Characterization of BSL6 isolates isolated from honeybee hive and to determine its antibacterial activity

Abstract. Fitri L, Yasmin Y, Fauziah, Septiani DA, Suhartono. 2020. Characterization of BSL6 isolates isolated from honeybee hive and to determine its antibacterial activity. Biodiversitas 21: 4859-4865. This study was aimed to characterize BSL6 isolate of bacteria isolated from honeybee hive, and t...

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Autores principales:	Lenni Fitri, YEKKI YASMIN, FAUZIAH FAUZIAH, DWI ANDRI SEPTIANI, SUHARTONO SUHARTONO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	bsl6, characterization, honeybee hive, minimum inhibitory concentration (mic), secondary metabolites Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/73341d9c0ade4b11b68df4db074af2cc
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spelling	oai:doaj.org-article:73341d9c0ade4b11b68df4db074af2cc2021-11-22T00:51:08ZCharacterization of BSL6 isolates isolated from honeybee hive and to determine its antibacterial activity1412-033X2085-472210.13057/biodiv/d211052https://doaj.org/article/73341d9c0ade4b11b68df4db074af2cc2020-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6775https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Fitri L, Yasmin Y, Fauziah, Septiani DA, Suhartono. 2020. Characterization of BSL6 isolates isolated from honeybee hive and to determine its antibacterial activity. Biodiversitas 21: 4859-4865. This study was aimed to characterize BSL6 isolate of bacteria isolated from honeybee hive, and to determine its antimicrobial activity against Staphylococcus aureus and Escherichia coli. In addition, the Minimum Inhibitory Concentration (MIC) value of BSL6 isolate was determined against S. aureus, and content of secondary metabolic compounds was also determined. Characterization was conducted in this study on the basis of morphological observations, physiological tests, and 16S rDNA. The method used in determining MIC value was completely randomized design (CRD) then analyzed by ANOVA test using SPSS application and followed by Tukey test. The analysis conducted to determine the content of secondary metabolites was descriptive type. The results showed that BSL6 isolate morphologically and physiologically belonged to the genus Bacillus, and 16S rDNA test results showed that the isolate had the highest similarity with Bacillus siamensis strain 64X-5. BSL6 isolate was able to inhibit the growth of S. aureus with inhibition zone diameter of 17.87 mm. MIC value of BSL6 against S. aureus was at a concentration of 12.5%. The content of secondary metabolite compounds from BSL6 extract was saponins.Lenni FitriYEKKI YASMINFAUZIAH FAUZIAHDWI ANDRI SEPTIANISUHARTONO SUHARTONOMBI & UNS Soloarticlebsl6, characterization, honeybee hive, minimum inhibitory concentration (mic), secondary metabolitesBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bsl6, characterization, honeybee hive, minimum inhibitory concentration (mic), secondary metabolites Biology (General) QH301-705.5
spellingShingle	bsl6, characterization, honeybee hive, minimum inhibitory concentration (mic), secondary metabolites Biology (General) QH301-705.5 Lenni Fitri YEKKI YASMIN FAUZIAH FAUZIAH DWI ANDRI SEPTIANI SUHARTONO SUHARTONO Characterization of BSL6 isolates isolated from honeybee hive and to determine its antibacterial activity
description	Abstract. Fitri L, Yasmin Y, Fauziah, Septiani DA, Suhartono. 2020. Characterization of BSL6 isolates isolated from honeybee hive and to determine its antibacterial activity. Biodiversitas 21: 4859-4865. This study was aimed to characterize BSL6 isolate of bacteria isolated from honeybee hive, and to determine its antimicrobial activity against Staphylococcus aureus and Escherichia coli. In addition, the Minimum Inhibitory Concentration (MIC) value of BSL6 isolate was determined against S. aureus, and content of secondary metabolic compounds was also determined. Characterization was conducted in this study on the basis of morphological observations, physiological tests, and 16S rDNA. The method used in determining MIC value was completely randomized design (CRD) then analyzed by ANOVA test using SPSS application and followed by Tukey test. The analysis conducted to determine the content of secondary metabolites was descriptive type. The results showed that BSL6 isolate morphologically and physiologically belonged to the genus Bacillus, and 16S rDNA test results showed that the isolate had the highest similarity with Bacillus siamensis strain 64X-5. BSL6 isolate was able to inhibit the growth of S. aureus with inhibition zone diameter of 17.87 mm. MIC value of BSL6 against S. aureus was at a concentration of 12.5%. The content of secondary metabolite compounds from BSL6 extract was saponins.
format	article
author	Lenni Fitri YEKKI YASMIN FAUZIAH FAUZIAH DWI ANDRI SEPTIANI SUHARTONO SUHARTONO
author_facet	Lenni Fitri YEKKI YASMIN FAUZIAH FAUZIAH DWI ANDRI SEPTIANI SUHARTONO SUHARTONO
author_sort	Lenni Fitri
title	Characterization of BSL6 isolates isolated from honeybee hive and to determine its antibacterial activity
title_short	Characterization of BSL6 isolates isolated from honeybee hive and to determine its antibacterial activity
title_full	Characterization of BSL6 isolates isolated from honeybee hive and to determine its antibacterial activity
title_fullStr	Characterization of BSL6 isolates isolated from honeybee hive and to determine its antibacterial activity
title_full_unstemmed	Characterization of BSL6 isolates isolated from honeybee hive and to determine its antibacterial activity
title_sort	characterization of bsl6 isolates isolated from honeybee hive and to determine its antibacterial activity
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/73341d9c0ade4b11b68df4db074af2cc
work_keys_str_mv	AT lennifitri characterizationofbsl6isolatesisolatedfromhoneybeehiveandtodetermineitsantibacterialactivity AT yekkiyasmin characterizationofbsl6isolatesisolatedfromhoneybeehiveandtodetermineitsantibacterialactivity AT fauziahfauziah characterizationofbsl6isolatesisolatedfromhoneybeehiveandtodetermineitsantibacterialactivity AT dwiandriseptiani characterizationofbsl6isolatesisolatedfromhoneybeehiveandtodetermineitsantibacterialactivity AT suhartonosuhartono characterizationofbsl6isolatesisolatedfromhoneybeehiveandtodetermineitsantibacterialactivity
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Characterization of BSL6 isolates isolated from honeybee hive and to determine its antibacterial activity

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