Composite polyelectrolyte multilayers for biofunctionalization of medical devices

Polyelectrolyte multilayer coatings (PEM) are prepared by alternative layer-by-layer deposition of cationic and anionic polyelectrolyte monolayers on charged surfaces. The thickness of the coatings ranges from nm to few μm. Their properties such as roughness, stiffness, surface charge and surface en...

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Autores principales:	Rudt Alexander, Andreeva Tonya D., Krastev Rumen, Taneva Stefka G.
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2020
Materias:	polyelectrolyte multilayers biocompatibility cell adhesion composite films graphene oxide Medicine R
Acceso en línea:	https://doaj.org/article/7356671095c745fe88a55c6eb47861ad
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spelling	oai:doaj.org-article:7356671095c745fe88a55c6eb47861ad2021-12-05T14:10:43ZComposite polyelectrolyte multilayers for biofunctionalization of medical devices2364-550410.1515/cdbme-2020-3110https://doaj.org/article/7356671095c745fe88a55c6eb47861ad2020-09-01T00:00:00Zhttps://doi.org/10.1515/cdbme-2020-3110https://doaj.org/toc/2364-5504Polyelectrolyte multilayer coatings (PEM) are prepared by alternative layer-by-layer deposition of cationic and anionic polyelectrolyte monolayers on charged surfaces. The thickness of the coatings ranges from nm to few μm. Their properties such as roughness, stiffness, surface charge and surface energy can be precisely tuned to fulfill different technical or biological requirements. The coating process is based on self-assembly of polyelectrolytes. Advantages of these coatings are their easy handling, no harsh chemistry and the possibility for coatings on complex geometries. The PEM coatings can be prepared from a variety of suitable polyelectrolytes. Their stability varies from very durable PEM coatings that are only soluble in strong solvents to quickly degradable, which may be applied as drug release system. One example of such a degradable PEM system is the one based on the polyelectrolyte pair Hyaluronan (HA) and Chitosan (CHI). These biopolymers originate from natural sources and show low toxicity towards human cells. However, HA/CHI multilayers shows only weak adhesiveness for human umbilical vein endothelial cells (HUVEC). In this article, we summarize our approaches to enhance the HA/CHI multilayer by incorporation of a non-polymer substance -graphene oxide- to improve the cell adhesion and keep such properties as low cytotoxicity and biodegradability. Different approaches for incorporation of graphene oxide were performed and the cellular adhesion was tested by metabolic assay.Rudt AlexanderAndreeva Tonya D.Krastev RumenTaneva Stefka G.De Gruyterarticlepolyelectrolyte multilayersbiocompatibilitycell adhesioncomposite filmsgraphene oxideMedicineRENCurrent Directions in Biomedical Engineering, Vol 6, Iss 3, Pp 426-429 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyelectrolyte multilayers biocompatibility cell adhesion composite films graphene oxide Medicine R
spellingShingle	polyelectrolyte multilayers biocompatibility cell adhesion composite films graphene oxide Medicine R Rudt Alexander Andreeva Tonya D. Krastev Rumen Taneva Stefka G. Composite polyelectrolyte multilayers for biofunctionalization of medical devices
description	Polyelectrolyte multilayer coatings (PEM) are prepared by alternative layer-by-layer deposition of cationic and anionic polyelectrolyte monolayers on charged surfaces. The thickness of the coatings ranges from nm to few μm. Their properties such as roughness, stiffness, surface charge and surface energy can be precisely tuned to fulfill different technical or biological requirements. The coating process is based on self-assembly of polyelectrolytes. Advantages of these coatings are their easy handling, no harsh chemistry and the possibility for coatings on complex geometries. The PEM coatings can be prepared from a variety of suitable polyelectrolytes. Their stability varies from very durable PEM coatings that are only soluble in strong solvents to quickly degradable, which may be applied as drug release system. One example of such a degradable PEM system is the one based on the polyelectrolyte pair Hyaluronan (HA) and Chitosan (CHI). These biopolymers originate from natural sources and show low toxicity towards human cells. However, HA/CHI multilayers shows only weak adhesiveness for human umbilical vein endothelial cells (HUVEC). In this article, we summarize our approaches to enhance the HA/CHI multilayer by incorporation of a non-polymer substance -graphene oxide- to improve the cell adhesion and keep such properties as low cytotoxicity and biodegradability. Different approaches for incorporation of graphene oxide were performed and the cellular adhesion was tested by metabolic assay.
format	article
author	Rudt Alexander Andreeva Tonya D. Krastev Rumen Taneva Stefka G.
author_facet	Rudt Alexander Andreeva Tonya D. Krastev Rumen Taneva Stefka G.
author_sort	Rudt Alexander
title	Composite polyelectrolyte multilayers for biofunctionalization of medical devices
title_short	Composite polyelectrolyte multilayers for biofunctionalization of medical devices
title_full	Composite polyelectrolyte multilayers for biofunctionalization of medical devices
title_fullStr	Composite polyelectrolyte multilayers for biofunctionalization of medical devices
title_full_unstemmed	Composite polyelectrolyte multilayers for biofunctionalization of medical devices
title_sort	composite polyelectrolyte multilayers for biofunctionalization of medical devices
publisher	De Gruyter
publishDate	2020
url	https://doaj.org/article/7356671095c745fe88a55c6eb47861ad
work_keys_str_mv	AT rudtalexander compositepolyelectrolytemultilayersforbiofunctionalizationofmedicaldevices AT andreevatonyad compositepolyelectrolytemultilayersforbiofunctionalizationofmedicaldevices AT krastevrumen compositepolyelectrolytemultilayersforbiofunctionalizationofmedicaldevices AT tanevastefkag compositepolyelectrolytemultilayersforbiofunctionalizationofmedicaldevices
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Composite polyelectrolyte multilayers for biofunctionalization of medical devices

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