Ultraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles

Saravanan Govindaraju,1,3 Mohankandhasamy Ramasamy,1 Rengarajan Baskaran,2 Sang Jung Ahn,3,4 Kyusik Yun1 1Department of Bionanotechnology, Gachon University, Gyeonggi-do, 2College of Pharmacy, Gachon University, Incheon, 3Centre for Advanced Instrumentation, Korea Research Institute of Standard and...

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Autores principales:	Govindaraju S, Ramasamy M, Baskaran R, Ahn SJ, Yun KS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/73682e46d93a4ee69305f2c8f405a8a7
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spelling	oai:doaj.org-article:73682e46d93a4ee69305f2c8f405a8a72021-12-02T05:04:17ZUltraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles1178-2013https://doaj.org/article/73682e46d93a4ee69305f2c8f405a8a72015-08-01T00:00:00Zhttp://www.dovepress.com/ultraviolet-light-and-laser-irradiation-enhances-the-antibacterial-act-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Saravanan Govindaraju,1,3 Mohankandhasamy Ramasamy,1 Rengarajan Baskaran,2 Sang Jung Ahn,3,4 Kyusik Yun1 1Department of Bionanotechnology, Gachon University, Gyeonggi-do, 2College of Pharmacy, Gachon University, Incheon, 3Centre for Advanced Instrumentation, Korea Research Institute of Standard and Science, University of Science and Technology, Daejeon, Republic of Korea; 4Major of Nano Science, University of Science and Technology, Daejeon, Republic of Korea Abstract: Here we report a novel method for the synthesis of glucosamine-functionalized gold nanoparticles (GlcN-AuNPs) using biocompatible and biodegradable glucosamine for antibacterial activity. GlcN-AuNPs were prepared using different concentrations of glucosamine. The synthesized AuNPs were characterized for surface plasmon resonance, surface morphology, fluorescence spectroscopy, and antibacterial activity. The minimum inhibitory concentrations (MICs) of the AuNPs, GlcN-AuNPs, and GlcN-AuNPs when irradiated by ultraviolet light and laser were investigated and compared with the MIC of standard kanamycin using Escherichia coli by the microdilution method. Laser-irradiated GlcN-AuNPs exhibited significant bactericidal activity against E. coli. Flow cytometry and fluorescence microscopic analysis supported the cell death mechanism in the presence of GlcN-AuNP-treated bacteria. Further, morphological changes in E. coli after laser treatment were investigated using atomic force microscopy and transmission electron microscopy. The overall results of this study suggest that the prepared nanoparticles have potential as a potent antibacterial agent for the treatment of a wide range of disease-causing bacteria. Keywords: gold nanoparticles, glucosamine, light irradiation, antibacterial activity, bacterial morphologyGovindaraju SRamasamy MBaskaran RAhn SJYun KSDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss Special Issue on diverse applications in Nano-Theranostics, Pp 67-78 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Govindaraju S Ramasamy M Baskaran R Ahn SJ Yun KS Ultraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles
description	Saravanan Govindaraju,1,3 Mohankandhasamy Ramasamy,1 Rengarajan Baskaran,2 Sang Jung Ahn,3,4 Kyusik Yun1 1Department of Bionanotechnology, Gachon University, Gyeonggi-do, 2College of Pharmacy, Gachon University, Incheon, 3Centre for Advanced Instrumentation, Korea Research Institute of Standard and Science, University of Science and Technology, Daejeon, Republic of Korea; 4Major of Nano Science, University of Science and Technology, Daejeon, Republic of Korea Abstract: Here we report a novel method for the synthesis of glucosamine-functionalized gold nanoparticles (GlcN-AuNPs) using biocompatible and biodegradable glucosamine for antibacterial activity. GlcN-AuNPs were prepared using different concentrations of glucosamine. The synthesized AuNPs were characterized for surface plasmon resonance, surface morphology, fluorescence spectroscopy, and antibacterial activity. The minimum inhibitory concentrations (MICs) of the AuNPs, GlcN-AuNPs, and GlcN-AuNPs when irradiated by ultraviolet light and laser were investigated and compared with the MIC of standard kanamycin using Escherichia coli by the microdilution method. Laser-irradiated GlcN-AuNPs exhibited significant bactericidal activity against E. coli. Flow cytometry and fluorescence microscopic analysis supported the cell death mechanism in the presence of GlcN-AuNP-treated bacteria. Further, morphological changes in E. coli after laser treatment were investigated using atomic force microscopy and transmission electron microscopy. The overall results of this study suggest that the prepared nanoparticles have potential as a potent antibacterial agent for the treatment of a wide range of disease-causing bacteria. Keywords: gold nanoparticles, glucosamine, light irradiation, antibacterial activity, bacterial morphology
format	article
author	Govindaraju S Ramasamy M Baskaran R Ahn SJ Yun KS
author_facet	Govindaraju S Ramasamy M Baskaran R Ahn SJ Yun KS
author_sort	Govindaraju S
title	Ultraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles
title_short	Ultraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles
title_full	Ultraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles
title_fullStr	Ultraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles
title_full_unstemmed	Ultraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles
title_sort	ultraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/73682e46d93a4ee69305f2c8f405a8a7
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_version_	1718400658131910656

Ultraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles

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