Naturalizing laboratory mice by housing in a farmyard-type habitat confers protection against colorectal carcinogenesis

Living in a farm environment in proximity to animals is associated with reduced risk of developing allergies and asthma, and has been suggested to protect against other diseases, such as inflammatory bowel disease and cancer. Despite epidemiological evidence, experimental disease models that recapit...

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Autores principales:	Henriette Arnesen, Thomas C. A. Hitch, Christina Steppeler, Mette Helen Bjørge Müller, Linn Emilie Knutsen, Gjermund Gunnes, Inga Leena Angell, Ida Ormaasen, Knut Rudi, Jan Erik Paulsen, Thomas Clavel, Harald Carlsen, Preben Boysen
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	gut microbiota feralized mice colorectal cancer farmyard-like habitat animal model naturalized mice short-chain fatty acids immunity Diseases of the digestive system. Gastroenterology RC799-869
Acceso en línea:	https://doaj.org/article/73929f4613834897a636e69c6c019117
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spelling	oai:doaj.org-article:73929f4613834897a636e69c6c0191172021-11-11T14:23:42ZNaturalizing laboratory mice by housing in a farmyard-type habitat confers protection against colorectal carcinogenesis1949-09761949-098410.1080/19490976.2021.1993581https://doaj.org/article/73929f4613834897a636e69c6c0191172021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/19490976.2021.1993581https://doaj.org/toc/1949-0976https://doaj.org/toc/1949-0984Living in a farm environment in proximity to animals is associated with reduced risk of developing allergies and asthma, and has been suggested to protect against other diseases, such as inflammatory bowel disease and cancer. Despite epidemiological evidence, experimental disease models that recapitulate such environments are needed to understand the underlying mechanisms. In this study, we show that feralizing conventional inbred mice by continuous exposure to a livestock farmyard-type environment conferred protection toward colorectal carcinogenesis. Two independent experimental approaches for colorectal cancer induction were used; spontaneous (Apc Min/+ mice on an A/J background) or chemical (AOM/DSS). In contrast to conventionally reared laboratory mice, the feralized mouse gut microbiota structure remained stable and resistant to mutagen- and colitis-induced neoplasia. Moreover, the feralized mice exhibited signs of a more mature immunophenotype, indicated by increased expression of NK and T-cell maturation markers, and a more potent IFN-γ response to stimuli. In our study, hygienically born and raised mice subsequently feralized post-weaning were protected to a similar level as life-long exposed mice, although the greatest effect was seen upon neonatal exposure. Collectively, we show protective implications of a farmyard-type environment on colorectal cancer development and demonstrate the utility of a novel animal modeling approach that recapitulates realistic disease responses in a naturalized mammal.Henriette ArnesenThomas C. A. HitchChristina SteppelerMette Helen Bjørge MüllerLinn Emilie KnutsenGjermund GunnesInga Leena AngellIda OrmaasenKnut RudiJan Erik PaulsenThomas ClavelHarald CarlsenPreben BoysenTaylor & Francis Grouparticlegut microbiotaferalized micecolorectal cancerfarmyard-like habitatanimal modelnaturalized miceshort-chain fatty acidsimmunityDiseases of the digestive system. GastroenterologyRC799-869ENGut Microbes, Vol 13, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gut microbiota feralized mice colorectal cancer farmyard-like habitat animal model naturalized mice short-chain fatty acids immunity Diseases of the digestive system. Gastroenterology RC799-869
spellingShingle	gut microbiota feralized mice colorectal cancer farmyard-like habitat animal model naturalized mice short-chain fatty acids immunity Diseases of the digestive system. Gastroenterology RC799-869 Henriette Arnesen Thomas C. A. Hitch Christina Steppeler Mette Helen Bjørge Müller Linn Emilie Knutsen Gjermund Gunnes Inga Leena Angell Ida Ormaasen Knut Rudi Jan Erik Paulsen Thomas Clavel Harald Carlsen Preben Boysen Naturalizing laboratory mice by housing in a farmyard-type habitat confers protection against colorectal carcinogenesis
description	Living in a farm environment in proximity to animals is associated with reduced risk of developing allergies and asthma, and has been suggested to protect against other diseases, such as inflammatory bowel disease and cancer. Despite epidemiological evidence, experimental disease models that recapitulate such environments are needed to understand the underlying mechanisms. In this study, we show that feralizing conventional inbred mice by continuous exposure to a livestock farmyard-type environment conferred protection toward colorectal carcinogenesis. Two independent experimental approaches for colorectal cancer induction were used; spontaneous (Apc Min/+ mice on an A/J background) or chemical (AOM/DSS). In contrast to conventionally reared laboratory mice, the feralized mouse gut microbiota structure remained stable and resistant to mutagen- and colitis-induced neoplasia. Moreover, the feralized mice exhibited signs of a more mature immunophenotype, indicated by increased expression of NK and T-cell maturation markers, and a more potent IFN-γ response to stimuli. In our study, hygienically born and raised mice subsequently feralized post-weaning were protected to a similar level as life-long exposed mice, although the greatest effect was seen upon neonatal exposure. Collectively, we show protective implications of a farmyard-type environment on colorectal cancer development and demonstrate the utility of a novel animal modeling approach that recapitulates realistic disease responses in a naturalized mammal.
format	article
author	Henriette Arnesen Thomas C. A. Hitch Christina Steppeler Mette Helen Bjørge Müller Linn Emilie Knutsen Gjermund Gunnes Inga Leena Angell Ida Ormaasen Knut Rudi Jan Erik Paulsen Thomas Clavel Harald Carlsen Preben Boysen
author_facet	Henriette Arnesen Thomas C. A. Hitch Christina Steppeler Mette Helen Bjørge Müller Linn Emilie Knutsen Gjermund Gunnes Inga Leena Angell Ida Ormaasen Knut Rudi Jan Erik Paulsen Thomas Clavel Harald Carlsen Preben Boysen
author_sort	Henriette Arnesen
title	Naturalizing laboratory mice by housing in a farmyard-type habitat confers protection against colorectal carcinogenesis
title_short	Naturalizing laboratory mice by housing in a farmyard-type habitat confers protection against colorectal carcinogenesis
title_full	Naturalizing laboratory mice by housing in a farmyard-type habitat confers protection against colorectal carcinogenesis
title_fullStr	Naturalizing laboratory mice by housing in a farmyard-type habitat confers protection against colorectal carcinogenesis
title_full_unstemmed	Naturalizing laboratory mice by housing in a farmyard-type habitat confers protection against colorectal carcinogenesis
title_sort	naturalizing laboratory mice by housing in a farmyard-type habitat confers protection against colorectal carcinogenesis
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/73929f4613834897a636e69c6c019117
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Naturalizing laboratory mice by housing in a farmyard-type habitat confers protection against colorectal carcinogenesis

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