Metaproteomics: Much More than Measuring Gene Expression in Microbial Communities

ABSTRACT Metaproteomics is the large-scale identification and quantification of proteins from microbial communities and thus provides direct insight into the phenotypes of microorganisms on the molecular level. Initially, metaproteomics was mainly used to assess the “expressed” metabolism and physio...

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Autor principal:	Manuel Kleiner
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	LC-MS metagenomics metaproteomics metatranscriptomics microbiome microbiota Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/73924d680a094c3cb5a58085d976d9ca
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spelling	oai:doaj.org-article:73924d680a094c3cb5a58085d976d9ca2021-12-02T19:46:18ZMetaproteomics: Much More than Measuring Gene Expression in Microbial Communities10.1128/mSystems.00115-192379-5077https://doaj.org/article/73924d680a094c3cb5a58085d976d9ca2019-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00115-19https://doaj.org/toc/2379-5077ABSTRACT Metaproteomics is the large-scale identification and quantification of proteins from microbial communities and thus provides direct insight into the phenotypes of microorganisms on the molecular level. Initially, metaproteomics was mainly used to assess the “expressed” metabolism and physiology of microbial community members. However, recently developed metaproteomic tools allow quantification of per-species biomass to determine community structure, in situ carbon sources of community members, and the uptake of labeled substrates by community members. In this perspective, I provide a brief overview of the questions that we can currently address, as well as new metaproteomics-based approaches that we and others are developing to address even more questions in the study of microbial communities and plant and animal microbiota. I also highlight some areas and technologies where I anticipate developments and potentially major breakthroughs in the next 5 years and beyond.Manuel KleinerAmerican Society for MicrobiologyarticleLC-MSmetagenomicsmetaproteomicsmetatranscriptomicsmicrobiomemicrobiotaMicrobiologyQR1-502ENmSystems, Vol 4, Iss 3 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	LC-MS metagenomics metaproteomics metatranscriptomics microbiome microbiota Microbiology QR1-502
spellingShingle	LC-MS metagenomics metaproteomics metatranscriptomics microbiome microbiota Microbiology QR1-502 Manuel Kleiner Metaproteomics: Much More than Measuring Gene Expression in Microbial Communities
description	ABSTRACT Metaproteomics is the large-scale identification and quantification of proteins from microbial communities and thus provides direct insight into the phenotypes of microorganisms on the molecular level. Initially, metaproteomics was mainly used to assess the “expressed” metabolism and physiology of microbial community members. However, recently developed metaproteomic tools allow quantification of per-species biomass to determine community structure, in situ carbon sources of community members, and the uptake of labeled substrates by community members. In this perspective, I provide a brief overview of the questions that we can currently address, as well as new metaproteomics-based approaches that we and others are developing to address even more questions in the study of microbial communities and plant and animal microbiota. I also highlight some areas and technologies where I anticipate developments and potentially major breakthroughs in the next 5 years and beyond.
format	article
author	Manuel Kleiner
author_facet	Manuel Kleiner
author_sort	Manuel Kleiner
title	Metaproteomics: Much More than Measuring Gene Expression in Microbial Communities
title_short	Metaproteomics: Much More than Measuring Gene Expression in Microbial Communities
title_full	Metaproteomics: Much More than Measuring Gene Expression in Microbial Communities
title_fullStr	Metaproteomics: Much More than Measuring Gene Expression in Microbial Communities
title_full_unstemmed	Metaproteomics: Much More than Measuring Gene Expression in Microbial Communities
title_sort	metaproteomics: much more than measuring gene expression in microbial communities
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/73924d680a094c3cb5a58085d976d9ca
work_keys_str_mv	AT manuelkleiner metaproteomicsmuchmorethanmeasuringgeneexpressioninmicrobialcommunities
_version_	1718376032022560768

Metaproteomics: Much More than Measuring Gene Expression in Microbial Communities

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