Taxon-Driven Functional Shifts Associated with Storm Flow in an Urban Stream Microbial Community

ABSTRACT Urban streams are susceptible to stormwater and sewage inputs that can impact their ecological health and water quality. Microbial communities in streams play important functional roles, and their composition and metabolic potential can help assess ecological state and water quality. Althou...

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Autores principales:	Adit Chaudhary, Imrose Kauser, Anirban Ray, Rachel Poretsky
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	metagenomics microbial communities storm flow urban streams Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/73d250c59ed64c389a5a8b8c9b74c374
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spelling	oai:doaj.org-article:73d250c59ed64c389a5a8b8c9b74c3742021-11-15T15:25:50ZTaxon-Driven Functional Shifts Associated with Storm Flow in an Urban Stream Microbial Community10.1128/mSphere.00194-182379-5042https://doaj.org/article/73d250c59ed64c389a5a8b8c9b74c3742018-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00194-18https://doaj.org/toc/2379-5042ABSTRACT Urban streams are susceptible to stormwater and sewage inputs that can impact their ecological health and water quality. Microbial communities in streams play important functional roles, and their composition and metabolic potential can help assess ecological state and water quality. Although these environments are highly heterogenous, little is known about the influence of isolated perturbations, such as those resulting from rain events on urban stream microbiota. Here, we examined the microbial community composition and diversity in an urban stream during dry and wet weather conditions with both 16S rRNA gene sequencing across multiple years and shotgun metagenomics to more deeply analyze a single storm flow event. Metagenomics was used to assess population-level dynamics as well as shifts in the microbial community taxonomic profile and functional potential before and after a substantial rainfall. The results demonstrated general trends present in the stream under storm flow versus base flow conditions and also highlighted the influence of increased effluent flow following rain in shifting the stream microbial community from abundant freshwater taxa to those more associated with urban/anthropogenic settings. Shifts in the taxonomic composition were also linked to changes in functional gene content, particularly for transmembrane transport and organic substance biosynthesis. We also observed an increase in relative abundance of genes encoding degradation of organic pollutants and antibiotic resistance after rain. Overall, this study highlighted some differences in the microbial community of an urban stream under storm flow conditions and showed the impact of a storm flow event on the microbiome from an environmental and public health perspective. IMPORTANCE Urban streams in various parts of the world are facing increased anthropogenic pressure on their water quality, and storm flow events represent one such source of complex physical, chemical, and biological perturbations. Microorganisms are important components of these streams from both ecological and public health perspectives. Analysis of the effect of perturbations on the stream microbial community can help improve current knowledge on the impact such chronic disturbances can have on these water resources. This study examines microbial community dynamics during rain-induced storm flow conditions in an urban stream of the Chicago Area Waterway System. Additionally, using shotgun metagenomics we identified significant shifts in the microbial community composition and functional gene content following a high-rainfall event, with potential environment and public health implications. Previous work in this area has focused on specific genes/organisms or has not assessed immediate storm flow impact.Adit ChaudharyImrose KauserAnirban RayRachel PoretskyAmerican Society for Microbiologyarticlemetagenomicsmicrobial communitiesstorm flowurban streamsMicrobiologyQR1-502ENmSphere, Vol 3, Iss 4 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	metagenomics microbial communities storm flow urban streams Microbiology QR1-502
spellingShingle	metagenomics microbial communities storm flow urban streams Microbiology QR1-502 Adit Chaudhary Imrose Kauser Anirban Ray Rachel Poretsky Taxon-Driven Functional Shifts Associated with Storm Flow in an Urban Stream Microbial Community
description	ABSTRACT Urban streams are susceptible to stormwater and sewage inputs that can impact their ecological health and water quality. Microbial communities in streams play important functional roles, and their composition and metabolic potential can help assess ecological state and water quality. Although these environments are highly heterogenous, little is known about the influence of isolated perturbations, such as those resulting from rain events on urban stream microbiota. Here, we examined the microbial community composition and diversity in an urban stream during dry and wet weather conditions with both 16S rRNA gene sequencing across multiple years and shotgun metagenomics to more deeply analyze a single storm flow event. Metagenomics was used to assess population-level dynamics as well as shifts in the microbial community taxonomic profile and functional potential before and after a substantial rainfall. The results demonstrated general trends present in the stream under storm flow versus base flow conditions and also highlighted the influence of increased effluent flow following rain in shifting the stream microbial community from abundant freshwater taxa to those more associated with urban/anthropogenic settings. Shifts in the taxonomic composition were also linked to changes in functional gene content, particularly for transmembrane transport and organic substance biosynthesis. We also observed an increase in relative abundance of genes encoding degradation of organic pollutants and antibiotic resistance after rain. Overall, this study highlighted some differences in the microbial community of an urban stream under storm flow conditions and showed the impact of a storm flow event on the microbiome from an environmental and public health perspective. IMPORTANCE Urban streams in various parts of the world are facing increased anthropogenic pressure on their water quality, and storm flow events represent one such source of complex physical, chemical, and biological perturbations. Microorganisms are important components of these streams from both ecological and public health perspectives. Analysis of the effect of perturbations on the stream microbial community can help improve current knowledge on the impact such chronic disturbances can have on these water resources. This study examines microbial community dynamics during rain-induced storm flow conditions in an urban stream of the Chicago Area Waterway System. Additionally, using shotgun metagenomics we identified significant shifts in the microbial community composition and functional gene content following a high-rainfall event, with potential environment and public health implications. Previous work in this area has focused on specific genes/organisms or has not assessed immediate storm flow impact.
format	article
author	Adit Chaudhary Imrose Kauser Anirban Ray Rachel Poretsky
author_facet	Adit Chaudhary Imrose Kauser Anirban Ray Rachel Poretsky
author_sort	Adit Chaudhary
title	Taxon-Driven Functional Shifts Associated with Storm Flow in an Urban Stream Microbial Community
title_short	Taxon-Driven Functional Shifts Associated with Storm Flow in an Urban Stream Microbial Community
title_full	Taxon-Driven Functional Shifts Associated with Storm Flow in an Urban Stream Microbial Community
title_fullStr	Taxon-Driven Functional Shifts Associated with Storm Flow in an Urban Stream Microbial Community
title_full_unstemmed	Taxon-Driven Functional Shifts Associated with Storm Flow in an Urban Stream Microbial Community
title_sort	taxon-driven functional shifts associated with storm flow in an urban stream microbial community
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/73d250c59ed64c389a5a8b8c9b74c374
work_keys_str_mv	AT aditchaudhary taxondrivenfunctionalshiftsassociatedwithstormflowinanurbanstreammicrobialcommunity AT imrosekauser taxondrivenfunctionalshiftsassociatedwithstormflowinanurbanstreammicrobialcommunity AT anirbanray taxondrivenfunctionalshiftsassociatedwithstormflowinanurbanstreammicrobialcommunity AT rachelporetsky taxondrivenfunctionalshiftsassociatedwithstormflowinanurbanstreammicrobialcommunity
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Taxon-Driven Functional Shifts Associated with Storm Flow in an Urban Stream Microbial Community

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