Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity

Minimizing off-target effects is an important concern for therapeutic applications of CRISPR-Cas9. Here, the authors show that incorporating bridged or locked nucleic acids into crRNA improves editing kinetics and reduces off-target cleavage.

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Autores principales:	Christopher R. Cromwell, Keewon Sung, Jinho Park, Amanda R. Krysler, Juan Jovel, Seong Keun Kim, Basil P. Hubbard
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/73e57431c7cf40ba9361ba99c2c9360d
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spelling	oai:doaj.org-article:73e57431c7cf40ba9361ba99c2c9360d2021-12-02T16:56:56ZIncorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity10.1038/s41467-018-03927-02041-1723https://doaj.org/article/73e57431c7cf40ba9361ba99c2c9360d2018-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03927-0https://doaj.org/toc/2041-1723Minimizing off-target effects is an important concern for therapeutic applications of CRISPR-Cas9. Here, the authors show that incorporating bridged or locked nucleic acids into crRNA improves editing kinetics and reduces off-target cleavage.Christopher R. CromwellKeewon SungJinho ParkAmanda R. KryslerJuan JovelSeong Keun KimBasil P. HubbardNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Christopher R. Cromwell Keewon Sung Jinho Park Amanda R. Krysler Juan Jovel Seong Keun Kim Basil P. Hubbard Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
description	Minimizing off-target effects is an important concern for therapeutic applications of CRISPR-Cas9. Here, the authors show that incorporating bridged or locked nucleic acids into crRNA improves editing kinetics and reduces off-target cleavage.
format	article
author	Christopher R. Cromwell Keewon Sung Jinho Park Amanda R. Krysler Juan Jovel Seong Keun Kim Basil P. Hubbard
author_facet	Christopher R. Cromwell Keewon Sung Jinho Park Amanda R. Krysler Juan Jovel Seong Keun Kim Basil P. Hubbard
author_sort	Christopher R. Cromwell
title	Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
title_short	Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
title_full	Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
title_fullStr	Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
title_full_unstemmed	Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
title_sort	incorporation of bridged nucleic acids into crispr rnas improves cas9 endonuclease specificity
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/73e57431c7cf40ba9361ba99c2c9360d
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Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity

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