Broad expression analysis of human ANTXR1/TEM8 transcripts reveals differential expression and novel splizce variants.

Tumor endothelial marker 8 (TEM8; ANTXR1) is one of two anthrax toxin receptors; the other is capillary morphogenesis gene 2 protein (CMG2; ANTXR2). TEM8 shows enhanced expression in certain tumor endothelia, and is thought to be a player in tumor vasculature formation. However, a comprehensive expr...

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Autores principales:	Micaela Vargas, Raghavendra Karamsetty, Stephen H Leppla, G Jilani Chaudry
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/73468f32698a46e98d6ab966b1fa6f9b
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spelling	oai:doaj.org-article:73468f32698a46e98d6ab966b1fa6f9b2021-11-18T07:08:26ZBroad expression analysis of human ANTXR1/TEM8 transcripts reveals differential expression and novel splizce variants.1932-620310.1371/journal.pone.0043174https://doaj.org/article/73468f32698a46e98d6ab966b1fa6f9b2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22912819/?tool=EBIhttps://doaj.org/toc/1932-6203Tumor endothelial marker 8 (TEM8; ANTXR1) is one of two anthrax toxin receptors; the other is capillary morphogenesis gene 2 protein (CMG2; ANTXR2). TEM8 shows enhanced expression in certain tumor endothelia, and is thought to be a player in tumor vasculature formation. However, a comprehensive expression profile of individual TEM8 variants in normal or cancerous tissues is lacking. In this work we carried out an extensive analysis of all splice variants of human TEM8 in 12 digestive tissues, and 8 each fetal and adult tissues, 6 of them cognate pairs. Using variant-specific primers, we first ascertained the status of full-length transcripts by nested PCR. We then carried out quantitative analysis of each transcript by real-time PCR. Three splice variants of TEM8 were reported before, two single-pass integral membrane forms (V1 and V2) and one secreted (V3). Our analysis has revealed two new variants, one encoding a membrane-bound form of the receptor and the other secreted, which we have designated V4 and V5, respectively. All tissues had V1, V2, V3, and V4, but only prostate had V5. Real-time PCR revealed that all variants are present at different levels in various tissues. V3 appeared the most abundant of all. To ascertain its functionality for anthrax toxin, we expressed the newly identified form V4 in a receptor-negative host cell, and included V1 and V2 for comparison. Cytotoxicity, toxin binding, and internalization assays showed V4 to be as efficient a receptor as V1 and V2.Micaela VargasRaghavendra KaramsettyStephen H LepplaG Jilani ChaudryPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e43174 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Micaela Vargas Raghavendra Karamsetty Stephen H Leppla G Jilani Chaudry Broad expression analysis of human ANTXR1/TEM8 transcripts reveals differential expression and novel splizce variants.
description	Tumor endothelial marker 8 (TEM8; ANTXR1) is one of two anthrax toxin receptors; the other is capillary morphogenesis gene 2 protein (CMG2; ANTXR2). TEM8 shows enhanced expression in certain tumor endothelia, and is thought to be a player in tumor vasculature formation. However, a comprehensive expression profile of individual TEM8 variants in normal or cancerous tissues is lacking. In this work we carried out an extensive analysis of all splice variants of human TEM8 in 12 digestive tissues, and 8 each fetal and adult tissues, 6 of them cognate pairs. Using variant-specific primers, we first ascertained the status of full-length transcripts by nested PCR. We then carried out quantitative analysis of each transcript by real-time PCR. Three splice variants of TEM8 were reported before, two single-pass integral membrane forms (V1 and V2) and one secreted (V3). Our analysis has revealed two new variants, one encoding a membrane-bound form of the receptor and the other secreted, which we have designated V4 and V5, respectively. All tissues had V1, V2, V3, and V4, but only prostate had V5. Real-time PCR revealed that all variants are present at different levels in various tissues. V3 appeared the most abundant of all. To ascertain its functionality for anthrax toxin, we expressed the newly identified form V4 in a receptor-negative host cell, and included V1 and V2 for comparison. Cytotoxicity, toxin binding, and internalization assays showed V4 to be as efficient a receptor as V1 and V2.
format	article
author	Micaela Vargas Raghavendra Karamsetty Stephen H Leppla G Jilani Chaudry
author_facet	Micaela Vargas Raghavendra Karamsetty Stephen H Leppla G Jilani Chaudry
author_sort	Micaela Vargas
title	Broad expression analysis of human ANTXR1/TEM8 transcripts reveals differential expression and novel splizce variants.
title_short	Broad expression analysis of human ANTXR1/TEM8 transcripts reveals differential expression and novel splizce variants.
title_full	Broad expression analysis of human ANTXR1/TEM8 transcripts reveals differential expression and novel splizce variants.
title_fullStr	Broad expression analysis of human ANTXR1/TEM8 transcripts reveals differential expression and novel splizce variants.
title_full_unstemmed	Broad expression analysis of human ANTXR1/TEM8 transcripts reveals differential expression and novel splizce variants.
title_sort	broad expression analysis of human antxr1/tem8 transcripts reveals differential expression and novel splizce variants.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/73468f32698a46e98d6ab966b1fa6f9b
work_keys_str_mv	AT micaelavargas broadexpressionanalysisofhumanantxr1tem8transcriptsrevealsdifferentialexpressionandnovelsplizcevariants AT raghavendrakaramsetty broadexpressionanalysisofhumanantxr1tem8transcriptsrevealsdifferentialexpressionandnovelsplizcevariants AT stephenhleppla broadexpressionanalysisofhumanantxr1tem8transcriptsrevealsdifferentialexpressionandnovelsplizcevariants AT gjilanichaudry broadexpressionanalysisofhumanantxr1tem8transcriptsrevealsdifferentialexpressionandnovelsplizcevariants
_version_	1718423857589649408

Broad expression analysis of human ANTXR1/TEM8 transcripts reveals differential expression and novel splizce variants.

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