Topical Administration of 15-Deoxy-Δ12,14-Prostaglandin J2 Using a Nonionic Cream: Effect on UVB-Induced Skin Oxidative, Inflammatory, and Histopathological Modifications in Mice

UVB radiation is certainly one of the most important environmental threats to which we are subjected to. This fact highlights the crucial protective role of the skin. However, the skin itself may not be capable of protecting against UVB depending on irradiation intensity and time of exposition. Sun...

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Autores principales:	Clovis M. Kumagai, Renata M. Martinez, Barbara B. Colombo, Priscila Saito, Ingrid C. Pinto, Camilla C. A. Rodrigues, David L. Vale, Ricardo L. N. Matos, Ana P. F. R. L. Bracarense, Marcela M. Baracat, Sandra R. Georgetti, Juliana T. Clemente-Napimoga, Marcelo H. Napimoga, Victor Fattori, Waldiceu A. Verri, Rúbia Casagrande
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Pathology RB1-214
Acceso en línea:	https://doaj.org/article/73cb784d0d204695aac4d98f957cd1bc
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spelling	oai:doaj.org-article:73cb784d0d204695aac4d98f957cd1bc2021-11-15T01:19:50ZTopical Administration of 15-Deoxy-Δ12,14-Prostaglandin J2 Using a Nonionic Cream: Effect on UVB-Induced Skin Oxidative, Inflammatory, and Histopathological Modifications in Mice1466-186110.1155/2021/9330596https://doaj.org/article/73cb784d0d204695aac4d98f957cd1bc2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/9330596https://doaj.org/toc/1466-1861UVB radiation is certainly one of the most important environmental threats to which we are subjected to. This fact highlights the crucial protective role of the skin. However, the skin itself may not be capable of protecting against UVB depending on irradiation intensity and time of exposition. Sun blockers are used to protect our skin, but they fail to fully protect it against oxidative and inflammatory injuries initiated by UVB. To solve this issue, topical administration of active molecules is an option. 15-Deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) is an arachidonic acid-derived lipid with proresolution and anti-inflammatory actions. However, as far as we are aware, there is no evidence of its therapeutic use in a topical formulation to treat the deleterious events initiated by UVB, which was the aim of the present study. We used a nonionic cream to vehiculate 15d-PGJ2 (30, 90, and 300 ng/mouse) (TFcPGJ2) in the skin of hairless mice. UVB increased skin edema, myeloperoxidase activity, metalloproteinase-9 activity, lipid peroxidation, superoxide anion production, gp91phox and COX-2 mRNA expression, cytokine production, sunburn and mast cells, thickening of the epidermis, and collagen degradation. UVB also diminished skin ability to reduce iron and scavenge free radicals, reduced glutathione (GSH), sulfhydryl proteins, and catalase activity. TFcPGJ2 inhibited all these pathological alterations in the skin caused by UVB. No activity was observed with the unloaded topical formulation. The protective outcome of TFcPGJ2 indicates it is a promising therapeutic approach against cutaneous inflammatory and oxidative pathological alterations.Clovis M. KumagaiRenata M. MartinezBarbara B. ColomboPriscila SaitoIngrid C. PintoCamilla C. A. RodriguesDavid L. ValeRicardo L. N. MatosAna P. F. R. L. BracarenseMarcela M. BaracatSandra R. GeorgettiJuliana T. Clemente-NapimogaMarcelo H. NapimogaVictor FattoriWaldiceu A. VerriRúbia CasagrandeHindawi LimitedarticlePathologyRB1-214ENMediators of Inflammation, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pathology RB1-214
spellingShingle	Pathology RB1-214 Clovis M. Kumagai Renata M. Martinez Barbara B. Colombo Priscila Saito Ingrid C. Pinto Camilla C. A. Rodrigues David L. Vale Ricardo L. N. Matos Ana P. F. R. L. Bracarense Marcela M. Baracat Sandra R. Georgetti Juliana T. Clemente-Napimoga Marcelo H. Napimoga Victor Fattori Waldiceu A. Verri Rúbia Casagrande Topical Administration of 15-Deoxy-Δ12,14-Prostaglandin J2 Using a Nonionic Cream: Effect on UVB-Induced Skin Oxidative, Inflammatory, and Histopathological Modifications in Mice
description	UVB radiation is certainly one of the most important environmental threats to which we are subjected to. This fact highlights the crucial protective role of the skin. However, the skin itself may not be capable of protecting against UVB depending on irradiation intensity and time of exposition. Sun blockers are used to protect our skin, but they fail to fully protect it against oxidative and inflammatory injuries initiated by UVB. To solve this issue, topical administration of active molecules is an option. 15-Deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) is an arachidonic acid-derived lipid with proresolution and anti-inflammatory actions. However, as far as we are aware, there is no evidence of its therapeutic use in a topical formulation to treat the deleterious events initiated by UVB, which was the aim of the present study. We used a nonionic cream to vehiculate 15d-PGJ2 (30, 90, and 300 ng/mouse) (TFcPGJ2) in the skin of hairless mice. UVB increased skin edema, myeloperoxidase activity, metalloproteinase-9 activity, lipid peroxidation, superoxide anion production, gp91phox and COX-2 mRNA expression, cytokine production, sunburn and mast cells, thickening of the epidermis, and collagen degradation. UVB also diminished skin ability to reduce iron and scavenge free radicals, reduced glutathione (GSH), sulfhydryl proteins, and catalase activity. TFcPGJ2 inhibited all these pathological alterations in the skin caused by UVB. No activity was observed with the unloaded topical formulation. The protective outcome of TFcPGJ2 indicates it is a promising therapeutic approach against cutaneous inflammatory and oxidative pathological alterations.
format	article
author	Clovis M. Kumagai Renata M. Martinez Barbara B. Colombo Priscila Saito Ingrid C. Pinto Camilla C. A. Rodrigues David L. Vale Ricardo L. N. Matos Ana P. F. R. L. Bracarense Marcela M. Baracat Sandra R. Georgetti Juliana T. Clemente-Napimoga Marcelo H. Napimoga Victor Fattori Waldiceu A. Verri Rúbia Casagrande
author_facet	Clovis M. Kumagai Renata M. Martinez Barbara B. Colombo Priscila Saito Ingrid C. Pinto Camilla C. A. Rodrigues David L. Vale Ricardo L. N. Matos Ana P. F. R. L. Bracarense Marcela M. Baracat Sandra R. Georgetti Juliana T. Clemente-Napimoga Marcelo H. Napimoga Victor Fattori Waldiceu A. Verri Rúbia Casagrande
author_sort	Clovis M. Kumagai
title	Topical Administration of 15-Deoxy-Δ12,14-Prostaglandin J2 Using a Nonionic Cream: Effect on UVB-Induced Skin Oxidative, Inflammatory, and Histopathological Modifications in Mice
title_short	Topical Administration of 15-Deoxy-Δ12,14-Prostaglandin J2 Using a Nonionic Cream: Effect on UVB-Induced Skin Oxidative, Inflammatory, and Histopathological Modifications in Mice
title_full	Topical Administration of 15-Deoxy-Δ12,14-Prostaglandin J2 Using a Nonionic Cream: Effect on UVB-Induced Skin Oxidative, Inflammatory, and Histopathological Modifications in Mice
title_fullStr	Topical Administration of 15-Deoxy-Δ12,14-Prostaglandin J2 Using a Nonionic Cream: Effect on UVB-Induced Skin Oxidative, Inflammatory, and Histopathological Modifications in Mice
title_full_unstemmed	Topical Administration of 15-Deoxy-Δ12,14-Prostaglandin J2 Using a Nonionic Cream: Effect on UVB-Induced Skin Oxidative, Inflammatory, and Histopathological Modifications in Mice
title_sort	topical administration of 15-deoxy-δ12,14-prostaglandin j2 using a nonionic cream: effect on uvb-induced skin oxidative, inflammatory, and histopathological modifications in mice
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/73cb784d0d204695aac4d98f957cd1bc
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Topical Administration of 15-Deoxy-Δ12,14-Prostaglandin J2 Using a Nonionic Cream: Effect on UVB-Induced Skin Oxidative, Inflammatory, and Histopathological Modifications in Mice

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