Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry

Isobaric ions having the same mass-to-charge ratio cannot be separately identified by mass spectrometry (MS) alone, but this limitation can be overcome by using hydrogen–deuterium exchange (HDX) in microdroplets. Because isobaric ions may contain a varied number of exchangeable sites and different t...

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Autores principales:	Xiaowei Song, Jia Li, Mohammad Mofidfar, Richard N. Zare
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hydrogen-deuterium exchange isobaric ions ambient ionization mass spectrometry microdroplets Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/735dd03740714895acb0bd1e5a5ec44f
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spelling	oai:doaj.org-article:735dd03740714895acb0bd1e5a5ec44f2021-11-25T18:20:29ZDistinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry10.3390/metabo111107282218-1989https://doaj.org/article/735dd03740714895acb0bd1e5a5ec44f2021-10-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/728https://doaj.org/toc/2218-1989Isobaric ions having the same mass-to-charge ratio cannot be separately identified by mass spectrometry (MS) alone, but this limitation can be overcome by using hydrogen–deuterium exchange (HDX) in microdroplets. Because isobaric ions may contain a varied number of exchangeable sites and different types of functional groups, each one produces a unique MS spectral pattern after droplet spray HDX without the need for MS/MS experiments or introduction of ion mobility measurements. As an example of the power of this approach, isobaric ions in urinary metabolic profiles are identified and used to distinguish between healthy individuals and those having bladder cancer.Xiaowei SongJia LiMohammad MofidfarRichard N. ZareMDPI AGarticlehydrogen-deuterium exchangeisobaric ionsambient ionization mass spectrometrymicrodropletsMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 728, p 728 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hydrogen-deuterium exchange isobaric ions ambient ionization mass spectrometry microdroplets Microbiology QR1-502
spellingShingle	hydrogen-deuterium exchange isobaric ions ambient ionization mass spectrometry microdroplets Microbiology QR1-502 Xiaowei Song Jia Li Mohammad Mofidfar Richard N. Zare Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry
description	Isobaric ions having the same mass-to-charge ratio cannot be separately identified by mass spectrometry (MS) alone, but this limitation can be overcome by using hydrogen–deuterium exchange (HDX) in microdroplets. Because isobaric ions may contain a varied number of exchangeable sites and different types of functional groups, each one produces a unique MS spectral pattern after droplet spray HDX without the need for MS/MS experiments or introduction of ion mobility measurements. As an example of the power of this approach, isobaric ions in urinary metabolic profiles are identified and used to distinguish between healthy individuals and those having bladder cancer.
format	article
author	Xiaowei Song Jia Li Mohammad Mofidfar Richard N. Zare
author_facet	Xiaowei Song Jia Li Mohammad Mofidfar Richard N. Zare
author_sort	Xiaowei Song
title	Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry
title_short	Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry
title_full	Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry
title_fullStr	Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry
title_full_unstemmed	Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry
title_sort	distinguishing between isobaric ions using microdroplet hydrogen–deuterium exchange mass spectrometry
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/735dd03740714895acb0bd1e5a5ec44f
work_keys_str_mv	AT xiaoweisong distinguishingbetweenisobaricionsusingmicrodroplethydrogendeuteriumexchangemassspectrometry AT jiali distinguishingbetweenisobaricionsusingmicrodroplethydrogendeuteriumexchangemassspectrometry AT mohammadmofidfar distinguishingbetweenisobaricionsusingmicrodroplethydrogendeuteriumexchangemassspectrometry AT richardnzare distinguishingbetweenisobaricionsusingmicrodroplethydrogendeuteriumexchangemassspectrometry
_version_	1718411326868422656

Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry

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