Molecular Mapping and Analysis of an Excellent Quantitative Trait Loci Conferring Adult-Plant Resistance to Stripe Rust in Chinese Wheat Landrace Gaoxianguangtoumai

The Chinese wheat landrace “Gaoxianguangtoumai” (GX) has exhibited a high level of adult-plant resistance (APR) to stripe rust in the field for more than a decade. To reveal the genetic background for APR to stripe rust in GX, a set of 249 F6:8 (F6, F7, and F8) recombinant inbred lines (RILs) was de...

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Autores principales:	Yuqi Wang, Fengying Liang, Fangnian Guan, Fangjie Yao, Li Long, Xuyang Zhao, Luyao Duan, Yu Wu, Hao Li, Wei Li, Qiantao Jiang, Yuming Wei, Jian Ma, Pengfei Qi, Mei Deng, Youliang Zheng, Houyang Kang, Yunfeng Jiang, Guoyue Chen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	adult-plant resistance QTL stripe rust Chinese wheat landrace genetic mapping heterogeneous inbred family Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/73da255517314ac8a0b4f2056f181005
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spelling	oai:doaj.org-article:73da255517314ac8a0b4f2056f1810052021-11-10T16:29:58ZMolecular Mapping and Analysis of an Excellent Quantitative Trait Loci Conferring Adult-Plant Resistance to Stripe Rust in Chinese Wheat Landrace Gaoxianguangtoumai1664-462X10.3389/fpls.2021.756557https://doaj.org/article/73da255517314ac8a0b4f2056f1810052021-10-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.756557/fullhttps://doaj.org/toc/1664-462XThe Chinese wheat landrace “Gaoxianguangtoumai” (GX) has exhibited a high level of adult-plant resistance (APR) to stripe rust in the field for more than a decade. To reveal the genetic background for APR to stripe rust in GX, a set of 249 F6:8 (F6, F7, and F8) recombinant inbred lines (RILs) was developed from a cross between GX and the susceptible cultivar “Taichung 29.” The parents and RILs were evaluated for disease severity at the adult-plant stage in the field by artificial inoculation with the currently predominant Chinese Puccinia striiformis f. sp. tritici races during three cropping seasons and genotyped using the Wheat 55K single-nucleotide polymorphism (SNP) array to construct a genetic map with 1,871 SNP markers finally. Two stable APR quantitative trait loci (QTL), QYr.GX-2AS and QYr.GX-7DS in GX, were detected on chromosomes 2AS and 7DS, which explained 15.5–27.0% and 11.5–13.5% of the total phenotypic variation, respectively. Compared with published Yr genes and QTL, QYr.GX-7DS and Yr18 may be the same, whereas QYr.GX-2AS is likely to be novel. Haplotype analysis revealed that QYr.GX-2AS is likely to be rare which presents in 5.3% of the 325 surveyed Chinese wheat landraces. By analyzing a heterogeneous inbred family (HIF) population from a residual heterozygous plant in an F8 generation of RIL, QYr.GX-2AS was further flanked by KP2A_36.85 and KP2A_38.22 with a physical distance of about 1.37Mb and co-segregated with the KP2A_37.09. Furthermore, three tightly linked Kompetitive allele-specific PCR (KASP) markers were highly polymorphic among 109 Chinese wheat cultivars. The results of this study can be used in wheat breeding for improving resistance to stripe rust.Yuqi WangYuqi WangFengying LiangFangnian GuanFangjie YaoLi LongXuyang ZhaoLuyao DuanYu WuHao LiWei LiQiantao JiangQiantao JiangYuming WeiYuming WeiJian MaPengfei QiMei DengYouliang ZhengYouliang ZhengHouyang KangHouyang KangYunfeng JiangGuoyue ChenGuoyue ChenFrontiers Media S.A.articleadult-plant resistanceQTLstripe rustChinese wheat landracegenetic mappingheterogeneous inbred familyPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adult-plant resistance QTL stripe rust Chinese wheat landrace genetic mapping heterogeneous inbred family Plant culture SB1-1110
spellingShingle	adult-plant resistance QTL stripe rust Chinese wheat landrace genetic mapping heterogeneous inbred family Plant culture SB1-1110 Yuqi Wang Yuqi Wang Fengying Liang Fangnian Guan Fangjie Yao Li Long Xuyang Zhao Luyao Duan Yu Wu Hao Li Wei Li Qiantao Jiang Qiantao Jiang Yuming Wei Yuming Wei Jian Ma Pengfei Qi Mei Deng Youliang Zheng Youliang Zheng Houyang Kang Houyang Kang Yunfeng Jiang Guoyue Chen Guoyue Chen Molecular Mapping and Analysis of an Excellent Quantitative Trait Loci Conferring Adult-Plant Resistance to Stripe Rust in Chinese Wheat Landrace Gaoxianguangtoumai
description	The Chinese wheat landrace “Gaoxianguangtoumai” (GX) has exhibited a high level of adult-plant resistance (APR) to stripe rust in the field for more than a decade. To reveal the genetic background for APR to stripe rust in GX, a set of 249 F6:8 (F6, F7, and F8) recombinant inbred lines (RILs) was developed from a cross between GX and the susceptible cultivar “Taichung 29.” The parents and RILs were evaluated for disease severity at the adult-plant stage in the field by artificial inoculation with the currently predominant Chinese Puccinia striiformis f. sp. tritici races during three cropping seasons and genotyped using the Wheat 55K single-nucleotide polymorphism (SNP) array to construct a genetic map with 1,871 SNP markers finally. Two stable APR quantitative trait loci (QTL), QYr.GX-2AS and QYr.GX-7DS in GX, were detected on chromosomes 2AS and 7DS, which explained 15.5–27.0% and 11.5–13.5% of the total phenotypic variation, respectively. Compared with published Yr genes and QTL, QYr.GX-7DS and Yr18 may be the same, whereas QYr.GX-2AS is likely to be novel. Haplotype analysis revealed that QYr.GX-2AS is likely to be rare which presents in 5.3% of the 325 surveyed Chinese wheat landraces. By analyzing a heterogeneous inbred family (HIF) population from a residual heterozygous plant in an F8 generation of RIL, QYr.GX-2AS was further flanked by KP2A_36.85 and KP2A_38.22 with a physical distance of about 1.37Mb and co-segregated with the KP2A_37.09. Furthermore, three tightly linked Kompetitive allele-specific PCR (KASP) markers were highly polymorphic among 109 Chinese wheat cultivars. The results of this study can be used in wheat breeding for improving resistance to stripe rust.
format	article
author	Yuqi Wang Yuqi Wang Fengying Liang Fangnian Guan Fangjie Yao Li Long Xuyang Zhao Luyao Duan Yu Wu Hao Li Wei Li Qiantao Jiang Qiantao Jiang Yuming Wei Yuming Wei Jian Ma Pengfei Qi Mei Deng Youliang Zheng Youliang Zheng Houyang Kang Houyang Kang Yunfeng Jiang Guoyue Chen Guoyue Chen
author_facet	Yuqi Wang Yuqi Wang Fengying Liang Fangnian Guan Fangjie Yao Li Long Xuyang Zhao Luyao Duan Yu Wu Hao Li Wei Li Qiantao Jiang Qiantao Jiang Yuming Wei Yuming Wei Jian Ma Pengfei Qi Mei Deng Youliang Zheng Youliang Zheng Houyang Kang Houyang Kang Yunfeng Jiang Guoyue Chen Guoyue Chen
author_sort	Yuqi Wang
title	Molecular Mapping and Analysis of an Excellent Quantitative Trait Loci Conferring Adult-Plant Resistance to Stripe Rust in Chinese Wheat Landrace Gaoxianguangtoumai
title_short	Molecular Mapping and Analysis of an Excellent Quantitative Trait Loci Conferring Adult-Plant Resistance to Stripe Rust in Chinese Wheat Landrace Gaoxianguangtoumai
title_full	Molecular Mapping and Analysis of an Excellent Quantitative Trait Loci Conferring Adult-Plant Resistance to Stripe Rust in Chinese Wheat Landrace Gaoxianguangtoumai
title_fullStr	Molecular Mapping and Analysis of an Excellent Quantitative Trait Loci Conferring Adult-Plant Resistance to Stripe Rust in Chinese Wheat Landrace Gaoxianguangtoumai
title_full_unstemmed	Molecular Mapping and Analysis of an Excellent Quantitative Trait Loci Conferring Adult-Plant Resistance to Stripe Rust in Chinese Wheat Landrace Gaoxianguangtoumai
title_sort	molecular mapping and analysis of an excellent quantitative trait loci conferring adult-plant resistance to stripe rust in chinese wheat landrace gaoxianguangtoumai
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/73da255517314ac8a0b4f2056f181005
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Molecular Mapping and Analysis of an Excellent Quantitative Trait Loci Conferring Adult-Plant Resistance to Stripe Rust in Chinese Wheat Landrace Gaoxianguangtoumai

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