Enhanced growth and differentiation of myoblast cells grown on E-jet 3D printed platforms

Haoxiang Chen,* Juchang Zhong,* Jian Wang, Ruiying Huang, Xiaoyin Qiao, Honghui Wang, Zhikai Tan College of Biology, Hunan University, Changsha, Hunan 410082, China *These authors contributed equally to this work Background: Skeletal muscle tissue engineering often involves the prefabrication of...

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Autores principales:	Chen H, Zhong J, Wang J, Huang R, Qiao X, Wang H, Tan Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Myoblast Myogenic differentiation 3D printing 3D culture Skeletal muscle regeneration Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/73d0a9c65b6f403781058e83ff15c060
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spelling	oai:doaj.org-article:73d0a9c65b6f403781058e83ff15c0602021-12-02T03:55:15ZEnhanced growth and differentiation of myoblast cells grown on E-jet 3D printed platforms1178-2013https://doaj.org/article/73d0a9c65b6f403781058e83ff15c0602019-02-01T00:00:00Zhttps://www.dovepress.com/enhanced-growth-and-differentiation-of-myoblast-cells-grown-on-e-jet-3-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Haoxiang Chen,* Juchang Zhong,* Jian Wang, Ruiying Huang, Xiaoyin Qiao, Honghui Wang, Zhikai Tan College of Biology, Hunan University, Changsha, Hunan 410082, China *These authors contributed equally to this work Background: Skeletal muscle tissue engineering often involves the prefabrication of muscle tissues in vitro by differentiation and maturation of muscle precursor cells on a platform which provides an environment that facilitates the myogenic differentiation of the seeded cells.Methods: Poly lactic-co-glycolic acid (PLGA) 3D printed scaffolds, which simulate the highly complex structure of extracellular matrix (ECM), were fabricated by E-jet 3D printing in this study. The scaffolds were used as platforms, providing environment that aids in growth, differentiation and other properties of C2C12 myoblast cells.Results: The C2C12 myoblast cells grown on the PLGA 3D printed platforms had enhanced cell adhesion and proliferation. Moreover, the platforms were able to induce myogenic differentiation of the myoblast cells by promoting the formation of myotubes and up-regulating the expressions of myogenic genes (MyHC and MyOG).Conclusion: The fabricated 3D printed platforms have excellent biocompatibility, thereby can potentially be used as functional cell culture platforms in skeletal tissue engineering and regeneration. Keywords: myoblast, myogenic differentiation, 3D printing, 3D cell culture, skeletal muscle regenerationChen HZhong JWang JHuang RQiao XWang HTan ZDove Medical PressarticleMyoblastMyogenic differentiation3D printing3D cultureSkeletal muscle regenerationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 937-950 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Myoblast Myogenic differentiation 3D printing 3D culture Skeletal muscle regeneration Medicine (General) R5-920
spellingShingle	Myoblast Myogenic differentiation 3D printing 3D culture Skeletal muscle regeneration Medicine (General) R5-920 Chen H Zhong J Wang J Huang R Qiao X Wang H Tan Z Enhanced growth and differentiation of myoblast cells grown on E-jet 3D printed platforms
description	Haoxiang Chen,* Juchang Zhong,* Jian Wang, Ruiying Huang, Xiaoyin Qiao, Honghui Wang, Zhikai Tan College of Biology, Hunan University, Changsha, Hunan 410082, China *These authors contributed equally to this work Background: Skeletal muscle tissue engineering often involves the prefabrication of muscle tissues in vitro by differentiation and maturation of muscle precursor cells on a platform which provides an environment that facilitates the myogenic differentiation of the seeded cells.Methods: Poly lactic-co-glycolic acid (PLGA) 3D printed scaffolds, which simulate the highly complex structure of extracellular matrix (ECM), were fabricated by E-jet 3D printing in this study. The scaffolds were used as platforms, providing environment that aids in growth, differentiation and other properties of C2C12 myoblast cells.Results: The C2C12 myoblast cells grown on the PLGA 3D printed platforms had enhanced cell adhesion and proliferation. Moreover, the platforms were able to induce myogenic differentiation of the myoblast cells by promoting the formation of myotubes and up-regulating the expressions of myogenic genes (MyHC and MyOG).Conclusion: The fabricated 3D printed platforms have excellent biocompatibility, thereby can potentially be used as functional cell culture platforms in skeletal tissue engineering and regeneration. Keywords: myoblast, myogenic differentiation, 3D printing, 3D cell culture, skeletal muscle regeneration
format	article
author	Chen H Zhong J Wang J Huang R Qiao X Wang H Tan Z
author_facet	Chen H Zhong J Wang J Huang R Qiao X Wang H Tan Z
author_sort	Chen H
title	Enhanced growth and differentiation of myoblast cells grown on E-jet 3D printed platforms
title_short	Enhanced growth and differentiation of myoblast cells grown on E-jet 3D printed platforms
title_full	Enhanced growth and differentiation of myoblast cells grown on E-jet 3D printed platforms
title_fullStr	Enhanced growth and differentiation of myoblast cells grown on E-jet 3D printed platforms
title_full_unstemmed	Enhanced growth and differentiation of myoblast cells grown on E-jet 3D printed platforms
title_sort	enhanced growth and differentiation of myoblast cells grown on e-jet 3d printed platforms
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/73d0a9c65b6f403781058e83ff15c060
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Enhanced growth and differentiation of myoblast cells grown on E-jet 3D printed platforms

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