Potential Antioxidant and Anticancer Activities of Secondary Metabolites of <i>Nostoc linckia</i> Cultivated under Zn and Cu Stress Conditions

The objective of the present study is to determine the antioxidant and anticancer activities of <i>Nostoc linckia</i> extracts cultivated under heavy metal stress conditions (0.44, 0.88, and 1.76 mg/L for zinc and 0.158, 0.316, 0.632 mg/L for copper). Phycobiliprotein, phenolic compounds...

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Autores principales:	Khaled M. A. Ramadan, Hossam S. El-Beltagi, Sanaa M. M. Shanab, Eman A. El-fayoumy, Emad A. Shalaby, Eslam S. A. Bendary
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Nostoc linckia</i> chemical compositions oxidative stress anticancer activity antioxidant activity Chemical technology TP1-1185 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/73e7fd5e6c3340758cc5762156cab303
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spelling	oai:doaj.org-article:73e7fd5e6c3340758cc5762156cab3032021-11-25T18:51:02ZPotential Antioxidant and Anticancer Activities of Secondary Metabolites of <i>Nostoc linckia</i> Cultivated under Zn and Cu Stress Conditions10.3390/pr91119722227-9717https://doaj.org/article/73e7fd5e6c3340758cc5762156cab3032021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9717/9/11/1972https://doaj.org/toc/2227-9717The objective of the present study is to determine the antioxidant and anticancer activities of <i>Nostoc linckia</i> extracts cultivated under heavy metal stress conditions (0.44, 0.88, and 1.76 mg/L for zinc and 0.158, 0.316, 0.632 mg/L for copper). Phycobiliprotein, phenolic compounds, flavonoids, and tannins were measured. Active ingredients of extracts were evaluated by GC-mass spectroscopy. The obtained results revealed that higher zinc and copper concentrations showed growth inhibition while 0.22 mg/L (Zn) and 0.079 mg/L (Cu) enhanced growth, reaching its maximum on the 25th day. Increases in catalase, lipids peroxidation, and antioxidants, as well as tannins and flavonoids, have been induced by integration of 0.88 mg/L (Zn) and 0.316 mg/L (Cu). Elevation of Zn concentration induced augmentation of antioxidant activity of crude extract (DPPH or ABTS), with superior activity at 0.44 mg/L zinc concentration (81.22%). The anticancer activity of <i>Nostoc</i> <i>linckia</i> extract (0.44 mg/L Zn) tested against four cancer cell lines: A549, Hela, HCT 116, and MCF-7. The extract at 500 µg/mL appeared the lowest cell viability of tested cell lines. The promising extract (0.44 mg/L Zn) recorded the lowest cell viability of 25.57% in cervical cell line, 29.74% in breast cell line, 33.10% in lung cell line and 34.53% in the colon cell line. The antioxidant active extract showed significant stability against pH with attributed increase in antioxidant activity in the range between 8–12. The extract can be used effectively as a natural antioxidant and anticancer after progressive testing.Khaled M. A. RamadanHossam S. El-BeltagiSanaa M. M. ShanabEman A. El-fayoumyEmad A. ShalabyEslam S. A. BendaryMDPI AGarticle<i>Nostoc linckia</i>chemical compositionsoxidative stressanticancer activityantioxidant activityChemical technologyTP1-1185ChemistryQD1-999ENProcesses, Vol 9, Iss 1972, p 1972 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Nostoc linckia</i> chemical compositions oxidative stress anticancer activity antioxidant activity Chemical technology TP1-1185 Chemistry QD1-999
spellingShingle	<i>Nostoc linckia</i> chemical compositions oxidative stress anticancer activity antioxidant activity Chemical technology TP1-1185 Chemistry QD1-999 Khaled M. A. Ramadan Hossam S. El-Beltagi Sanaa M. M. Shanab Eman A. El-fayoumy Emad A. Shalaby Eslam S. A. Bendary Potential Antioxidant and Anticancer Activities of Secondary Metabolites of <i>Nostoc linckia</i> Cultivated under Zn and Cu Stress Conditions
description	The objective of the present study is to determine the antioxidant and anticancer activities of <i>Nostoc linckia</i> extracts cultivated under heavy metal stress conditions (0.44, 0.88, and 1.76 mg/L for zinc and 0.158, 0.316, 0.632 mg/L for copper). Phycobiliprotein, phenolic compounds, flavonoids, and tannins were measured. Active ingredients of extracts were evaluated by GC-mass spectroscopy. The obtained results revealed that higher zinc and copper concentrations showed growth inhibition while 0.22 mg/L (Zn) and 0.079 mg/L (Cu) enhanced growth, reaching its maximum on the 25th day. Increases in catalase, lipids peroxidation, and antioxidants, as well as tannins and flavonoids, have been induced by integration of 0.88 mg/L (Zn) and 0.316 mg/L (Cu). Elevation of Zn concentration induced augmentation of antioxidant activity of crude extract (DPPH or ABTS), with superior activity at 0.44 mg/L zinc concentration (81.22%). The anticancer activity of <i>Nostoc</i> <i>linckia</i> extract (0.44 mg/L Zn) tested against four cancer cell lines: A549, Hela, HCT 116, and MCF-7. The extract at 500 µg/mL appeared the lowest cell viability of tested cell lines. The promising extract (0.44 mg/L Zn) recorded the lowest cell viability of 25.57% in cervical cell line, 29.74% in breast cell line, 33.10% in lung cell line and 34.53% in the colon cell line. The antioxidant active extract showed significant stability against pH with attributed increase in antioxidant activity in the range between 8–12. The extract can be used effectively as a natural antioxidant and anticancer after progressive testing.
format	article
author	Khaled M. A. Ramadan Hossam S. El-Beltagi Sanaa M. M. Shanab Eman A. El-fayoumy Emad A. Shalaby Eslam S. A. Bendary
author_facet	Khaled M. A. Ramadan Hossam S. El-Beltagi Sanaa M. M. Shanab Eman A. El-fayoumy Emad A. Shalaby Eslam S. A. Bendary
author_sort	Khaled M. A. Ramadan
title	Potential Antioxidant and Anticancer Activities of Secondary Metabolites of <i>Nostoc linckia</i> Cultivated under Zn and Cu Stress Conditions
title_short	Potential Antioxidant and Anticancer Activities of Secondary Metabolites of <i>Nostoc linckia</i> Cultivated under Zn and Cu Stress Conditions
title_full	Potential Antioxidant and Anticancer Activities of Secondary Metabolites of <i>Nostoc linckia</i> Cultivated under Zn and Cu Stress Conditions
title_fullStr	Potential Antioxidant and Anticancer Activities of Secondary Metabolites of <i>Nostoc linckia</i> Cultivated under Zn and Cu Stress Conditions
title_full_unstemmed	Potential Antioxidant and Anticancer Activities of Secondary Metabolites of <i>Nostoc linckia</i> Cultivated under Zn and Cu Stress Conditions
title_sort	potential antioxidant and anticancer activities of secondary metabolites of <i>nostoc linckia</i> cultivated under zn and cu stress conditions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/73e7fd5e6c3340758cc5762156cab303
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Potential Antioxidant and Anticancer Activities of Secondary Metabolites of <i>Nostoc linckia</i> Cultivated under Zn and Cu Stress Conditions

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