Crosstalk between the chloroplast protein import and SUMO systems revealed through genetic and molecular investigation in Arabidopsis

The chloroplast proteome contains thousands of different proteins that are encoded by the nuclear genome. These proteins are imported into the chloroplast via the action of the TOC translocase and associated downstream systems. Our recent work has revealed that the stability of the TOC complex is dy...

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Autores principales:	Samuel James Watson, Na Li, Yiting Ye, Feijie Wu, Qihua Ling, R Paul Jarvis
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	chloroplast SUMO protein translocation TOC proteins chloroplast protein import chloroplast biogenesis Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/73a5eff73f614347bdc9a2b61ed299c5
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id	oai:doaj.org-article:73a5eff73f614347bdc9a2b61ed299c5
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spelling	oai:doaj.org-article:73a5eff73f614347bdc9a2b61ed299c52021-11-23T13:02:31ZCrosstalk between the chloroplast protein import and SUMO systems revealed through genetic and molecular investigation in Arabidopsis10.7554/eLife.609602050-084Xe60960https://doaj.org/article/73a5eff73f614347bdc9a2b61ed299c52021-09-01T00:00:00Zhttps://elifesciences.org/articles/60960https://doaj.org/toc/2050-084XThe chloroplast proteome contains thousands of different proteins that are encoded by the nuclear genome. These proteins are imported into the chloroplast via the action of the TOC translocase and associated downstream systems. Our recent work has revealed that the stability of the TOC complex is dynamically regulated by the ubiquitin-dependent chloroplast-associated protein degradation pathway. Here, we demonstrate that the TOC complex is also regulated by the small ubiquitin-like modifier (SUMO) system. Arabidopsis mutants representing almost the entire SUMO conjugation pathway can partially suppress the phenotype of ppi1, a pale-yellow mutant lacking the Toc33 protein. This suppression is linked to increased abundance of TOC proteins and improvements in chloroplast development. Moreover, data from molecular and biochemical experiments support a model in which the SUMO system directly regulates TOC protein stability. Thus, we have identified a regulatory link between the SUMO system and the chloroplast protein import machinery.Samuel James WatsonNa LiYiting YeFeijie WuQihua LingR Paul JarviseLife Sciences Publications LtdarticlechloroplastSUMOprotein translocationTOC proteinschloroplast protein importchloroplast biogenesisMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chloroplast SUMO protein translocation TOC proteins chloroplast protein import chloroplast biogenesis Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	chloroplast SUMO protein translocation TOC proteins chloroplast protein import chloroplast biogenesis Medicine R Science Q Biology (General) QH301-705.5 Samuel James Watson Na Li Yiting Ye Feijie Wu Qihua Ling R Paul Jarvis Crosstalk between the chloroplast protein import and SUMO systems revealed through genetic and molecular investigation in Arabidopsis
description	The chloroplast proteome contains thousands of different proteins that are encoded by the nuclear genome. These proteins are imported into the chloroplast via the action of the TOC translocase and associated downstream systems. Our recent work has revealed that the stability of the TOC complex is dynamically regulated by the ubiquitin-dependent chloroplast-associated protein degradation pathway. Here, we demonstrate that the TOC complex is also regulated by the small ubiquitin-like modifier (SUMO) system. Arabidopsis mutants representing almost the entire SUMO conjugation pathway can partially suppress the phenotype of ppi1, a pale-yellow mutant lacking the Toc33 protein. This suppression is linked to increased abundance of TOC proteins and improvements in chloroplast development. Moreover, data from molecular and biochemical experiments support a model in which the SUMO system directly regulates TOC protein stability. Thus, we have identified a regulatory link between the SUMO system and the chloroplast protein import machinery.
format	article
author	Samuel James Watson Na Li Yiting Ye Feijie Wu Qihua Ling R Paul Jarvis
author_facet	Samuel James Watson Na Li Yiting Ye Feijie Wu Qihua Ling R Paul Jarvis
author_sort	Samuel James Watson
title	Crosstalk between the chloroplast protein import and SUMO systems revealed through genetic and molecular investigation in Arabidopsis
title_short	Crosstalk between the chloroplast protein import and SUMO systems revealed through genetic and molecular investigation in Arabidopsis
title_full	Crosstalk between the chloroplast protein import and SUMO systems revealed through genetic and molecular investigation in Arabidopsis
title_fullStr	Crosstalk between the chloroplast protein import and SUMO systems revealed through genetic and molecular investigation in Arabidopsis
title_full_unstemmed	Crosstalk between the chloroplast protein import and SUMO systems revealed through genetic and molecular investigation in Arabidopsis
title_sort	crosstalk between the chloroplast protein import and sumo systems revealed through genetic and molecular investigation in arabidopsis
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/73a5eff73f614347bdc9a2b61ed299c5
work_keys_str_mv	AT samueljameswatson crosstalkbetweenthechloroplastproteinimportandsumosystemsrevealedthroughgeneticandmolecularinvestigationinarabidopsis AT nali crosstalkbetweenthechloroplastproteinimportandsumosystemsrevealedthroughgeneticandmolecularinvestigationinarabidopsis AT yitingye crosstalkbetweenthechloroplastproteinimportandsumosystemsrevealedthroughgeneticandmolecularinvestigationinarabidopsis AT feijiewu crosstalkbetweenthechloroplastproteinimportandsumosystemsrevealedthroughgeneticandmolecularinvestigationinarabidopsis AT qihualing crosstalkbetweenthechloroplastproteinimportandsumosystemsrevealedthroughgeneticandmolecularinvestigationinarabidopsis AT rpauljarvis crosstalkbetweenthechloroplastproteinimportandsumosystemsrevealedthroughgeneticandmolecularinvestigationinarabidopsis
_version_	1718416709003509760

Crosstalk between the chloroplast protein import and SUMO systems revealed through genetic and molecular investigation in Arabidopsis

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