Electrochemical Hydroxylation of C3–C12 n-Alkanes by Recombinant Alkane Hydroxylase (AlkB) and Rubredoxin-2 (AlkG) from Pseudomonas putida GPo1

Abstract An unprecedented method for the efficient conversion of C3–C12 linear alkanes to their corresponding primary alcohols mediated by the membrane-bound alkane hydroxylase (AlkB) from Pseudomonas putida GPo1 is demonstrated. The X-ray absorption spectroscopy (XAS) studies support that electrons...

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Autores principales:	Yi-Fang Tsai, Wen-I Luo, Jen-Lin Chang, Chun-Wei Chang, Huai-Chun Chuang, Ravirala Ramu, Guor-Tzo Wei, Jyh-Myng Zen, Steve S.-F. Yu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7349630be981486ab517091a9f28b575
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spelling	oai:doaj.org-article:7349630be981486ab517091a9f28b5752021-12-02T12:32:24ZElectrochemical Hydroxylation of C3–C12 n-Alkanes by Recombinant Alkane Hydroxylase (AlkB) and Rubredoxin-2 (AlkG) from Pseudomonas putida GPo110.1038/s41598-017-08610-w2045-2322https://doaj.org/article/7349630be981486ab517091a9f28b5752017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08610-whttps://doaj.org/toc/2045-2322Abstract An unprecedented method for the efficient conversion of C3–C12 linear alkanes to their corresponding primary alcohols mediated by the membrane-bound alkane hydroxylase (AlkB) from Pseudomonas putida GPo1 is demonstrated. The X-ray absorption spectroscopy (XAS) studies support that electrons can be transferred from the reduced AlkG (rubredoxin-2, the redox partner of AlkB) to AlkB in a two-phase manner. Based on this observation, an approach for the electrocatalytic conversion from alkanes to alcohols mediated by AlkB using an AlkG immobilized screen-printed carbon electrode (SPCE) is developed. The framework distortion of AlkB–AlkG adduct on SPCE surface might create promiscuity toward gaseous substrates. Hence, small alkanes including propane and n-butane can be accommodated in the hydrophobic pocket of AlkB for C–H bond activation. The proof of concept herein advances the development of artificial C–H bond activation catalysts.Yi-Fang TsaiWen-I LuoJen-Lin ChangChun-Wei ChangHuai-Chun ChuangRavirala RamuGuor-Tzo WeiJyh-Myng ZenSteve S.-F. YuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi-Fang Tsai Wen-I Luo Jen-Lin Chang Chun-Wei Chang Huai-Chun Chuang Ravirala Ramu Guor-Tzo Wei Jyh-Myng Zen Steve S.-F. Yu Electrochemical Hydroxylation of C3–C12 n-Alkanes by Recombinant Alkane Hydroxylase (AlkB) and Rubredoxin-2 (AlkG) from Pseudomonas putida GPo1
description	Abstract An unprecedented method for the efficient conversion of C3–C12 linear alkanes to their corresponding primary alcohols mediated by the membrane-bound alkane hydroxylase (AlkB) from Pseudomonas putida GPo1 is demonstrated. The X-ray absorption spectroscopy (XAS) studies support that electrons can be transferred from the reduced AlkG (rubredoxin-2, the redox partner of AlkB) to AlkB in a two-phase manner. Based on this observation, an approach for the electrocatalytic conversion from alkanes to alcohols mediated by AlkB using an AlkG immobilized screen-printed carbon electrode (SPCE) is developed. The framework distortion of AlkB–AlkG adduct on SPCE surface might create promiscuity toward gaseous substrates. Hence, small alkanes including propane and n-butane can be accommodated in the hydrophobic pocket of AlkB for C–H bond activation. The proof of concept herein advances the development of artificial C–H bond activation catalysts.
format	article
author	Yi-Fang Tsai Wen-I Luo Jen-Lin Chang Chun-Wei Chang Huai-Chun Chuang Ravirala Ramu Guor-Tzo Wei Jyh-Myng Zen Steve S.-F. Yu
author_facet	Yi-Fang Tsai Wen-I Luo Jen-Lin Chang Chun-Wei Chang Huai-Chun Chuang Ravirala Ramu Guor-Tzo Wei Jyh-Myng Zen Steve S.-F. Yu
author_sort	Yi-Fang Tsai
title	Electrochemical Hydroxylation of C3–C12 n-Alkanes by Recombinant Alkane Hydroxylase (AlkB) and Rubredoxin-2 (AlkG) from Pseudomonas putida GPo1
title_short	Electrochemical Hydroxylation of C3–C12 n-Alkanes by Recombinant Alkane Hydroxylase (AlkB) and Rubredoxin-2 (AlkG) from Pseudomonas putida GPo1
title_full	Electrochemical Hydroxylation of C3–C12 n-Alkanes by Recombinant Alkane Hydroxylase (AlkB) and Rubredoxin-2 (AlkG) from Pseudomonas putida GPo1
title_fullStr	Electrochemical Hydroxylation of C3–C12 n-Alkanes by Recombinant Alkane Hydroxylase (AlkB) and Rubredoxin-2 (AlkG) from Pseudomonas putida GPo1
title_full_unstemmed	Electrochemical Hydroxylation of C3–C12 n-Alkanes by Recombinant Alkane Hydroxylase (AlkB) and Rubredoxin-2 (AlkG) from Pseudomonas putida GPo1
title_sort	electrochemical hydroxylation of c3–c12 n-alkanes by recombinant alkane hydroxylase (alkb) and rubredoxin-2 (alkg) from pseudomonas putida gpo1
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7349630be981486ab517091a9f28b575
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Electrochemical Hydroxylation of C3–C12 n-Alkanes by Recombinant Alkane Hydroxylase (AlkB) and Rubredoxin-2 (AlkG) from Pseudomonas putida GPo1

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