A YoeB toxin cleaves both RNA and DNA

Abstract Type II toxin-antitoxin systems contain a toxin protein, which mediates diverse interactions within the bacterial cell when it is not bound by its cognate antitoxin protein. These toxins provide a rich source of evolutionarily-conserved tertiary folds that mediate diverse catalytic reaction...

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Autores principales:	Julia McGillick, Jessica R. Ames, Tamiko Murphy, Christina R. Bourne
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/733b37a7942248448dfa03ba46406016
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spelling	oai:doaj.org-article:733b37a7942248448dfa03ba464060162021-12-02T12:09:32ZA YoeB toxin cleaves both RNA and DNA10.1038/s41598-021-82950-62045-2322https://doaj.org/article/733b37a7942248448dfa03ba464060162021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82950-6https://doaj.org/toc/2045-2322Abstract Type II toxin-antitoxin systems contain a toxin protein, which mediates diverse interactions within the bacterial cell when it is not bound by its cognate antitoxin protein. These toxins provide a rich source of evolutionarily-conserved tertiary folds that mediate diverse catalytic reactions. These properties make toxins of interest in biotechnology applications, and studies of the catalytic mechanisms continue to provide surprises. In the current work, our studies on a YoeB family toxin from Agrobacterium tumefaciens have revealed a conserved ribosome-independent non-specific nuclease activity. We have quantified the RNA and DNA cleavage activity, revealing they have essentially equivalent dose-dependence while differing in requirements for divalent cations and pH sensitivity. The DNA cleavage activity is as a nickase for any topology of double-stranded DNA, as well as cleaving single-stranded DNA. AtYoeB is able to bind to double-stranded DNA with mid-micromolar affinity. Comparison of the ribosome-dependent and -independent reactions demonstrates an approximate tenfold efficiency imparted by the ribosome. This demonstrates YoeB toxins can act as non-specific nucleases, cleaving both RNA and DNA, in the absence of being bound within the ribosome.Julia McGillickJessica R. AmesTamiko MurphyChristina R. BourneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julia McGillick Jessica R. Ames Tamiko Murphy Christina R. Bourne A YoeB toxin cleaves both RNA and DNA
description	Abstract Type II toxin-antitoxin systems contain a toxin protein, which mediates diverse interactions within the bacterial cell when it is not bound by its cognate antitoxin protein. These toxins provide a rich source of evolutionarily-conserved tertiary folds that mediate diverse catalytic reactions. These properties make toxins of interest in biotechnology applications, and studies of the catalytic mechanisms continue to provide surprises. In the current work, our studies on a YoeB family toxin from Agrobacterium tumefaciens have revealed a conserved ribosome-independent non-specific nuclease activity. We have quantified the RNA and DNA cleavage activity, revealing they have essentially equivalent dose-dependence while differing in requirements for divalent cations and pH sensitivity. The DNA cleavage activity is as a nickase for any topology of double-stranded DNA, as well as cleaving single-stranded DNA. AtYoeB is able to bind to double-stranded DNA with mid-micromolar affinity. Comparison of the ribosome-dependent and -independent reactions demonstrates an approximate tenfold efficiency imparted by the ribosome. This demonstrates YoeB toxins can act as non-specific nucleases, cleaving both RNA and DNA, in the absence of being bound within the ribosome.
format	article
author	Julia McGillick Jessica R. Ames Tamiko Murphy Christina R. Bourne
author_facet	Julia McGillick Jessica R. Ames Tamiko Murphy Christina R. Bourne
author_sort	Julia McGillick
title	A YoeB toxin cleaves both RNA and DNA
title_short	A YoeB toxin cleaves both RNA and DNA
title_full	A YoeB toxin cleaves both RNA and DNA
title_fullStr	A YoeB toxin cleaves both RNA and DNA
title_full_unstemmed	A YoeB toxin cleaves both RNA and DNA
title_sort	yoeb toxin cleaves both rna and dna
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/733b37a7942248448dfa03ba46406016
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A YoeB toxin cleaves both RNA and DNA

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