Ndrg1 promotes adipocyte differentiation and sustains their function

Abstract Adipocytes play a central role in maintaining metabolic homeostasis in the body. Differentiation of adipocyte precursor cells requires the transcriptional activity of peroxisome proliferator-activated receptor-γ (Pparγ) and CCAAT/enhancer binding proteins (C/Ebps). Transcriptional activity...

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Autores principales:	Kai Cai, Rabih El-Merahbi, Mona Loeffler, Alexander E. Mayer, Grzegorz Sumara
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7340487d663a48fda64cf5ea5b1af6af
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spelling	oai:doaj.org-article:7340487d663a48fda64cf5ea5b1af6af2021-12-02T16:06:49ZNdrg1 promotes adipocyte differentiation and sustains their function10.1038/s41598-017-07497-x2045-2322https://doaj.org/article/7340487d663a48fda64cf5ea5b1af6af2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07497-xhttps://doaj.org/toc/2045-2322Abstract Adipocytes play a central role in maintaining metabolic homeostasis in the body. Differentiation of adipocyte precursor cells requires the transcriptional activity of peroxisome proliferator-activated receptor-γ (Pparγ) and CCAAT/enhancer binding proteins (C/Ebps). Transcriptional activity is regulated by signaling modules activated by a plethora of hormones and nutrients. Mechanistic target of rapamacin complexes (mTORC) 1 and 2 are central for the coordination of hormonal and nutritional inputs in cells and are essential for adipogenesis. Serum glucocorticoid kinase 1 (Sgk1)-dependent phosphorylation of N-Myc downstream-regulated gene 1 (Ndrg1) is a hallmark of mTORC2 activation in cells. Moreover, Pparγ activation promotes Ndrg1 expression. However, the impact of Ndrg1 on adipocyte differentiation and function has not yet been defined. Here, we show that Ndrg1 expression and its Sgk1-dependent phosphorylation are induced during adipogenesis. Consistently, we demonstrate that Ndrg1 promotes adipocyte differentiation and function by inducing Pparγ expression. Additionally, our results indicate that Ndrg1 is required for C/Ebpα phosphorylation. Moreover, we found that Ndrg1 phosphorylation by Sgk1 promotes adipocyte formation. Taken together, we show that induction of Ndrg1 expression by Pparγ and its phosphorylation by Sgk1 kinase are required for the acquisition of adipocyte characteristics by precursor cells.Kai CaiRabih El-MerahbiMona LoefflerAlexander E. MayerGrzegorz SumaraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kai Cai Rabih El-Merahbi Mona Loeffler Alexander E. Mayer Grzegorz Sumara Ndrg1 promotes adipocyte differentiation and sustains their function
description	Abstract Adipocytes play a central role in maintaining metabolic homeostasis in the body. Differentiation of adipocyte precursor cells requires the transcriptional activity of peroxisome proliferator-activated receptor-γ (Pparγ) and CCAAT/enhancer binding proteins (C/Ebps). Transcriptional activity is regulated by signaling modules activated by a plethora of hormones and nutrients. Mechanistic target of rapamacin complexes (mTORC) 1 and 2 are central for the coordination of hormonal and nutritional inputs in cells and are essential for adipogenesis. Serum glucocorticoid kinase 1 (Sgk1)-dependent phosphorylation of N-Myc downstream-regulated gene 1 (Ndrg1) is a hallmark of mTORC2 activation in cells. Moreover, Pparγ activation promotes Ndrg1 expression. However, the impact of Ndrg1 on adipocyte differentiation and function has not yet been defined. Here, we show that Ndrg1 expression and its Sgk1-dependent phosphorylation are induced during adipogenesis. Consistently, we demonstrate that Ndrg1 promotes adipocyte differentiation and function by inducing Pparγ expression. Additionally, our results indicate that Ndrg1 is required for C/Ebpα phosphorylation. Moreover, we found that Ndrg1 phosphorylation by Sgk1 promotes adipocyte formation. Taken together, we show that induction of Ndrg1 expression by Pparγ and its phosphorylation by Sgk1 kinase are required for the acquisition of adipocyte characteristics by precursor cells.
format	article
author	Kai Cai Rabih El-Merahbi Mona Loeffler Alexander E. Mayer Grzegorz Sumara
author_facet	Kai Cai Rabih El-Merahbi Mona Loeffler Alexander E. Mayer Grzegorz Sumara
author_sort	Kai Cai
title	Ndrg1 promotes adipocyte differentiation and sustains their function
title_short	Ndrg1 promotes adipocyte differentiation and sustains their function
title_full	Ndrg1 promotes adipocyte differentiation and sustains their function
title_fullStr	Ndrg1 promotes adipocyte differentiation and sustains their function
title_full_unstemmed	Ndrg1 promotes adipocyte differentiation and sustains their function
title_sort	ndrg1 promotes adipocyte differentiation and sustains their function
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7340487d663a48fda64cf5ea5b1af6af
work_keys_str_mv	AT kaicai ndrg1promotesadipocytedifferentiationandsustainstheirfunction AT rabihelmerahbi ndrg1promotesadipocytedifferentiationandsustainstheirfunction AT monaloeffler ndrg1promotesadipocytedifferentiationandsustainstheirfunction AT alexanderemayer ndrg1promotesadipocytedifferentiationandsustainstheirfunction AT grzegorzsumara ndrg1promotesadipocytedifferentiationandsustainstheirfunction
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Ndrg1 promotes adipocyte differentiation and sustains their function

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