The Potentiality of Human Umbilical Cord Isolated Mesenchymal Stem/Stromal Cells for Cardiomyocyte Generation

Amoura Abou-ElNaga,1 Farha El-Chennawi,2 Samar Ibrahim kamel,1 Ghada Mutawa3 1Zoology Department, Faculty of Sciences, Mansoura University, Mansoura 35516, Egypt; 2Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt; 3Department of Basic Science, Faculty of...

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Autores principales:	Abou-ElNaga A, El-Chennawi F, Ibrahim kamel S, Mutawa G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	mesenchymal stem/stromal cells human umbilical cord wharton’s jelly cardiomyocytes 5-azacytidine fibroblast growth factor. Cytology QH573-671
Acceso en línea:	https://doaj.org/article/73e78ae1aa6848deb3a220ad27fbfae9
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spelling	oai:doaj.org-article:73e78ae1aa6848deb3a220ad27fbfae92021-12-02T12:56:54ZThe Potentiality of Human Umbilical Cord Isolated Mesenchymal Stem/Stromal Cells for Cardiomyocyte Generation1178-6957https://doaj.org/article/73e78ae1aa6848deb3a220ad27fbfae92020-11-01T00:00:00Zhttps://www.dovepress.com/the-potentiality-of-human-umbilical-cord-isolated-mesenchymal-stemstro-peer-reviewed-article-SCCAAhttps://doaj.org/toc/1178-6957Amoura Abou-ElNaga,1 Farha El-Chennawi,2 Samar Ibrahim kamel,1 Ghada Mutawa3 1Zoology Department, Faculty of Sciences, Mansoura University, Mansoura 35516, Egypt; 2Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt; 3Department of Basic Science, Faculty of Dentistry, Horus University in Egypt (HUE), New Damietta 34518, EgyptCorrespondence: Ghada MutawaDepartment of Basic Science, Faculty of Dentistry, Horus University in Egypt (HUE), International Coastal Road New, Damietta City, Kafr Saad, Damietta Governorate 34518, EgyptTel +201021888628Fax +2-057-328611Email Ghada.adel.mutawa@gmail.comBackground: The new therapeutic strategy of managing cardiac diseases is based on cell therapy; it highly suggests the use of multipotent mesenchymal stem/stromal cells (MSCs). MSCs widely used in researches are known to be isolated from bone marrow. However, this research seeks to use a human umbilical cord (HUC) as an alternative source of MSCs. Since HUC Wharton’s jelly (WJ)-isolated MSCs originate as fetal tissue they are highly preferable for their potential advantages over other adult tissues.Methods: The researchers used enzymatic digestion to establish a primary HUC-WJ-isolated MSC line. Then, flow cytometry was used to characterize MSCs and hematopoietic stem cells (HSCs) markers’ expression. In addition, the cardiac differentiation capacity of HUC-WJ-isolated MSCs in vitro was investigated by two protocols. Protocol-1 necessitates the dependence on merely 5-azacytidine (5-Aza), whereas in protocol-2, 5-Aza was supported by basic fibroblast growth factor (BFGF). The comparative study between the two protocols was applied by inspecting the ultrastructure of differentiated cells, measuring RT-PCR mRNA cardiac markers and the quantitative detection of cardiac proteins.Results: HUC-WJ isolated MSCs were expressed by CD90+ve, CD105+ve, CD106+ve, CD45−ve, and CD146−ve. Remarkable TNNT1, NKX2.5, and Desmin mRNA expression and higher quantitative LDH and cTnI were detected by applying protocol-2. This same protocol-2 induced cardiac morphological features that were revealed by identifying cardiomyocyte-like cells and typical sarcomeres.Conclusion: HUC-WJ is proved to be an ethical and effective source of MSCs induced cardiac differentiation, whereas BFGF supports 5-Aza in MSCs-cardiomyocytes differentiation.Keywords: mesenchymal stem/stromal cells, human umbilical cord, Wharton’s jelly, cardiomyocytes, 5-azacytidine, fibroblast growth factorAbou-ElNaga AEl-Chennawi FIbrahim kamel SMutawa GDove Medical Pressarticlemesenchymal stem/stromal cellshuman umbilical cordwharton’s jellycardiomyocytes5-azacytidinefibroblast growth factor.CytologyQH573-671ENStem Cells and Cloning: Advances and Applications, Vol Volume 13, Pp 91-101 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	mesenchymal stem/stromal cells human umbilical cord wharton’s jelly cardiomyocytes 5-azacytidine fibroblast growth factor. Cytology QH573-671
spellingShingle	mesenchymal stem/stromal cells human umbilical cord wharton’s jelly cardiomyocytes 5-azacytidine fibroblast growth factor. Cytology QH573-671 Abou-ElNaga A El-Chennawi F Ibrahim kamel S Mutawa G The Potentiality of Human Umbilical Cord Isolated Mesenchymal Stem/Stromal Cells for Cardiomyocyte Generation
description	Amoura Abou-ElNaga,1 Farha El-Chennawi,2 Samar Ibrahim kamel,1 Ghada Mutawa3 1Zoology Department, Faculty of Sciences, Mansoura University, Mansoura 35516, Egypt; 2Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt; 3Department of Basic Science, Faculty of Dentistry, Horus University in Egypt (HUE), New Damietta 34518, EgyptCorrespondence: Ghada MutawaDepartment of Basic Science, Faculty of Dentistry, Horus University in Egypt (HUE), International Coastal Road New, Damietta City, Kafr Saad, Damietta Governorate 34518, EgyptTel +201021888628Fax +2-057-328611Email Ghada.adel.mutawa@gmail.comBackground: The new therapeutic strategy of managing cardiac diseases is based on cell therapy; it highly suggests the use of multipotent mesenchymal stem/stromal cells (MSCs). MSCs widely used in researches are known to be isolated from bone marrow. However, this research seeks to use a human umbilical cord (HUC) as an alternative source of MSCs. Since HUC Wharton’s jelly (WJ)-isolated MSCs originate as fetal tissue they are highly preferable for their potential advantages over other adult tissues.Methods: The researchers used enzymatic digestion to establish a primary HUC-WJ-isolated MSC line. Then, flow cytometry was used to characterize MSCs and hematopoietic stem cells (HSCs) markers’ expression. In addition, the cardiac differentiation capacity of HUC-WJ-isolated MSCs in vitro was investigated by two protocols. Protocol-1 necessitates the dependence on merely 5-azacytidine (5-Aza), whereas in protocol-2, 5-Aza was supported by basic fibroblast growth factor (BFGF). The comparative study between the two protocols was applied by inspecting the ultrastructure of differentiated cells, measuring RT-PCR mRNA cardiac markers and the quantitative detection of cardiac proteins.Results: HUC-WJ isolated MSCs were expressed by CD90+ve, CD105+ve, CD106+ve, CD45−ve, and CD146−ve. Remarkable TNNT1, NKX2.5, and Desmin mRNA expression and higher quantitative LDH and cTnI were detected by applying protocol-2. This same protocol-2 induced cardiac morphological features that were revealed by identifying cardiomyocyte-like cells and typical sarcomeres.Conclusion: HUC-WJ is proved to be an ethical and effective source of MSCs induced cardiac differentiation, whereas BFGF supports 5-Aza in MSCs-cardiomyocytes differentiation.Keywords: mesenchymal stem/stromal cells, human umbilical cord, Wharton’s jelly, cardiomyocytes, 5-azacytidine, fibroblast growth factor
format	article
author	Abou-ElNaga A El-Chennawi F Ibrahim kamel S Mutawa G
author_facet	Abou-ElNaga A El-Chennawi F Ibrahim kamel S Mutawa G
author_sort	Abou-ElNaga A
title	The Potentiality of Human Umbilical Cord Isolated Mesenchymal Stem/Stromal Cells for Cardiomyocyte Generation
title_short	The Potentiality of Human Umbilical Cord Isolated Mesenchymal Stem/Stromal Cells for Cardiomyocyte Generation
title_full	The Potentiality of Human Umbilical Cord Isolated Mesenchymal Stem/Stromal Cells for Cardiomyocyte Generation
title_fullStr	The Potentiality of Human Umbilical Cord Isolated Mesenchymal Stem/Stromal Cells for Cardiomyocyte Generation
title_full_unstemmed	The Potentiality of Human Umbilical Cord Isolated Mesenchymal Stem/Stromal Cells for Cardiomyocyte Generation
title_sort	potentiality of human umbilical cord isolated mesenchymal stem/stromal cells for cardiomyocyte generation
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/73e78ae1aa6848deb3a220ad27fbfae9
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The Potentiality of Human Umbilical Cord Isolated Mesenchymal Stem/Stromal Cells for Cardiomyocyte Generation

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