Zika virus exposure affects neuron-glia communication in the hippocampal slices of adult rats

Abstract Zika virus (ZIKV) infection during pregnancy was associated with microcephaly in neonates, but clinical and experimental evidence indicate that ZIKV also causes neurological complications in adults. However, the changes in neuron-glial communication, which is essential for brain homeostasis...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Larissa Daniele Bobermin, André Quincozes-Santos, Camila Leite Santos, Ana Paula M. Varela, Thais F. Teixeira, Krista Minéia Wartchow, Lílian Juliana Lissner, Amanda da Silva, Natalie K. Thomaz, Lucélia Santi, Walter O. Beys-da-Silva, Paulo M. Roehe, Patrícia Sesterheim, Jorge A. Guimarães, Carlos-Alberto Gonçalves, Diogo Onofre Souza
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7355227ebb4649638543ccf12321e6ad
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7355227ebb4649638543ccf12321e6ad
record_format	dspace
spelling	oai:doaj.org-article:7355227ebb4649638543ccf12321e6ad2021-12-02T11:43:44ZZika virus exposure affects neuron-glia communication in the hippocampal slices of adult rats10.1038/s41598-020-78735-y2045-2322https://doaj.org/article/7355227ebb4649638543ccf12321e6ad2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78735-yhttps://doaj.org/toc/2045-2322Abstract Zika virus (ZIKV) infection during pregnancy was associated with microcephaly in neonates, but clinical and experimental evidence indicate that ZIKV also causes neurological complications in adults. However, the changes in neuron-glial communication, which is essential for brain homeostasis, are still unknown. Here, we report that hippocampal slices from adult rats exposed acutely to ZIKV showed significant cellular alterations regarding to redox homeostasis, inflammatory process, neurotrophic functions and molecular signalling pathways associated with neurons and glial cells. Our findings support the hypothesis that ZIKV is highly neurotropic and its infection readily induces an inflammatory response, characterized by an increased expression and/or release of pro-inflammatory cytokines. We also observed changes in neural parameters, such as adenosine receptor A2a expression, as well as in the release of brain-derived neurotrophic factor and neuron-specific enolase, indicating plasticity synaptic impairment/neuronal damage. In addition, ZIKV induced a glial commitment, with alterations in specific and functional parameters such as aquaporin 4 expression, S100B secretion and glutathione synthesis. ZIKV also induced p21 senescence-associated gene expression, indicating that ZIKV may induce early senescence. Taken together, our results indicate that ZIKV-induced neuroinflammation, involving nuclear factor erythroid 2-related factor 2 (Nrf2) and nuclear factor κB (NFκB) pathways, affects important aspects of neuron-glia communication. Therefore, although ZIKV infection is transient, long-term consequences might be associated with neurological and/or neurodegenerative diseases.Larissa Daniele BoberminAndré Quincozes-SantosCamila Leite SantosAna Paula M. VarelaThais F. TeixeiraKrista Minéia WartchowLílian Juliana LissnerAmanda da SilvaNatalie K. ThomazLucélia SantiWalter O. Beys-da-SilvaPaulo M. RoehePatrícia SesterheimJorge A. GuimarãesCarlos-Alberto GonçalvesDiogo Onofre SouzaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Larissa Daniele Bobermin André Quincozes-Santos Camila Leite Santos Ana Paula M. Varela Thais F. Teixeira Krista Minéia Wartchow Lílian Juliana Lissner Amanda da Silva Natalie K. Thomaz Lucélia Santi Walter O. Beys-da-Silva Paulo M. Roehe Patrícia Sesterheim Jorge A. Guimarães Carlos-Alberto Gonçalves Diogo Onofre Souza Zika virus exposure affects neuron-glia communication in the hippocampal slices of adult rats
description	Abstract Zika virus (ZIKV) infection during pregnancy was associated with microcephaly in neonates, but clinical and experimental evidence indicate that ZIKV also causes neurological complications in adults. However, the changes in neuron-glial communication, which is essential for brain homeostasis, are still unknown. Here, we report that hippocampal slices from adult rats exposed acutely to ZIKV showed significant cellular alterations regarding to redox homeostasis, inflammatory process, neurotrophic functions and molecular signalling pathways associated with neurons and glial cells. Our findings support the hypothesis that ZIKV is highly neurotropic and its infection readily induces an inflammatory response, characterized by an increased expression and/or release of pro-inflammatory cytokines. We also observed changes in neural parameters, such as adenosine receptor A2a expression, as well as in the release of brain-derived neurotrophic factor and neuron-specific enolase, indicating plasticity synaptic impairment/neuronal damage. In addition, ZIKV induced a glial commitment, with alterations in specific and functional parameters such as aquaporin 4 expression, S100B secretion and glutathione synthesis. ZIKV also induced p21 senescence-associated gene expression, indicating that ZIKV may induce early senescence. Taken together, our results indicate that ZIKV-induced neuroinflammation, involving nuclear factor erythroid 2-related factor 2 (Nrf2) and nuclear factor κB (NFκB) pathways, affects important aspects of neuron-glia communication. Therefore, although ZIKV infection is transient, long-term consequences might be associated with neurological and/or neurodegenerative diseases.
format	article
author	Larissa Daniele Bobermin André Quincozes-Santos Camila Leite Santos Ana Paula M. Varela Thais F. Teixeira Krista Minéia Wartchow Lílian Juliana Lissner Amanda da Silva Natalie K. Thomaz Lucélia Santi Walter O. Beys-da-Silva Paulo M. Roehe Patrícia Sesterheim Jorge A. Guimarães Carlos-Alberto Gonçalves Diogo Onofre Souza
author_facet	Larissa Daniele Bobermin André Quincozes-Santos Camila Leite Santos Ana Paula M. Varela Thais F. Teixeira Krista Minéia Wartchow Lílian Juliana Lissner Amanda da Silva Natalie K. Thomaz Lucélia Santi Walter O. Beys-da-Silva Paulo M. Roehe Patrícia Sesterheim Jorge A. Guimarães Carlos-Alberto Gonçalves Diogo Onofre Souza
author_sort	Larissa Daniele Bobermin
title	Zika virus exposure affects neuron-glia communication in the hippocampal slices of adult rats
title_short	Zika virus exposure affects neuron-glia communication in the hippocampal slices of adult rats
title_full	Zika virus exposure affects neuron-glia communication in the hippocampal slices of adult rats
title_fullStr	Zika virus exposure affects neuron-glia communication in the hippocampal slices of adult rats
title_full_unstemmed	Zika virus exposure affects neuron-glia communication in the hippocampal slices of adult rats
title_sort	zika virus exposure affects neuron-glia communication in the hippocampal slices of adult rats
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/7355227ebb4649638543ccf12321e6ad
work_keys_str_mv	AT larissadanielebobermin zikavirusexposureaffectsneurongliacommunicationinthehippocampalslicesofadultrats AT andrequincozessantos zikavirusexposureaffectsneurongliacommunicationinthehippocampalslicesofadultrats AT camilaleitesantos zikavirusexposureaffectsneurongliacommunicationinthehippocampalslicesofadultrats AT anapaulamvarela zikavirusexposureaffectsneurongliacommunicationinthehippocampalslicesofadultrats AT thaisfteixeira zikavirusexposureaffectsneurongliacommunicationinthehippocampalslicesofadultrats AT kristamineiawartchow zikavirusexposureaffectsneurongliacommunicationinthehippocampalslicesofadultrats AT lilianjulianalissner zikavirusexposureaffectsneurongliacommunicationinthehippocampalslicesofadultrats AT amandadasilva zikavirusexposureaffectsneurongliacommunicationinthehippocampalslicesofadultrats AT nataliekthomaz zikavirusexposureaffectsneurongliacommunicationinthehippocampalslicesofadultrats AT luceliasanti zikavirusexposureaffectsneurongliacommunicationinthehippocampalslicesofadultrats AT walterobeysdasilva zikavirusexposureaffectsneurongliacommunicationinthehippocampalslicesofadultrats AT paulomroehe zikavirusexposureaffectsneurongliacommunicationinthehippocampalslicesofadultrats AT patriciasesterheim zikavirusexposureaffectsneurongliacommunicationinthehippocampalslicesofadultrats AT jorgeaguimaraes zikavirusexposureaffectsneurongliacommunicationinthehippocampalslicesofadultrats AT carlosalbertogoncalves zikavirusexposureaffectsneurongliacommunicationinthehippocampalslicesofadultrats AT diogoonofresouza zikavirusexposureaffectsneurongliacommunicationinthehippocampalslicesofadultrats
_version_	1718395348471250944

Zika virus exposure affects neuron-glia communication in the hippocampal slices of adult rats

Ejemplares similares