Identification of bacterial biofilm and the Staphylococcus aureus derived protease, staphopain, on the skin surface of patients with atopic dermatitis

Abstract Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by an impaired epidermal barrier, dysregulation of innate and adaptive immunity, and a high susceptibility to bacterial colonization and infection. In the present study, bacterial biofilm was visualized by electron...

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Autores principales:	Andreas Sonesson, Kornelia Przybyszewska, Sigrid Eriksson, Matthias Mörgelin, Sven Kjellström, Julia Davies, Jan Potempa, Artur Schmidtchen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7388ad00ff7e40f595be6ffc6f1163cc
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spelling	oai:doaj.org-article:7388ad00ff7e40f595be6ffc6f1163cc2021-12-02T11:53:01ZIdentification of bacterial biofilm and the Staphylococcus aureus derived protease, staphopain, on the skin surface of patients with atopic dermatitis10.1038/s41598-017-08046-22045-2322https://doaj.org/article/7388ad00ff7e40f595be6ffc6f1163cc2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08046-2https://doaj.org/toc/2045-2322Abstract Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by an impaired epidermal barrier, dysregulation of innate and adaptive immunity, and a high susceptibility to bacterial colonization and infection. In the present study, bacterial biofilm was visualized by electron microscopy at the surface of AD skin. Correspondingly, Staphylococcus aureus (S. aureus) isolates from lesional skin of patients with AD, produced a substantial amount of biofilm in vitro. S. aureus biofilms showed less susceptibility to killing by the antimicrobial peptide LL-37 when compared with results obtained using planktonic cells. Confocal microscopy analysis showed that LL-37 binds to the S. aureus biofilms. Immuno-gold staining of S. aureus biofilm of AD skin detected the S. aureus derived protease staphopain adjacent to the bacteria. In vitro, staphopain B degraded LL-37 into shorter peptide fragments. Further, LL-37 significantly inhibited S. aureus biofilm formation, but no such effects were observed for the degradation products. The data presented here provide novel information on staphopains present in S. aureus biofilms in vivo, and illustrate the complex interplay between biofilm and LL-37 in skin of AD patients, possibly leading to a disturbed host defense, which facilitates bacterial persistence.Andreas SonessonKornelia PrzybyszewskaSigrid ErikssonMatthias MörgelinSven KjellströmJulia DaviesJan PotempaArtur SchmidtchenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andreas Sonesson Kornelia Przybyszewska Sigrid Eriksson Matthias Mörgelin Sven Kjellström Julia Davies Jan Potempa Artur Schmidtchen Identification of bacterial biofilm and the Staphylococcus aureus derived protease, staphopain, on the skin surface of patients with atopic dermatitis
description	Abstract Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by an impaired epidermal barrier, dysregulation of innate and adaptive immunity, and a high susceptibility to bacterial colonization and infection. In the present study, bacterial biofilm was visualized by electron microscopy at the surface of AD skin. Correspondingly, Staphylococcus aureus (S. aureus) isolates from lesional skin of patients with AD, produced a substantial amount of biofilm in vitro. S. aureus biofilms showed less susceptibility to killing by the antimicrobial peptide LL-37 when compared with results obtained using planktonic cells. Confocal microscopy analysis showed that LL-37 binds to the S. aureus biofilms. Immuno-gold staining of S. aureus biofilm of AD skin detected the S. aureus derived protease staphopain adjacent to the bacteria. In vitro, staphopain B degraded LL-37 into shorter peptide fragments. Further, LL-37 significantly inhibited S. aureus biofilm formation, but no such effects were observed for the degradation products. The data presented here provide novel information on staphopains present in S. aureus biofilms in vivo, and illustrate the complex interplay between biofilm and LL-37 in skin of AD patients, possibly leading to a disturbed host defense, which facilitates bacterial persistence.
format	article
author	Andreas Sonesson Kornelia Przybyszewska Sigrid Eriksson Matthias Mörgelin Sven Kjellström Julia Davies Jan Potempa Artur Schmidtchen
author_facet	Andreas Sonesson Kornelia Przybyszewska Sigrid Eriksson Matthias Mörgelin Sven Kjellström Julia Davies Jan Potempa Artur Schmidtchen
author_sort	Andreas Sonesson
title	Identification of bacterial biofilm and the Staphylococcus aureus derived protease, staphopain, on the skin surface of patients with atopic dermatitis
title_short	Identification of bacterial biofilm and the Staphylococcus aureus derived protease, staphopain, on the skin surface of patients with atopic dermatitis
title_full	Identification of bacterial biofilm and the Staphylococcus aureus derived protease, staphopain, on the skin surface of patients with atopic dermatitis
title_fullStr	Identification of bacterial biofilm and the Staphylococcus aureus derived protease, staphopain, on the skin surface of patients with atopic dermatitis
title_full_unstemmed	Identification of bacterial biofilm and the Staphylococcus aureus derived protease, staphopain, on the skin surface of patients with atopic dermatitis
title_sort	identification of bacterial biofilm and the staphylococcus aureus derived protease, staphopain, on the skin surface of patients with atopic dermatitis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7388ad00ff7e40f595be6ffc6f1163cc
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Identification of bacterial biofilm and the Staphylococcus aureus derived protease, staphopain, on the skin surface of patients with atopic dermatitis

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