Expression of pyruvate carboxylase mRNA variants in liver of dairy cattle at calving.

<h4>Background</h4>Bovine liver expresses six pyruvate carboxylase (PC) transcript variants, bPC5'A, bPC5'B, bPC5'C, bPC5'D, bPC5'E, and bPC5'F, which only differ at the 5' untranslated region (UTR) and contain a common coding region. The objective of t...

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Autores principales:	Cansu Agca, Shawn S Donkin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2007
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/73d714721ad042a19aa846b720b7e569
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spelling	oai:doaj.org-article:73d714721ad042a19aa846b720b7e5692021-11-25T06:13:44ZExpression of pyruvate carboxylase mRNA variants in liver of dairy cattle at calving.1932-620310.1371/journal.pone.0001270https://doaj.org/article/73d714721ad042a19aa846b720b7e5692007-12-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0001270https://doaj.org/toc/1932-6203<h4>Background</h4>Bovine liver expresses six pyruvate carboxylase (PC) transcript variants, bPC5'A, bPC5'B, bPC5'C, bPC5'D, bPC5'E, and bPC5'F, which only differ at the 5' untranslated region (UTR) and contain a common coding region. The objective of this experiment was to determine the profile and abundance of PC transcripts in bovine liver at calving.<h4>Methodology/principal findings</h4>A ribonuclease protection assay (RPA) protocol was developed to simplify analysis of these variants and investigate the changes in abundance of each 5' UTR transcript relative to total PC mRNA. Liver biopsy samples collected from seven cows on +1 d relative to calving were analyzed by RPA to determine the profile in PC 5' UTR variants. Results show that all six bovine PC 5' UTR variants are detected at calving. Data indicate that bovine PC 5' UTR variant A is the most abundant, variants C and E are least abundant and expression of variants B, D and F is intermediate at calving.<h4>Conclusions</h4>This manuscript describes a simplified RPA method that quantifies the abundance of six PC transcripts by using two riboprobes. The lack of uniformity in the pattern of PC 5' UTR variants at calving suggests an additional complexity for control of bovine PC mRNA expression at calving that may be the result of transcriptional controls, variation in mRNA processing, or a combination of these processes.Cansu AgcaShawn S DonkinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 2, Iss 12, p e1270 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cansu Agca Shawn S Donkin Expression of pyruvate carboxylase mRNA variants in liver of dairy cattle at calving.
description	<h4>Background</h4>Bovine liver expresses six pyruvate carboxylase (PC) transcript variants, bPC5'A, bPC5'B, bPC5'C, bPC5'D, bPC5'E, and bPC5'F, which only differ at the 5' untranslated region (UTR) and contain a common coding region. The objective of this experiment was to determine the profile and abundance of PC transcripts in bovine liver at calving.<h4>Methodology/principal findings</h4>A ribonuclease protection assay (RPA) protocol was developed to simplify analysis of these variants and investigate the changes in abundance of each 5' UTR transcript relative to total PC mRNA. Liver biopsy samples collected from seven cows on +1 d relative to calving were analyzed by RPA to determine the profile in PC 5' UTR variants. Results show that all six bovine PC 5' UTR variants are detected at calving. Data indicate that bovine PC 5' UTR variant A is the most abundant, variants C and E are least abundant and expression of variants B, D and F is intermediate at calving.<h4>Conclusions</h4>This manuscript describes a simplified RPA method that quantifies the abundance of six PC transcripts by using two riboprobes. The lack of uniformity in the pattern of PC 5' UTR variants at calving suggests an additional complexity for control of bovine PC mRNA expression at calving that may be the result of transcriptional controls, variation in mRNA processing, or a combination of these processes.
format	article
author	Cansu Agca Shawn S Donkin
author_facet	Cansu Agca Shawn S Donkin
author_sort	Cansu Agca
title	Expression of pyruvate carboxylase mRNA variants in liver of dairy cattle at calving.
title_short	Expression of pyruvate carboxylase mRNA variants in liver of dairy cattle at calving.
title_full	Expression of pyruvate carboxylase mRNA variants in liver of dairy cattle at calving.
title_fullStr	Expression of pyruvate carboxylase mRNA variants in liver of dairy cattle at calving.
title_full_unstemmed	Expression of pyruvate carboxylase mRNA variants in liver of dairy cattle at calving.
title_sort	expression of pyruvate carboxylase mrna variants in liver of dairy cattle at calving.
publisher	Public Library of Science (PLoS)
publishDate	2007
url	https://doaj.org/article/73d714721ad042a19aa846b720b7e569
work_keys_str_mv	AT cansuagca expressionofpyruvatecarboxylasemrnavariantsinliverofdairycattleatcalving AT shawnsdonkin expressionofpyruvatecarboxylasemrnavariantsinliverofdairycattleatcalving
_version_	1718413990915211264

Expression of pyruvate carboxylase mRNA variants in liver of dairy cattle at calving.

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