Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration

Abstract Prolactin (PRL) hormone functions as a pleiotropic cytokine with a protective role in the retina. We recently identified by transcriptome profiling that PRL is one of the most highly upregulated mRNAs in the retinas of mutant rcd1 (PDE6B) and xlpra2 (RPGR) dogs at advanced stages of photore...

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Autores principales:	Raghavi Sudharsan, Leonardo Murgiano, Hsin-Yao Tang, Timothy W. Olsen, Venkata R. M. Chavali, Gustavo D. Aguirre, William A. Beltran
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7374429b174746bfb853812f0123472b
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spelling	oai:doaj.org-article:7374429b174746bfb853812f0123472b2021-12-02T15:22:58ZShort prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration10.1038/s41598-020-80691-62045-2322https://doaj.org/article/7374429b174746bfb853812f0123472b2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80691-6https://doaj.org/toc/2045-2322Abstract Prolactin (PRL) hormone functions as a pleiotropic cytokine with a protective role in the retina. We recently identified by transcriptome profiling that PRL is one of the most highly upregulated mRNAs in the retinas of mutant rcd1 (PDE6B) and xlpra2 (RPGR) dogs at advanced stages of photoreceptor disease. In the present study, we have identified the expression of a short PRL isoform that lacks exon 1 in canine retinas and analyzed the time-course of expression and localization of this isoform in the retinas of these two models. Using laser capture microdissection to isolate RNA from each of the retinal cellular layers, we found by qPCR that this short PRL isoform is expressed in photoreceptors of degenerating retinas. We confirmed by in situ hybridization that its expression is localized to the outer nuclear layer and begins shortly after the onset of disease at the time of peak photoreceptor cell death in both models. PRL protein was also detected only in mutant dog retinas. Our results call for further investigations into the role of this novel PRL isoform in retinal degeneration.Raghavi SudharsanLeonardo MurgianoHsin-Yao TangTimothy W. OlsenVenkata R. M. ChavaliGustavo D. AguirreWilliam A. BeltranNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Raghavi Sudharsan Leonardo Murgiano Hsin-Yao Tang Timothy W. Olsen Venkata R. M. Chavali Gustavo D. Aguirre William A. Beltran Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration
description	Abstract Prolactin (PRL) hormone functions as a pleiotropic cytokine with a protective role in the retina. We recently identified by transcriptome profiling that PRL is one of the most highly upregulated mRNAs in the retinas of mutant rcd1 (PDE6B) and xlpra2 (RPGR) dogs at advanced stages of photoreceptor disease. In the present study, we have identified the expression of a short PRL isoform that lacks exon 1 in canine retinas and analyzed the time-course of expression and localization of this isoform in the retinas of these two models. Using laser capture microdissection to isolate RNA from each of the retinal cellular layers, we found by qPCR that this short PRL isoform is expressed in photoreceptors of degenerating retinas. We confirmed by in situ hybridization that its expression is localized to the outer nuclear layer and begins shortly after the onset of disease at the time of peak photoreceptor cell death in both models. PRL protein was also detected only in mutant dog retinas. Our results call for further investigations into the role of this novel PRL isoform in retinal degeneration.
format	article
author	Raghavi Sudharsan Leonardo Murgiano Hsin-Yao Tang Timothy W. Olsen Venkata R. M. Chavali Gustavo D. Aguirre William A. Beltran
author_facet	Raghavi Sudharsan Leonardo Murgiano Hsin-Yao Tang Timothy W. Olsen Venkata R. M. Chavali Gustavo D. Aguirre William A. Beltran
author_sort	Raghavi Sudharsan
title	Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration
title_short	Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration
title_full	Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration
title_fullStr	Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration
title_full_unstemmed	Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration
title_sort	short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7374429b174746bfb853812f0123472b
work_keys_str_mv	AT raghavisudharsan shortprolactinisoformsareexpressedinphotoreceptorsofcanineretinasundergoingretinaldegeneration AT leonardomurgiano shortprolactinisoformsareexpressedinphotoreceptorsofcanineretinasundergoingretinaldegeneration AT hsinyaotang shortprolactinisoformsareexpressedinphotoreceptorsofcanineretinasundergoingretinaldegeneration AT timothywolsen shortprolactinisoformsareexpressedinphotoreceptorsofcanineretinasundergoingretinaldegeneration AT venkatarmchavali shortprolactinisoformsareexpressedinphotoreceptorsofcanineretinasundergoingretinaldegeneration AT gustavodaguirre shortprolactinisoformsareexpressedinphotoreceptorsofcanineretinasundergoingretinaldegeneration AT williamabeltran shortprolactinisoformsareexpressedinphotoreceptorsofcanineretinasundergoingretinaldegeneration
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Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration

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