Dpp signaling activity requires Pentagone to scale with tissue size in the growing Drosophila wing imaginal disc.

The wing of the fruit fly, Drosophila melanogaster, with its simple, two-dimensional structure, is a model organ well suited for a systems biology approach. The wing arises from an epithelial sac referred to as the wing imaginal disc, which undergoes a phase of massive growth and concomitant pattern...

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Autores principales:	Fisun Hamaratoglu, Aitana Morton de Lachapelle, George Pyrowolakis, Sven Bergmann, Markus Affolter
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/73b462f8f8d047eea40e0a4c944d13b6
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spelling	oai:doaj.org-article:73b462f8f8d047eea40e0a4c944d13b62021-11-18T05:36:55ZDpp signaling activity requires Pentagone to scale with tissue size in the growing Drosophila wing imaginal disc.1544-91731545-788510.1371/journal.pbio.1001182https://doaj.org/article/73b462f8f8d047eea40e0a4c944d13b62011-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22039350/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885The wing of the fruit fly, Drosophila melanogaster, with its simple, two-dimensional structure, is a model organ well suited for a systems biology approach. The wing arises from an epithelial sac referred to as the wing imaginal disc, which undergoes a phase of massive growth and concomitant patterning during larval stages. The Decapentaplegic (Dpp) morphogen plays a central role in wing formation with its ability to co-coordinately regulate patterning and growth. Here, we asked whether the Dpp signaling activity scales, i.e. expands proportionally, with the growing wing imaginal disc. Using new methods for spatial and temporal quantification of Dpp activity and its scaling properties, we found that the Dpp response scales with the size of the growing tissue. Notably, scaling is not perfect at all positions in the field and the scaling of target gene domains is ensured specifically where they define vein positions. We also found that the target gene domains are not defined at constant concentration thresholds of the downstream Dpp activity gradients P-Mad and Brinker. Most interestingly, Pentagone, an important secreted feedback regulator of the pathway, plays a central role in scaling and acts as an expander of the Dpp gradient during disc growth.Fisun HamaratogluAitana Morton de LachapelleGeorge PyrowolakisSven BergmannMarkus AffolterPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 9, Iss 10, p e1001182 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Fisun Hamaratoglu Aitana Morton de Lachapelle George Pyrowolakis Sven Bergmann Markus Affolter Dpp signaling activity requires Pentagone to scale with tissue size in the growing Drosophila wing imaginal disc.
description	The wing of the fruit fly, Drosophila melanogaster, with its simple, two-dimensional structure, is a model organ well suited for a systems biology approach. The wing arises from an epithelial sac referred to as the wing imaginal disc, which undergoes a phase of massive growth and concomitant patterning during larval stages. The Decapentaplegic (Dpp) morphogen plays a central role in wing formation with its ability to co-coordinately regulate patterning and growth. Here, we asked whether the Dpp signaling activity scales, i.e. expands proportionally, with the growing wing imaginal disc. Using new methods for spatial and temporal quantification of Dpp activity and its scaling properties, we found that the Dpp response scales with the size of the growing tissue. Notably, scaling is not perfect at all positions in the field and the scaling of target gene domains is ensured specifically where they define vein positions. We also found that the target gene domains are not defined at constant concentration thresholds of the downstream Dpp activity gradients P-Mad and Brinker. Most interestingly, Pentagone, an important secreted feedback regulator of the pathway, plays a central role in scaling and acts as an expander of the Dpp gradient during disc growth.
format	article
author	Fisun Hamaratoglu Aitana Morton de Lachapelle George Pyrowolakis Sven Bergmann Markus Affolter
author_facet	Fisun Hamaratoglu Aitana Morton de Lachapelle George Pyrowolakis Sven Bergmann Markus Affolter
author_sort	Fisun Hamaratoglu
title	Dpp signaling activity requires Pentagone to scale with tissue size in the growing Drosophila wing imaginal disc.
title_short	Dpp signaling activity requires Pentagone to scale with tissue size in the growing Drosophila wing imaginal disc.
title_full	Dpp signaling activity requires Pentagone to scale with tissue size in the growing Drosophila wing imaginal disc.
title_fullStr	Dpp signaling activity requires Pentagone to scale with tissue size in the growing Drosophila wing imaginal disc.
title_full_unstemmed	Dpp signaling activity requires Pentagone to scale with tissue size in the growing Drosophila wing imaginal disc.
title_sort	dpp signaling activity requires pentagone to scale with tissue size in the growing drosophila wing imaginal disc.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/73b462f8f8d047eea40e0a4c944d13b6
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Dpp signaling activity requires Pentagone to scale with tissue size in the growing Drosophila wing imaginal disc.

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