Genetic diversity of staphylocoagulase genes (coa): insight into the evolution of variable chromosomal virulence factors in Staphylococcus aureus.

<h4>Background</h4>The production of staphylocoagulase (SC) causing the plasma coagulation is one of the important characteristics of Staphylococcus aureus. Although SCs have been classified into 10 serotypes based on the differences in the antigenicity, genetic bases for their diversiti...

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Autores principales:	Shinya Watanabe, Teruyo Ito, Takashi Sasaki, Shanshuang Li, Ikuo Uchiyama, Kozue Kishii, Ken Kikuchi, Robert Leo Skov, Keiichi Hiramatsu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7395517d403c44bfbf62b0b5a8ffe223
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spelling	oai:doaj.org-article:7395517d403c44bfbf62b0b5a8ffe2232021-11-25T06:22:27ZGenetic diversity of staphylocoagulase genes (coa): insight into the evolution of variable chromosomal virulence factors in Staphylococcus aureus.1932-620310.1371/journal.pone.0005714https://doaj.org/article/7395517d403c44bfbf62b0b5a8ffe2232009-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19492076/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The production of staphylocoagulase (SC) causing the plasma coagulation is one of the important characteristics of Staphylococcus aureus. Although SCs have been classified into 10 serotypes based on the differences in the antigenicity, genetic bases for their diversities and relatedness to chromosome types are poorly understood.<h4>Methodology/principal findings</h4>We compared the nucleotide sequences of 105 SC genes (coa), 59 of which were determined in this study. D1 regions, which contain prothrombin-activating and -binding domains and are presumed to be the binding site of each type-specific antiserum, were classified into twelve clusters having more than 90% nucleotide identities, resulting to create two novel SC types, XI and XII, in addition to extant 10 types. Nine of the twelve SC types were further subdivided into subtypes based on the differences of the D2 or the central regions. The phylogenetical relations of the D1 regions did not correlate exactly with either one of agr types and multilocus sequence types (STs). In addition, genetic analysis showed that recombination events have occurred in and around coa. So far tested, STs of 126 S. aureus strains correspond to the combination of SC type and agr type except for the cases of CC1 and CC8, which contained two and three different SC types, respectively.<h4>Conclusion</h4>The data suggested that the evolution of coa was not monophyletic in the species. Chromosomal recombination had occurred at coa and agr loci, resulting in the carriage of the combinations of allotypically different important virulence determinants in staphylococcal chromosome.Shinya WatanabeTeruyo ItoTakashi SasakiShanshuang LiIkuo UchiyamaKozue KishiiKen KikuchiRobert Leo SkovKeiichi HiramatsuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 5, p e5714 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shinya Watanabe Teruyo Ito Takashi Sasaki Shanshuang Li Ikuo Uchiyama Kozue Kishii Ken Kikuchi Robert Leo Skov Keiichi Hiramatsu Genetic diversity of staphylocoagulase genes (coa): insight into the evolution of variable chromosomal virulence factors in Staphylococcus aureus.
description	<h4>Background</h4>The production of staphylocoagulase (SC) causing the plasma coagulation is one of the important characteristics of Staphylococcus aureus. Although SCs have been classified into 10 serotypes based on the differences in the antigenicity, genetic bases for their diversities and relatedness to chromosome types are poorly understood.<h4>Methodology/principal findings</h4>We compared the nucleotide sequences of 105 SC genes (coa), 59 of which were determined in this study. D1 regions, which contain prothrombin-activating and -binding domains and are presumed to be the binding site of each type-specific antiserum, were classified into twelve clusters having more than 90% nucleotide identities, resulting to create two novel SC types, XI and XII, in addition to extant 10 types. Nine of the twelve SC types were further subdivided into subtypes based on the differences of the D2 or the central regions. The phylogenetical relations of the D1 regions did not correlate exactly with either one of agr types and multilocus sequence types (STs). In addition, genetic analysis showed that recombination events have occurred in and around coa. So far tested, STs of 126 S. aureus strains correspond to the combination of SC type and agr type except for the cases of CC1 and CC8, which contained two and three different SC types, respectively.<h4>Conclusion</h4>The data suggested that the evolution of coa was not monophyletic in the species. Chromosomal recombination had occurred at coa and agr loci, resulting in the carriage of the combinations of allotypically different important virulence determinants in staphylococcal chromosome.
format	article
author	Shinya Watanabe Teruyo Ito Takashi Sasaki Shanshuang Li Ikuo Uchiyama Kozue Kishii Ken Kikuchi Robert Leo Skov Keiichi Hiramatsu
author_facet	Shinya Watanabe Teruyo Ito Takashi Sasaki Shanshuang Li Ikuo Uchiyama Kozue Kishii Ken Kikuchi Robert Leo Skov Keiichi Hiramatsu
author_sort	Shinya Watanabe
title	Genetic diversity of staphylocoagulase genes (coa): insight into the evolution of variable chromosomal virulence factors in Staphylococcus aureus.
title_short	Genetic diversity of staphylocoagulase genes (coa): insight into the evolution of variable chromosomal virulence factors in Staphylococcus aureus.
title_full	Genetic diversity of staphylocoagulase genes (coa): insight into the evolution of variable chromosomal virulence factors in Staphylococcus aureus.
title_fullStr	Genetic diversity of staphylocoagulase genes (coa): insight into the evolution of variable chromosomal virulence factors in Staphylococcus aureus.
title_full_unstemmed	Genetic diversity of staphylocoagulase genes (coa): insight into the evolution of variable chromosomal virulence factors in Staphylococcus aureus.
title_sort	genetic diversity of staphylocoagulase genes (coa): insight into the evolution of variable chromosomal virulence factors in staphylococcus aureus.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/7395517d403c44bfbf62b0b5a8ffe223
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Genetic diversity of staphylocoagulase genes (coa): insight into the evolution of variable chromosomal virulence factors in Staphylococcus aureus.

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