Cholinergic modulation of sensory processing in awake mouse cortex

Abstract Cholinergic modulation of brain activity is fundamental for awareness and conscious sensorimotor behaviours, but deciphering the timing and significance of acetylcholine actions for these behaviours is challenging. The widespread nature of cholinergic projections to the cortex means that ne...

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Autores principales:	Javier Jimenez-Martin, Daniil Potapov, Kay Potapov, Thomas Knöpfel, Ruth M. Empson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/73730454205d4df7a402ff43b1062d0f
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spelling	oai:doaj.org-article:73730454205d4df7a402ff43b1062d0f2021-12-02T16:38:24ZCholinergic modulation of sensory processing in awake mouse cortex10.1038/s41598-021-96696-82045-2322https://doaj.org/article/73730454205d4df7a402ff43b1062d0f2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96696-8https://doaj.org/toc/2045-2322Abstract Cholinergic modulation of brain activity is fundamental for awareness and conscious sensorimotor behaviours, but deciphering the timing and significance of acetylcholine actions for these behaviours is challenging. The widespread nature of cholinergic projections to the cortex means that new insights require access to specific neuronal populations, and on a time-scale that matches behaviourally relevant cholinergic actions. Here, we use fast, voltage imaging of L2/3 cortical pyramidal neurons exclusively expressing the genetically-encoded voltage indicator Butterfly 1.2, in awake, head-fixed mice, receiving sensory stimulation, whilst manipulating the cholinergic system. Altering muscarinic acetylcholine function re-shaped sensory-evoked fast depolarisation and subsequent slow hyperpolarisation of L2/3 pyramidal neurons. A consequence of this re-shaping was disrupted adaptation of the sensory-evoked responses, suggesting a critical role for acetylcholine during sensory discrimination behaviour. Our findings provide new insights into how the cortex processes sensory information and how loss of acetylcholine, for example in Alzheimer’s Disease, disrupts sensory behaviours.Javier Jimenez-MartinDaniil PotapovKay PotapovThomas KnöpfelRuth M. EmpsonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-20 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Javier Jimenez-Martin Daniil Potapov Kay Potapov Thomas Knöpfel Ruth M. Empson Cholinergic modulation of sensory processing in awake mouse cortex
description	Abstract Cholinergic modulation of brain activity is fundamental for awareness and conscious sensorimotor behaviours, but deciphering the timing and significance of acetylcholine actions for these behaviours is challenging. The widespread nature of cholinergic projections to the cortex means that new insights require access to specific neuronal populations, and on a time-scale that matches behaviourally relevant cholinergic actions. Here, we use fast, voltage imaging of L2/3 cortical pyramidal neurons exclusively expressing the genetically-encoded voltage indicator Butterfly 1.2, in awake, head-fixed mice, receiving sensory stimulation, whilst manipulating the cholinergic system. Altering muscarinic acetylcholine function re-shaped sensory-evoked fast depolarisation and subsequent slow hyperpolarisation of L2/3 pyramidal neurons. A consequence of this re-shaping was disrupted adaptation of the sensory-evoked responses, suggesting a critical role for acetylcholine during sensory discrimination behaviour. Our findings provide new insights into how the cortex processes sensory information and how loss of acetylcholine, for example in Alzheimer’s Disease, disrupts sensory behaviours.
format	article
author	Javier Jimenez-Martin Daniil Potapov Kay Potapov Thomas Knöpfel Ruth M. Empson
author_facet	Javier Jimenez-Martin Daniil Potapov Kay Potapov Thomas Knöpfel Ruth M. Empson
author_sort	Javier Jimenez-Martin
title	Cholinergic modulation of sensory processing in awake mouse cortex
title_short	Cholinergic modulation of sensory processing in awake mouse cortex
title_full	Cholinergic modulation of sensory processing in awake mouse cortex
title_fullStr	Cholinergic modulation of sensory processing in awake mouse cortex
title_full_unstemmed	Cholinergic modulation of sensory processing in awake mouse cortex
title_sort	cholinergic modulation of sensory processing in awake mouse cortex
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/73730454205d4df7a402ff43b1062d0f
work_keys_str_mv	AT javierjimenezmartin cholinergicmodulationofsensoryprocessinginawakemousecortex AT daniilpotapov cholinergicmodulationofsensoryprocessinginawakemousecortex AT kaypotapov cholinergicmodulationofsensoryprocessinginawakemousecortex AT thomasknopfel cholinergicmodulationofsensoryprocessinginawakemousecortex AT ruthmempson cholinergicmodulationofsensoryprocessinginawakemousecortex
_version_	1718383580325871616

Cholinergic modulation of sensory processing in awake mouse cortex

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