Analysis of ovarian transcriptomes reveals thousands of novel genes in the insect vector Rhodnius prolixus

Abstract Rhodnius prolixus is a Triatominae insect species and a primary vector of Chagas disease. The genome of R. prolixus has been recently sequenced and partially assembled, but few transcriptome analyses have been performed to date. In this study, we describe the stage-specific transcriptomes o...

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Autores principales:	Vitor Lima Coelho, Tarcísio Fontenele de Brito, Ingrid Alexandre de Abreu Brito, Maira Arruda Cardoso, Mateus Antonio Berni, Helena Maria Marcolla Araujo, Michael Sammeth, Attilio Pane
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/73bd35a73d5b415c9665f128b3d65802
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spelling	oai:doaj.org-article:73bd35a73d5b415c9665f128b3d658022021-12-02T11:50:40ZAnalysis of ovarian transcriptomes reveals thousands of novel genes in the insect vector Rhodnius prolixus10.1038/s41598-021-81387-12045-2322https://doaj.org/article/73bd35a73d5b415c9665f128b3d658022021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81387-1https://doaj.org/toc/2045-2322Abstract Rhodnius prolixus is a Triatominae insect species and a primary vector of Chagas disease. The genome of R. prolixus has been recently sequenced and partially assembled, but few transcriptome analyses have been performed to date. In this study, we describe the stage-specific transcriptomes obtained from previtellogenic stages of oogenesis and from mature eggs. By analyzing ~ 228 million paired-end RNA-Seq reads, we significantly improved the current genome annotations for 9206 genes. We provide extended 5′ and 3′ UTRs, complete Open Reading Frames, and alternative transcript variants. Strikingly, using a combination of genome-guided and de novo transcriptome assembly we found more than two thousand novel genes, thus increasing the number of genes in R. prolixus from 15,738 to 17,864. We used the improved transcriptome to investigate stage-specific gene expression profiles during R. prolixus oogenesis. Our data reveal that 11,127 genes are expressed in the early previtellogenic stage of oogenesis and their transcripts are deposited in the developing egg including key factors regulating germline development, genome integrity, and the maternal-zygotic transition. In addition, GO term analyses show that transcripts encoding components of the steroid hormone receptor pathway, cytoskeleton, and intracellular signaling are abundant in the mature eggs, where they likely control early embryonic development upon fertilization. Our results significantly improve the R. prolixus genome and transcriptome and provide novel insight into oogenesis and early embryogenesis in this medically relevant insect.Vitor Lima CoelhoTarcísio Fontenele de BritoIngrid Alexandre de Abreu BritoMaira Arruda CardosoMateus Antonio BerniHelena Maria Marcolla AraujoMichael SammethAttilio PaneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vitor Lima Coelho Tarcísio Fontenele de Brito Ingrid Alexandre de Abreu Brito Maira Arruda Cardoso Mateus Antonio Berni Helena Maria Marcolla Araujo Michael Sammeth Attilio Pane Analysis of ovarian transcriptomes reveals thousands of novel genes in the insect vector Rhodnius prolixus
description	Abstract Rhodnius prolixus is a Triatominae insect species and a primary vector of Chagas disease. The genome of R. prolixus has been recently sequenced and partially assembled, but few transcriptome analyses have been performed to date. In this study, we describe the stage-specific transcriptomes obtained from previtellogenic stages of oogenesis and from mature eggs. By analyzing ~ 228 million paired-end RNA-Seq reads, we significantly improved the current genome annotations for 9206 genes. We provide extended 5′ and 3′ UTRs, complete Open Reading Frames, and alternative transcript variants. Strikingly, using a combination of genome-guided and de novo transcriptome assembly we found more than two thousand novel genes, thus increasing the number of genes in R. prolixus from 15,738 to 17,864. We used the improved transcriptome to investigate stage-specific gene expression profiles during R. prolixus oogenesis. Our data reveal that 11,127 genes are expressed in the early previtellogenic stage of oogenesis and their transcripts are deposited in the developing egg including key factors regulating germline development, genome integrity, and the maternal-zygotic transition. In addition, GO term analyses show that transcripts encoding components of the steroid hormone receptor pathway, cytoskeleton, and intracellular signaling are abundant in the mature eggs, where they likely control early embryonic development upon fertilization. Our results significantly improve the R. prolixus genome and transcriptome and provide novel insight into oogenesis and early embryogenesis in this medically relevant insect.
format	article
author	Vitor Lima Coelho Tarcísio Fontenele de Brito Ingrid Alexandre de Abreu Brito Maira Arruda Cardoso Mateus Antonio Berni Helena Maria Marcolla Araujo Michael Sammeth Attilio Pane
author_facet	Vitor Lima Coelho Tarcísio Fontenele de Brito Ingrid Alexandre de Abreu Brito Maira Arruda Cardoso Mateus Antonio Berni Helena Maria Marcolla Araujo Michael Sammeth Attilio Pane
author_sort	Vitor Lima Coelho
title	Analysis of ovarian transcriptomes reveals thousands of novel genes in the insect vector Rhodnius prolixus
title_short	Analysis of ovarian transcriptomes reveals thousands of novel genes in the insect vector Rhodnius prolixus
title_full	Analysis of ovarian transcriptomes reveals thousands of novel genes in the insect vector Rhodnius prolixus
title_fullStr	Analysis of ovarian transcriptomes reveals thousands of novel genes in the insect vector Rhodnius prolixus
title_full_unstemmed	Analysis of ovarian transcriptomes reveals thousands of novel genes in the insect vector Rhodnius prolixus
title_sort	analysis of ovarian transcriptomes reveals thousands of novel genes in the insect vector rhodnius prolixus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/73bd35a73d5b415c9665f128b3d65802
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Analysis of ovarian transcriptomes reveals thousands of novel genes in the insect vector Rhodnius prolixus

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