Mycobacterium Avium subsp. paratuberculosis isolates induce in vitro granuloma formation and show successful survival phenotype, common anti-inflammatory and antiapoptotic responses within ovine macrophages regardless of genotype or host of origin.

The analysis of the early macrophage responses, including bacterial growth within macrophages, represents a powerful tool to characterize the virulence of clinical isolates of Mycobcaterium avium susbp. paratuberculosis (Map). The present study represents the first assessment of the intracellular be...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Naiara Abendaño, Lyudmila Tyukalova, Jesse F Barandika, Ana Balseiro, Iker A Sevilla, Joseba M Garrido, Ramon A Juste, Marta Alonso-Hearn
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2014
Materias:
R
Q
Acceso en línea:https://doaj.org/article/73af8eef7c09470a946114c710a27295
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:73af8eef7c09470a946114c710a27295
record_format dspace
spelling oai:doaj.org-article:73af8eef7c09470a946114c710a272952021-11-25T06:05:20ZMycobacterium Avium subsp. paratuberculosis isolates induce in vitro granuloma formation and show successful survival phenotype, common anti-inflammatory and antiapoptotic responses within ovine macrophages regardless of genotype or host of origin.1932-620310.1371/journal.pone.0104238https://doaj.org/article/73af8eef7c09470a946114c710a272952014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25111300/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The analysis of the early macrophage responses, including bacterial growth within macrophages, represents a powerful tool to characterize the virulence of clinical isolates of Mycobcaterium avium susbp. paratuberculosis (Map). The present study represents the first assessment of the intracellular behaviour in ovine monocyte-derived macrophages (MDMs) of Map isolates representing distinct genotypes (C, S and B), and isolated from cattle, sheep, goat, fallow deer, deer, and wild boar. Intracellular growth and survival of the selected isolates in ovine MDMs was assessed by quantification of CFUs inside of the host cells at 2 h p.i. (day 0) and 7 d p. i. using an automatic liquid culture system (Bactec MGIT 960). Variations in bacterial counts over 7 days from the baseline were small, in a range between 1.63 to 1.05-fold. After 7 d of infection, variations in the estimated log10 CFUs between all the tested isolates were not statistically significant. In addition, ovine MDMs exhibited enhanced anti-inflammatory, antiapoptotic and antidestructive responses when infected with two ovine isolates of distinct genotype (C and S) or with two C-type isolates from distinct hosts (cattle and sheep); which correlated with the successful survival of these isolates within ovine MDMs. A second objective was to study, based on an in vitro granuloma model, latter stages of the infection by investigating the capacity of two Map isolates from cattle and sheep to trigger formation of microgranulomas. Upon 10 d p.i., both Map isolates were able to induce the formation of granulomas comparable to the granulomas observed in clinical specimens with respect to the cellular components involved. In summary, our results demonstrated that Map isolates from cattle, sheep, goats, deer, fallow-deer and wild boar were able not only to initiate but also to establish a successful infection in ovine macrophages regardless of genotype.Naiara AbendañoLyudmila TyukalovaJesse F BarandikaAna BalseiroIker A SevillaJoseba M GarridoRamon A JusteMarta Alonso-HearnPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e104238 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Naiara Abendaño
Lyudmila Tyukalova
Jesse F Barandika
Ana Balseiro
Iker A Sevilla
Joseba M Garrido
Ramon A Juste
Marta Alonso-Hearn
Mycobacterium Avium subsp. paratuberculosis isolates induce in vitro granuloma formation and show successful survival phenotype, common anti-inflammatory and antiapoptotic responses within ovine macrophages regardless of genotype or host of origin.
description The analysis of the early macrophage responses, including bacterial growth within macrophages, represents a powerful tool to characterize the virulence of clinical isolates of Mycobcaterium avium susbp. paratuberculosis (Map). The present study represents the first assessment of the intracellular behaviour in ovine monocyte-derived macrophages (MDMs) of Map isolates representing distinct genotypes (C, S and B), and isolated from cattle, sheep, goat, fallow deer, deer, and wild boar. Intracellular growth and survival of the selected isolates in ovine MDMs was assessed by quantification of CFUs inside of the host cells at 2 h p.i. (day 0) and 7 d p. i. using an automatic liquid culture system (Bactec MGIT 960). Variations in bacterial counts over 7 days from the baseline were small, in a range between 1.63 to 1.05-fold. After 7 d of infection, variations in the estimated log10 CFUs between all the tested isolates were not statistically significant. In addition, ovine MDMs exhibited enhanced anti-inflammatory, antiapoptotic and antidestructive responses when infected with two ovine isolates of distinct genotype (C and S) or with two C-type isolates from distinct hosts (cattle and sheep); which correlated with the successful survival of these isolates within ovine MDMs. A second objective was to study, based on an in vitro granuloma model, latter stages of the infection by investigating the capacity of two Map isolates from cattle and sheep to trigger formation of microgranulomas. Upon 10 d p.i., both Map isolates were able to induce the formation of granulomas comparable to the granulomas observed in clinical specimens with respect to the cellular components involved. In summary, our results demonstrated that Map isolates from cattle, sheep, goats, deer, fallow-deer and wild boar were able not only to initiate but also to establish a successful infection in ovine macrophages regardless of genotype.
format article
author Naiara Abendaño
Lyudmila Tyukalova
Jesse F Barandika
Ana Balseiro
Iker A Sevilla
Joseba M Garrido
Ramon A Juste
Marta Alonso-Hearn
author_facet Naiara Abendaño
Lyudmila Tyukalova
Jesse F Barandika
Ana Balseiro
Iker A Sevilla
Joseba M Garrido
Ramon A Juste
Marta Alonso-Hearn
author_sort Naiara Abendaño
title Mycobacterium Avium subsp. paratuberculosis isolates induce in vitro granuloma formation and show successful survival phenotype, common anti-inflammatory and antiapoptotic responses within ovine macrophages regardless of genotype or host of origin.
title_short Mycobacterium Avium subsp. paratuberculosis isolates induce in vitro granuloma formation and show successful survival phenotype, common anti-inflammatory and antiapoptotic responses within ovine macrophages regardless of genotype or host of origin.
title_full Mycobacterium Avium subsp. paratuberculosis isolates induce in vitro granuloma formation and show successful survival phenotype, common anti-inflammatory and antiapoptotic responses within ovine macrophages regardless of genotype or host of origin.
title_fullStr Mycobacterium Avium subsp. paratuberculosis isolates induce in vitro granuloma formation and show successful survival phenotype, common anti-inflammatory and antiapoptotic responses within ovine macrophages regardless of genotype or host of origin.
title_full_unstemmed Mycobacterium Avium subsp. paratuberculosis isolates induce in vitro granuloma formation and show successful survival phenotype, common anti-inflammatory and antiapoptotic responses within ovine macrophages regardless of genotype or host of origin.
title_sort mycobacterium avium subsp. paratuberculosis isolates induce in vitro granuloma formation and show successful survival phenotype, common anti-inflammatory and antiapoptotic responses within ovine macrophages regardless of genotype or host of origin.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/73af8eef7c09470a946114c710a27295
work_keys_str_mv AT naiaraabendano mycobacteriumaviumsubspparatuberculosisisolatesinduceinvitrogranulomaformationandshowsuccessfulsurvivalphenotypecommonantiinflammatoryandantiapoptoticresponseswithinovinemacrophagesregardlessofgenotypeorhostoforigin
AT lyudmilatyukalova mycobacteriumaviumsubspparatuberculosisisolatesinduceinvitrogranulomaformationandshowsuccessfulsurvivalphenotypecommonantiinflammatoryandantiapoptoticresponseswithinovinemacrophagesregardlessofgenotypeorhostoforigin
AT jessefbarandika mycobacteriumaviumsubspparatuberculosisisolatesinduceinvitrogranulomaformationandshowsuccessfulsurvivalphenotypecommonantiinflammatoryandantiapoptoticresponseswithinovinemacrophagesregardlessofgenotypeorhostoforigin
AT anabalseiro mycobacteriumaviumsubspparatuberculosisisolatesinduceinvitrogranulomaformationandshowsuccessfulsurvivalphenotypecommonantiinflammatoryandantiapoptoticresponseswithinovinemacrophagesregardlessofgenotypeorhostoforigin
AT ikerasevilla mycobacteriumaviumsubspparatuberculosisisolatesinduceinvitrogranulomaformationandshowsuccessfulsurvivalphenotypecommonantiinflammatoryandantiapoptoticresponseswithinovinemacrophagesregardlessofgenotypeorhostoforigin
AT josebamgarrido mycobacteriumaviumsubspparatuberculosisisolatesinduceinvitrogranulomaformationandshowsuccessfulsurvivalphenotypecommonantiinflammatoryandantiapoptoticresponseswithinovinemacrophagesregardlessofgenotypeorhostoforigin
AT ramonajuste mycobacteriumaviumsubspparatuberculosisisolatesinduceinvitrogranulomaformationandshowsuccessfulsurvivalphenotypecommonantiinflammatoryandantiapoptoticresponseswithinovinemacrophagesregardlessofgenotypeorhostoforigin
AT martaalonsohearn mycobacteriumaviumsubspparatuberculosisisolatesinduceinvitrogranulomaformationandshowsuccessfulsurvivalphenotypecommonantiinflammatoryandantiapoptoticresponseswithinovinemacrophagesregardlessofgenotypeorhostoforigin
_version_ 1718414229076180992