Preparation and evaluation of ofloxacin-loaded palmitic acid solid lipid nanoparticles

Shuyu Xie, Luyan Zhu, Zhao Dong, Yan Wang, Xiaofang Wang, WenZhong ZhouDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing, People&rsquo;s Republic of ChinaAbstract: The purpose of this study was to use solid lipid nanoparticle...

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Autores principales:	Shuyu Xie, Luyan Zhu, Zhao Dong, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7366b86c4c6043c6970a56cf11dc4580
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spelling	oai:doaj.org-article:7366b86c4c6043c6970a56cf11dc45802021-12-02T00:35:30ZPreparation and evaluation of ofloxacin-loaded palmitic acid solid lipid nanoparticles1176-91141178-2013https://doaj.org/article/7366b86c4c6043c6970a56cf11dc45802011-03-01T00:00:00Zhttp://www.dovepress.com/preparation-and-evaluation-of-ofloxacin-loaded-palmitic-acid-solid-lip-a6705https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Shuyu Xie, Luyan Zhu, Zhao Dong, Yan Wang, Xiaofang Wang, WenZhong ZhouDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing, People&rsquo;s Republic of ChinaAbstract: The purpose of this study was to use solid lipid nanoparticles (SLN) to improve the pharmacological activity of ofloxacin. Ofloxacin-loaded SLN were prepared using palmitic acid as lipid matrix and poly vinyl alcohol (PVA) as emulsifier by a hot homogenization and ultrasonication method. The physicochemical characteristics of SLN were investigated by optical microscope, scanning electron microscopy, and photon correlation spectroscopy. Pharmacokinetics was studied after oral administration in mice. In vitro antibacterial activity and in vivo antibacterial efficacy of the SLN were investigated using minimal inhibitory concentrations (MIC) and a mouse protection model. The results demonstrated that the encapsulation efficiency, loading capacity, diameter, polydispersivity index, and zeta potential of the nanoparticles were 41.36% &plusmn; 1.50%, 4.40% &plusmn; 0.16%, 156.33 &plusmn; 7.51 nm, 0.26 &plusmn; 0.04, and &ndash;22.70 &plusmn; 1.40 mv, respectively. The SLN showed sustained release and enhanced antibacterial activity in vitro. Pharmacokinetic results demonstrated that SLN increased the bioavailability of ofloxacin by 2.27-fold, and extended the mean residence time of the drug from 10.50 to 43.44 hours. Single oral administrations of ofloxacin-loaded nanoparticles at 3 drug doses, 5 mg/kg, 10 mg/kg, and 20 mg/kg, all produced higher survival rates of lethal infected mice compared with native ofloxacin. These results indicate that SLN might be a promising delivery system to enhance the pharmacological activity of ofloxacin.Keywords: ofloxacin, pharmacological activity, solid lipid nanoparticles, antibacterial activity Shuyu XieLuyan ZhuZhao Donget alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 547-555 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Shuyu Xie Luyan Zhu Zhao Dong et al Preparation and evaluation of ofloxacin-loaded palmitic acid solid lipid nanoparticles
description	Shuyu Xie, Luyan Zhu, Zhao Dong, Yan Wang, Xiaofang Wang, WenZhong ZhouDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing, People&rsquo;s Republic of ChinaAbstract: The purpose of this study was to use solid lipid nanoparticles (SLN) to improve the pharmacological activity of ofloxacin. Ofloxacin-loaded SLN were prepared using palmitic acid as lipid matrix and poly vinyl alcohol (PVA) as emulsifier by a hot homogenization and ultrasonication method. The physicochemical characteristics of SLN were investigated by optical microscope, scanning electron microscopy, and photon correlation spectroscopy. Pharmacokinetics was studied after oral administration in mice. In vitro antibacterial activity and in vivo antibacterial efficacy of the SLN were investigated using minimal inhibitory concentrations (MIC) and a mouse protection model. The results demonstrated that the encapsulation efficiency, loading capacity, diameter, polydispersivity index, and zeta potential of the nanoparticles were 41.36% &plusmn; 1.50%, 4.40% &plusmn; 0.16%, 156.33 &plusmn; 7.51 nm, 0.26 &plusmn; 0.04, and &ndash;22.70 &plusmn; 1.40 mv, respectively. The SLN showed sustained release and enhanced antibacterial activity in vitro. Pharmacokinetic results demonstrated that SLN increased the bioavailability of ofloxacin by 2.27-fold, and extended the mean residence time of the drug from 10.50 to 43.44 hours. Single oral administrations of ofloxacin-loaded nanoparticles at 3 drug doses, 5 mg/kg, 10 mg/kg, and 20 mg/kg, all produced higher survival rates of lethal infected mice compared with native ofloxacin. These results indicate that SLN might be a promising delivery system to enhance the pharmacological activity of ofloxacin.Keywords: ofloxacin, pharmacological activity, solid lipid nanoparticles, antibacterial activity
format	article
author	Shuyu Xie Luyan Zhu Zhao Dong et al
author_facet	Shuyu Xie Luyan Zhu Zhao Dong et al
author_sort	Shuyu Xie
title	Preparation and evaluation of ofloxacin-loaded palmitic acid solid lipid nanoparticles
title_short	Preparation and evaluation of ofloxacin-loaded palmitic acid solid lipid nanoparticles
title_full	Preparation and evaluation of ofloxacin-loaded palmitic acid solid lipid nanoparticles
title_fullStr	Preparation and evaluation of ofloxacin-loaded palmitic acid solid lipid nanoparticles
title_full_unstemmed	Preparation and evaluation of ofloxacin-loaded palmitic acid solid lipid nanoparticles
title_sort	preparation and evaluation of ofloxacin-loaded palmitic acid solid lipid nanoparticles
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/7366b86c4c6043c6970a56cf11dc4580
work_keys_str_mv	AT shuyuxie preparationandevaluationofofloxacinloadedpalmiticacidsolidlipidnanoparticles AT luyanzhu preparationandevaluationofofloxacinloadedpalmiticacidsolidlipidnanoparticles AT zhaodong preparationandevaluationofofloxacinloadedpalmiticacidsolidlipidnanoparticles AT etal preparationandevaluationofofloxacinloadedpalmiticacidsolidlipidnanoparticles
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Preparation and evaluation of ofloxacin-loaded palmitic acid solid lipid nanoparticles

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