved that CPA 19 improves the sensitivity of E. coli to antibiotics (ceftriaxone sodium, amoxicillin, fosfomycin, sulfamonomethoxine, gatifloxacin, lincomycin, florfenicol, cefotaxime sodium, and rifampicin), causes the flagellum to fall off, breaks the structure of the cell wall and cell membrane, and leads to macromolecules leaks from the cell. This evidence elaborated the potential therapeutic efficacy of CPA 19 and provided a significant contribution to the discovery of antibacterial agents.
format	article
author	Geyin Zhang Yunqiao Yang Fareed Uddin Memon Kaiyuan Hao Baichang Xu Shuaiyang Wang Ying Wang Enyun Wu Xiaogang Chen Wenguang Xiong Hongbin Si
author_facet	Geyin Zhang Yunqiao Yang Fareed Uddin Memon Kaiyuan Hao Baichang Xu Shuaiyang Wang Ying Wang Enyun Wu Xiaogang Chen Wenguang Xiong Hongbin Si
author_sort	Geyin Zhang
title	A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics
title_short	A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics
title_full	A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics
title_fullStr	A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics
title_full_unstemmed	A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics
title_sort	natural antimicrobial agent: analysis of antibacterial effect and mechanism of compound phenolic acid on escherichia coli based on tandem mass tag proteomics
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/732e23e0f1d747b8b5f213f7fc70e661
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A Natural Antimicrobial Agent: Analysis of Antibacterial Effect and Mechanism of Compound Phenolic Acid on Escherichia coli Based on Tandem Mass Tag Proteomics

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