A highly sensitive and selective viral protein detection method based on RNA oligonucleotide nanoparticle

Changhyun Roh1, Ho-Young Lee2, Sang-Eun Kim2, Sung-Kee Jo11Radiation Research Division for Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), Sinjeong-dong, Jeongeup, Jeonbuk, South Korea; 2Department of Nuclear Medicine, College of Medicin...

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Autores principales:	Changhyun Roh, Ho-Young Lee, Sang-Eun Kim, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/73969957c4314c88b3260048601608c3
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spelling	oai:doaj.org-article:73969957c4314c88b3260048601608c32021-12-02T01:33:11ZA highly sensitive and selective viral protein detection method based on RNA oligonucleotide nanoparticle1176-91141178-2013https://doaj.org/article/73969957c4314c88b3260048601608c32010-04-01T00:00:00Zhttp://www.dovepress.com/a-highly-sensitive-and-selective-viral-protein-detection-method-based--a4310https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Changhyun Roh1, Ho-Young Lee2, Sang-Eun Kim2, Sung-Kee Jo11Radiation Research Division for Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), Sinjeong-dong, Jeongeup, Jeonbuk, South Korea; 2Department of Nuclear Medicine, College of Medicine, Seoul National University, South KoreaAbstract: Globally, approximately 170 million people (representing approximately 3% of the population worldwide), are infected with hepatitis C virus (HCV) and at risk of serious liver disease, including chronic hepatitis. We propose a new quantum dots (QDs)-supported RNA oligonucleotide approach for the specific and sensitive detection of viral protein using a biochip. This method was developed by immobilizing a HCV nonstructural protein 5B (NS5B) on the surface of a glass chip via the formation of a covalent bond between an amine protein group and a ProLinkerTM glass chip. The QDs-supported RNA oligonucleotide was conjugated via an amide formation reaction from coupling of a 5&prime;-end-amine-modified RNA oligonucleotide on the surface of QDs displaying carboxyl groups via standard EDC coupling. The QDs-conjugated RNA oligonucleotide was interacted to immobilized viral protein NS5B on the biochip. The detection is based on the variation of signal of QDs-supported RNA oligonucleotide bound on an immobilized biochip. It was demonstrated that the value of the signal has a linear relationship with concentrations of the HCV NS5B viral protein in the 1 &mu;g mL-1 to 1 ng mL-1 range with a detection limit of 1 ng mL-1. The major advantages of this RNA-oligonucleotide nanoparticle assay are its good specificity, ease of performance, and ability to perform one-spot monitoring. The proposed method could be used as a general method of HCV detection and is expected to be applicable to other types of diseases as well.Keywords: hepatitis C virus, viral protein, RNA oligonucleotide, quantum dots, biochip Changhyun RohHo-Young LeeSang-Eun Kimet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 323-329 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Changhyun Roh Ho-Young Lee Sang-Eun Kim et al A highly sensitive and selective viral protein detection method based on RNA oligonucleotide nanoparticle
description	Changhyun Roh1, Ho-Young Lee2, Sang-Eun Kim2, Sung-Kee Jo11Radiation Research Division for Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), Sinjeong-dong, Jeongeup, Jeonbuk, South Korea; 2Department of Nuclear Medicine, College of Medicine, Seoul National University, South KoreaAbstract: Globally, approximately 170 million people (representing approximately 3% of the population worldwide), are infected with hepatitis C virus (HCV) and at risk of serious liver disease, including chronic hepatitis. We propose a new quantum dots (QDs)-supported RNA oligonucleotide approach for the specific and sensitive detection of viral protein using a biochip. This method was developed by immobilizing a HCV nonstructural protein 5B (NS5B) on the surface of a glass chip via the formation of a covalent bond between an amine protein group and a ProLinkerTM glass chip. The QDs-supported RNA oligonucleotide was conjugated via an amide formation reaction from coupling of a 5&prime;-end-amine-modified RNA oligonucleotide on the surface of QDs displaying carboxyl groups via standard EDC coupling. The QDs-conjugated RNA oligonucleotide was interacted to immobilized viral protein NS5B on the biochip. The detection is based on the variation of signal of QDs-supported RNA oligonucleotide bound on an immobilized biochip. It was demonstrated that the value of the signal has a linear relationship with concentrations of the HCV NS5B viral protein in the 1 &mu;g mL-1 to 1 ng mL-1 range with a detection limit of 1 ng mL-1. The major advantages of this RNA-oligonucleotide nanoparticle assay are its good specificity, ease of performance, and ability to perform one-spot monitoring. The proposed method could be used as a general method of HCV detection and is expected to be applicable to other types of diseases as well.Keywords: hepatitis C virus, viral protein, RNA oligonucleotide, quantum dots, biochip
format	article
author	Changhyun Roh Ho-Young Lee Sang-Eun Kim et al
author_facet	Changhyun Roh Ho-Young Lee Sang-Eun Kim et al
author_sort	Changhyun Roh
title	A highly sensitive and selective viral protein detection method based on RNA oligonucleotide nanoparticle
title_short	A highly sensitive and selective viral protein detection method based on RNA oligonucleotide nanoparticle
title_full	A highly sensitive and selective viral protein detection method based on RNA oligonucleotide nanoparticle
title_fullStr	A highly sensitive and selective viral protein detection method based on RNA oligonucleotide nanoparticle
title_full_unstemmed	A highly sensitive and selective viral protein detection method based on RNA oligonucleotide nanoparticle
title_sort	highly sensitive and selective viral protein detection method based on rna oligonucleotide nanoparticle
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/73969957c4314c88b3260048601608c3
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A highly sensitive and selective viral protein detection method based on RNA oligonucleotide nanoparticle

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