An integrated multi-omics study revealed metabolic alterations underlying the effects of coffee consumption.

Many epidemiological studies have indicated that coffee consumption may reduce the risks of developing obesity and diabetes, but the underlying mechanisms of these effects are poorly understood. Our previous study revealed the changes on gene expression profiles in the livers of C57BL/6J mice fed a...

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Autores principales:	Shoko Takahashi, Kenji Saito, Huijuan Jia, Hisanori Kato
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/732f5a48482648048df33584d530993c
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spelling	oai:doaj.org-article:732f5a48482648048df33584d530993c2021-11-18T08:28:37ZAn integrated multi-omics study revealed metabolic alterations underlying the effects of coffee consumption.1932-620310.1371/journal.pone.0091134https://doaj.org/article/732f5a48482648048df33584d530993c2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24618914/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Many epidemiological studies have indicated that coffee consumption may reduce the risks of developing obesity and diabetes, but the underlying mechanisms of these effects are poorly understood. Our previous study revealed the changes on gene expression profiles in the livers of C57BL/6J mice fed a high-fat diet containing three types of coffee (caffeinated, decaffeinated and green unroasted coffee), using DNA microarrays. The results revealed remarkable alterations in lipid metabolism-related molecules which may be involved in the anti-obesity effects of coffee. We conducted the present study to further elucidate the metabolic alterations underlying the effects of coffee consumption through comprehensive proteomic and metabolomic analyses. Proteomics revealed an up-regulation of isocitrate dehydrogenase (a key enzyme in the TCA cycle) and its related proteins, suggesting increased energy generation. The metabolomics showed an up-regulation of metabolites involved in the urea cycle, with which the transcriptome data were highly consistent, indicating accelerated energy expenditure. The TCA cycle and the urea cycle are likely be accelerated in a concerted manner, since they are directly connected by mutually providing each other's intermediates. The up-regulation of these pathways might result in a metabolic shift causing increased ATP turnover, which is related to the alterations of lipid metabolism. This mechanism may play an important part in the suppressive effects of coffee consumption on obesity, inflammation, and hepatosteatosis. This study newly revealed global metabolic alterations induced by coffee intake, providing significant insights into the association between coffee intake and the prevention of type 2 diabetes, utilizing the benefits of multi-omics analyses.Shoko TakahashiKenji SaitoHuijuan JiaHisanori KatoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91134 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shoko Takahashi Kenji Saito Huijuan Jia Hisanori Kato An integrated multi-omics study revealed metabolic alterations underlying the effects of coffee consumption.
description	Many epidemiological studies have indicated that coffee consumption may reduce the risks of developing obesity and diabetes, but the underlying mechanisms of these effects are poorly understood. Our previous study revealed the changes on gene expression profiles in the livers of C57BL/6J mice fed a high-fat diet containing three types of coffee (caffeinated, decaffeinated and green unroasted coffee), using DNA microarrays. The results revealed remarkable alterations in lipid metabolism-related molecules which may be involved in the anti-obesity effects of coffee. We conducted the present study to further elucidate the metabolic alterations underlying the effects of coffee consumption through comprehensive proteomic and metabolomic analyses. Proteomics revealed an up-regulation of isocitrate dehydrogenase (a key enzyme in the TCA cycle) and its related proteins, suggesting increased energy generation. The metabolomics showed an up-regulation of metabolites involved in the urea cycle, with which the transcriptome data were highly consistent, indicating accelerated energy expenditure. The TCA cycle and the urea cycle are likely be accelerated in a concerted manner, since they are directly connected by mutually providing each other's intermediates. The up-regulation of these pathways might result in a metabolic shift causing increased ATP turnover, which is related to the alterations of lipid metabolism. This mechanism may play an important part in the suppressive effects of coffee consumption on obesity, inflammation, and hepatosteatosis. This study newly revealed global metabolic alterations induced by coffee intake, providing significant insights into the association between coffee intake and the prevention of type 2 diabetes, utilizing the benefits of multi-omics analyses.
format	article
author	Shoko Takahashi Kenji Saito Huijuan Jia Hisanori Kato
author_facet	Shoko Takahashi Kenji Saito Huijuan Jia Hisanori Kato
author_sort	Shoko Takahashi
title	An integrated multi-omics study revealed metabolic alterations underlying the effects of coffee consumption.
title_short	An integrated multi-omics study revealed metabolic alterations underlying the effects of coffee consumption.
title_full	An integrated multi-omics study revealed metabolic alterations underlying the effects of coffee consumption.
title_fullStr	An integrated multi-omics study revealed metabolic alterations underlying the effects of coffee consumption.
title_full_unstemmed	An integrated multi-omics study revealed metabolic alterations underlying the effects of coffee consumption.
title_sort	integrated multi-omics study revealed metabolic alterations underlying the effects of coffee consumption.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/732f5a48482648048df33584d530993c
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An integrated multi-omics study revealed metabolic alterations underlying the effects of coffee consumption.

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