Sublethal concentrations of spinosad synergize the pathogenicity of fungi to larvae of Ephestia kuehniella (Lepidoptera: Pyralidae)

We evaluated the efficacy of four entomopathogenic fungi (EPF) and their compatibility with the bioinsecticide spinosad for control of Ephestia kuehniella (Zeller) under laboratory conditions. Three EPF, including Beauveria bassiana (Balsamo-Criveili) Vuillemin isolates Z1 and Iran 1395C, Lecanicill...

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Autores principales:	Fariba SOHRABI, Fatemeh JAMALI, J.P. MICHAUD
Formato:	article
Lenguaje:	EN
Publicado:	Institute of Entomology, Biology Centre, Czech Academy of Science 2021
Materias:	lepidoptera pyralidae ephestia kuehniella entomopathogenic fungi beauveria bassiana purpureocillium lilacinum lecanicillium lecanii biopesticide spinosad stored products Zoology QL1-991
Acceso en línea:	https://doaj.org/article/73be846e578949afa1292dbb8b486536
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spelling	oai:doaj.org-article:73be846e578949afa1292dbb8b4865362021-11-05T15:23:15ZSublethal concentrations of spinosad synergize the pathogenicity of fungi to larvae of Ephestia kuehniella (Lepidoptera: Pyralidae)1210-57591802-882910.14411/eje.2021.015https://doaj.org/article/73be846e578949afa1292dbb8b4865362021-06-01T00:00:00Zhttps://www.eje.cz/artkey/eje-202101-0015_sublethal_concentrations_of_spinosad_synergize_the_pathogenicity_of_fungi_to_larvae_of_ephestia_kuehniella_lep.phphttps://doaj.org/toc/1210-5759https://doaj.org/toc/1802-8829We evaluated the efficacy of four entomopathogenic fungi (EPF) and their compatibility with the bioinsecticide spinosad for control of Ephestia kuehniella (Zeller) under laboratory conditions. Three EPF, including Beauveria bassiana (Balsamo-Criveili) Vuillemin isolates Z1 and Iran 1395C, Lecanicillium (= Verticillium) lecanii (Zimmerman) Zare & Gams, isolate Iran 229, and Purpureocillium (Paecilomyces) lilacinum (Thom) Luangs-ard, Hywel-Jones & Samson, isolate Iran 1026 were tested against third and fifth larval instars of Ephestia kuehniella using a filter paper bioassay. Mortality caused by the EPF ranged from 63.3-72.5% for third instars and 50-65.5% for fifth instars, with LT50 ranging from 8.4-10.5 d and 10.1-12.9 d, respectively. The effect of spinosad at LC10 (= 26.2 ppm) on EPF spore germination was evaluated and found to be negligible, ranging from 0% for B. bassiana Z1 to 5.7% for P. lilacinum. The LC50 values for spinosad against third and fifth instar E. kuehniella larvae were 452.5 and 1446 ppm, respectively. Subsequently, spinosad at LC10 was applied to third instar E. kuehniella larvae 24 h before application of the EPFs at LC50. The addition of spinosad to applications of L. lecanii and B. bassiana Z1 and Iran1395C isolates synergized their pathogenicity to E. kuehniella larvae, whereas the effect was merely additive for P. lilacinum. Our results suggest that these EPF isolates can be used effectively in combination with spinosad for management of E. kuehniella in stored products.Fariba SOHRABIFatemeh JAMALIJ.P. MICHAUDInstitute of Entomology, Biology Centre, Czech Academy of Sciencearticlelepidopterapyralidaeephestia kuehniellaentomopathogenic fungibeauveria bassianapurpureocillium lilacinumlecanicillium lecaniibiopesticidespinosadstored productsZoologyQL1-991ENEuropean Journal of Entomology, Vol 118, Iss 1, Pp 142-147 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lepidoptera pyralidae ephestia kuehniella entomopathogenic fungi beauveria bassiana purpureocillium lilacinum lecanicillium lecanii biopesticide spinosad stored products Zoology QL1-991
spellingShingle	lepidoptera pyralidae ephestia kuehniella entomopathogenic fungi beauveria bassiana purpureocillium lilacinum lecanicillium lecanii biopesticide spinosad stored products Zoology QL1-991 Fariba SOHRABI Fatemeh JAMALI J.P. MICHAUD Sublethal concentrations of spinosad synergize the pathogenicity of fungi to larvae of Ephestia kuehniella (Lepidoptera: Pyralidae)
description	We evaluated the efficacy of four entomopathogenic fungi (EPF) and their compatibility with the bioinsecticide spinosad for control of Ephestia kuehniella (Zeller) under laboratory conditions. Three EPF, including Beauveria bassiana (Balsamo-Criveili) Vuillemin isolates Z1 and Iran 1395C, Lecanicillium (= Verticillium) lecanii (Zimmerman) Zare & Gams, isolate Iran 229, and Purpureocillium (Paecilomyces) lilacinum (Thom) Luangs-ard, Hywel-Jones & Samson, isolate Iran 1026 were tested against third and fifth larval instars of Ephestia kuehniella using a filter paper bioassay. Mortality caused by the EPF ranged from 63.3-72.5% for third instars and 50-65.5% for fifth instars, with LT50 ranging from 8.4-10.5 d and 10.1-12.9 d, respectively. The effect of spinosad at LC10 (= 26.2 ppm) on EPF spore germination was evaluated and found to be negligible, ranging from 0% for B. bassiana Z1 to 5.7% for P. lilacinum. The LC50 values for spinosad against third and fifth instar E. kuehniella larvae were 452.5 and 1446 ppm, respectively. Subsequently, spinosad at LC10 was applied to third instar E. kuehniella larvae 24 h before application of the EPFs at LC50. The addition of spinosad to applications of L. lecanii and B. bassiana Z1 and Iran1395C isolates synergized their pathogenicity to E. kuehniella larvae, whereas the effect was merely additive for P. lilacinum. Our results suggest that these EPF isolates can be used effectively in combination with spinosad for management of E. kuehniella in stored products.
format	article
author	Fariba SOHRABI Fatemeh JAMALI J.P. MICHAUD
author_facet	Fariba SOHRABI Fatemeh JAMALI J.P. MICHAUD
author_sort	Fariba SOHRABI
title	Sublethal concentrations of spinosad synergize the pathogenicity of fungi to larvae of Ephestia kuehniella (Lepidoptera: Pyralidae)
title_short	Sublethal concentrations of spinosad synergize the pathogenicity of fungi to larvae of Ephestia kuehniella (Lepidoptera: Pyralidae)
title_full	Sublethal concentrations of spinosad synergize the pathogenicity of fungi to larvae of Ephestia kuehniella (Lepidoptera: Pyralidae)
title_fullStr	Sublethal concentrations of spinosad synergize the pathogenicity of fungi to larvae of Ephestia kuehniella (Lepidoptera: Pyralidae)
title_full_unstemmed	Sublethal concentrations of spinosad synergize the pathogenicity of fungi to larvae of Ephestia kuehniella (Lepidoptera: Pyralidae)
title_sort	sublethal concentrations of spinosad synergize the pathogenicity of fungi to larvae of ephestia kuehniella (lepidoptera: pyralidae)
publisher	Institute of Entomology, Biology Centre, Czech Academy of Science
publishDate	2021
url	https://doaj.org/article/73be846e578949afa1292dbb8b486536
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Sublethal concentrations of spinosad synergize the pathogenicity of fungi to larvae of Ephestia kuehniella (Lepidoptera: Pyralidae)

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