Matrix rigidity-modulated cardiovascular organoid formation from embryoid bodies.

Stem cell clusters, such as embryoid bodies (EBs) derived from embryonic stem cells, are extensively studied for creation of multicellular clusters and complex functional tissues. It is common to control phenotypes of ES cells with varying molecular compounds; however, there is still a need to impro...

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Autores principales:	Artem Shkumatov, Kwanghyun Baek, Hyunjoon Kong
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/73ea6a05e2c94a49b5ebb7a3dbf27c90
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spelling	oai:doaj.org-article:73ea6a05e2c94a49b5ebb7a3dbf27c902021-11-18T08:23:29ZMatrix rigidity-modulated cardiovascular organoid formation from embryoid bodies.1932-620310.1371/journal.pone.0094764https://doaj.org/article/73ea6a05e2c94a49b5ebb7a3dbf27c902014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24732893/?tool=EBIhttps://doaj.org/toc/1932-6203Stem cell clusters, such as embryoid bodies (EBs) derived from embryonic stem cells, are extensively studied for creation of multicellular clusters and complex functional tissues. It is common to control phenotypes of ES cells with varying molecular compounds; however, there is still a need to improve the controllability of cell differentiation, and thus, the quality of created tissue. This study demonstrates a simple but effective strategy to promote formation of vascularized cardiac muscle-like tissue in EBs and form contracting cardiovascular organoids by modulating the stiffness of a cell adherent hydrogel. Using collagen-conjugated polyacrylamide hydrogels with controlled elastic moduli, we discovered that cellular organization in a form of vascularized cardiac muscle sheet was maximal on the gel with the stiffness similar to cardiac muscle. We envisage that the results of this study will greatly contribute to better understanding of emergent behavior of stem cells in developmental and regeneration process and will also expedite translation of EB studies to drug-screening device assembly and clinical treatments.Artem ShkumatovKwanghyun BaekHyunjoon KongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e94764 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Artem Shkumatov Kwanghyun Baek Hyunjoon Kong Matrix rigidity-modulated cardiovascular organoid formation from embryoid bodies.
description	Stem cell clusters, such as embryoid bodies (EBs) derived from embryonic stem cells, are extensively studied for creation of multicellular clusters and complex functional tissues. It is common to control phenotypes of ES cells with varying molecular compounds; however, there is still a need to improve the controllability of cell differentiation, and thus, the quality of created tissue. This study demonstrates a simple but effective strategy to promote formation of vascularized cardiac muscle-like tissue in EBs and form contracting cardiovascular organoids by modulating the stiffness of a cell adherent hydrogel. Using collagen-conjugated polyacrylamide hydrogels with controlled elastic moduli, we discovered that cellular organization in a form of vascularized cardiac muscle sheet was maximal on the gel with the stiffness similar to cardiac muscle. We envisage that the results of this study will greatly contribute to better understanding of emergent behavior of stem cells in developmental and regeneration process and will also expedite translation of EB studies to drug-screening device assembly and clinical treatments.
format	article
author	Artem Shkumatov Kwanghyun Baek Hyunjoon Kong
author_facet	Artem Shkumatov Kwanghyun Baek Hyunjoon Kong
author_sort	Artem Shkumatov
title	Matrix rigidity-modulated cardiovascular organoid formation from embryoid bodies.
title_short	Matrix rigidity-modulated cardiovascular organoid formation from embryoid bodies.
title_full	Matrix rigidity-modulated cardiovascular organoid formation from embryoid bodies.
title_fullStr	Matrix rigidity-modulated cardiovascular organoid formation from embryoid bodies.
title_full_unstemmed	Matrix rigidity-modulated cardiovascular organoid formation from embryoid bodies.
title_sort	matrix rigidity-modulated cardiovascular organoid formation from embryoid bodies.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/73ea6a05e2c94a49b5ebb7a3dbf27c90
work_keys_str_mv	AT artemshkumatov matrixrigiditymodulatedcardiovascularorganoidformationfromembryoidbodies AT kwanghyunbaek matrixrigiditymodulatedcardiovascularorganoidformationfromembryoidbodies AT hyunjoonkong matrixrigiditymodulatedcardiovascularorganoidformationfromembryoidbodies
_version_	1718421866753818624

Matrix rigidity-modulated cardiovascular organoid formation from embryoid bodies.

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