Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity

Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity

Minimizing off-target effects is an important concern for therapeutic applications of CRISPR-Cas9. Here, the authors show that incorporating bridged or locked nucleic acids into crRNA improves editing kinetics and reduces off-target cleavage.

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Autores principales: Christopher R. Cromwell, Keewon Sung, Jinho Park, Amanda R. Krysler, Juan Jovel, Seong Keun Kim, Basil P. Hubbard
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
Materias:
Science
Q
Acceso en línea:https://doaj.org/article/73e57431c7cf40ba9361ba99c2c9360d
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