Taxus wallichiana var. chinensis (Pilg.) Florin Aqueous Extract Suppresses the Proliferation and Metastasis in Lung Carcinoma via JAK/STAT3 Signaling Pathway

As one of the most common neoplasms globally, lung cancer (LC) is the leading cause of cancer-related mortality. Recurrence and metastasis negatively influencing therapeutic efficacy and overall survival demand new strategies in LC treatment. The advantages of TCM are increasingly highlighted. In th...

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Autores principales:	Leitao Sun, Shuning Ding, Qi Luo, Peipei Wang, Xiao Yang, Linqin Wu, Yangfan Chen, Xueer Zheng, Hang Zhang, Li Yuan, Shanming Ruan, Changsheng Xie
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	lung cancer proliferation metastasis JAK/STAT3 signaling pathway Taxus wallichiana var. chinensis (Pilg.) Florin Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/73a0cd2e1a544800afded5e96fd0ce52
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spelling	oai:doaj.org-article:73a0cd2e1a544800afded5e96fd0ce522021-11-16T05:31:25ZTaxus wallichiana var. chinensis (Pilg.) Florin Aqueous Extract Suppresses the Proliferation and Metastasis in Lung Carcinoma via JAK/STAT3 Signaling Pathway1663-981210.3389/fphar.2021.736442https://doaj.org/article/73a0cd2e1a544800afded5e96fd0ce522021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.736442/fullhttps://doaj.org/toc/1663-9812As one of the most common neoplasms globally, lung cancer (LC) is the leading cause of cancer-related mortality. Recurrence and metastasis negatively influencing therapeutic efficacy and overall survival demand new strategies in LC treatment. The advantages of TCM are increasingly highlighted. In this study, we obtained the major chemical components and their ratios in the aqueous extract of Taxus wallichiana var. chinensis (Pilg.) Florin (AETW) by UPLC-Q/TOF-MS/MS detection. The CCK-8 assay revealed that AETW could selectively inhibit the growth of A549 and HCC827 cells in a dose-dependent manner with little effect on normal human lung cells. Moreover, both in vitro and in vivo experiments showed that AETW was able to suppress the capacities of cell migration and invasion and downregulate the EMT and the JAK/STAT3 signaling pathway. To further probe into the molecular mechanism, the overexpression of STAT3 was performed into LC cells with AETW treatment, which counteracted the inhibitory effect on malignant behaviors of A549 and HCC827 cells with the decline in the expressions of p-JAK and p-STAT3. Taken together, we propose that AETW may inhibit the proliferation and metastasis by inactivating the JAK/STAT3 axis.Leitao SunShuning DingQi LuoPeipei WangXiao YangLinqin WuYangfan ChenXueer ZhengHang ZhangLi YuanShanming RuanChangsheng XieFrontiers Media S.A.articlelung cancerproliferationmetastasisJAK/STAT3 signaling pathwayTaxus wallichiana var. chinensis (Pilg.) FlorinTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lung cancer proliferation metastasis JAK/STAT3 signaling pathway Taxus wallichiana var. chinensis (Pilg.) Florin Therapeutics. Pharmacology RM1-950
spellingShingle	lung cancer proliferation metastasis JAK/STAT3 signaling pathway Taxus wallichiana var. chinensis (Pilg.) Florin Therapeutics. Pharmacology RM1-950 Leitao Sun Shuning Ding Qi Luo Peipei Wang Xiao Yang Linqin Wu Yangfan Chen Xueer Zheng Hang Zhang Li Yuan Shanming Ruan Changsheng Xie Taxus wallichiana var. chinensis (Pilg.) Florin Aqueous Extract Suppresses the Proliferation and Metastasis in Lung Carcinoma via JAK/STAT3 Signaling Pathway
description	As one of the most common neoplasms globally, lung cancer (LC) is the leading cause of cancer-related mortality. Recurrence and metastasis negatively influencing therapeutic efficacy and overall survival demand new strategies in LC treatment. The advantages of TCM are increasingly highlighted. In this study, we obtained the major chemical components and their ratios in the aqueous extract of Taxus wallichiana var. chinensis (Pilg.) Florin (AETW) by UPLC-Q/TOF-MS/MS detection. The CCK-8 assay revealed that AETW could selectively inhibit the growth of A549 and HCC827 cells in a dose-dependent manner with little effect on normal human lung cells. Moreover, both in vitro and in vivo experiments showed that AETW was able to suppress the capacities of cell migration and invasion and downregulate the EMT and the JAK/STAT3 signaling pathway. To further probe into the molecular mechanism, the overexpression of STAT3 was performed into LC cells with AETW treatment, which counteracted the inhibitory effect on malignant behaviors of A549 and HCC827 cells with the decline in the expressions of p-JAK and p-STAT3. Taken together, we propose that AETW may inhibit the proliferation and metastasis by inactivating the JAK/STAT3 axis.
format	article
author	Leitao Sun Shuning Ding Qi Luo Peipei Wang Xiao Yang Linqin Wu Yangfan Chen Xueer Zheng Hang Zhang Li Yuan Shanming Ruan Changsheng Xie
author_facet	Leitao Sun Shuning Ding Qi Luo Peipei Wang Xiao Yang Linqin Wu Yangfan Chen Xueer Zheng Hang Zhang Li Yuan Shanming Ruan Changsheng Xie
author_sort	Leitao Sun
title	Taxus wallichiana var. chinensis (Pilg.) Florin Aqueous Extract Suppresses the Proliferation and Metastasis in Lung Carcinoma via JAK/STAT3 Signaling Pathway
title_short	Taxus wallichiana var. chinensis (Pilg.) Florin Aqueous Extract Suppresses the Proliferation and Metastasis in Lung Carcinoma via JAK/STAT3 Signaling Pathway
title_full	Taxus wallichiana var. chinensis (Pilg.) Florin Aqueous Extract Suppresses the Proliferation and Metastasis in Lung Carcinoma via JAK/STAT3 Signaling Pathway
title_fullStr	Taxus wallichiana var. chinensis (Pilg.) Florin Aqueous Extract Suppresses the Proliferation and Metastasis in Lung Carcinoma via JAK/STAT3 Signaling Pathway
title_full_unstemmed	Taxus wallichiana var. chinensis (Pilg.) Florin Aqueous Extract Suppresses the Proliferation and Metastasis in Lung Carcinoma via JAK/STAT3 Signaling Pathway
title_sort	taxus wallichiana var. chinensis (pilg.) florin aqueous extract suppresses the proliferation and metastasis in lung carcinoma via jak/stat3 signaling pathway
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/73a0cd2e1a544800afded5e96fd0ce52
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Taxus wallichiana var. chinensis (Pilg.) Florin Aqueous Extract Suppresses the Proliferation and Metastasis in Lung Carcinoma via JAK/STAT3 Signaling Pathway

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